![]() |
|
Annotation rule MF_04150 |
Accession | MF_04150 |
Dates | 6-MAR-2020 (Created) 7-APR-2020 (Last updated, Version 2) |
Name | RNALIG2_T4 |
Scope | Viruses |
Template | P32277 (RLIG2_BPT4) |
Triggered by |
Protein name |
|
Protein name |
|
Function | Repairs 3'-OH/5'-PO4 nicks in duplex RNA or RNA:DNA hybrid in which the broken 3'-OH strand is RNA. The nick ligation reaction entails three nucleotidyl transfer steps. In the first step, the RNA ligase reacts with ATP in the absence of nucleic acid to form a covalent ligase-AMP intermediate and release pyrophosphate. In step 2, the ligase-AMP binds to the nicked duplex nucleic acid and transfers the adenylate to the 5'-PO4 terminus to form an adenylylated nicked intermediate. In step 3, the RNA ligase directs the attack of the nick 3'-OH on the 5'-phosphoanhydride linkage, resulting in a repaired 3' - 5' phosphodiester and release of AMP. |
Catalytic activity | Reaction=ATP + (ribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(ribonucleotide)(m) = (ribonucleotide)(n+m) + AMP + diphosphate.; EC=6.5.1.3; |
Cofactor | Mg(2+) Mn(2+) Note: Binds 2 magnesium ions that may perform the catalytic activity via a two-metal mechanism; |
Domain | The adenylyltransferase domain in the N-terminus performs step 1 and step 3 reactions. The C-terminus domain is required for step 2 of the ligation pathway. |
Similarity | Belongs to the RNA ligase 2 family. |
From: RLIG2_BPT4 (P32277) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
REGION | 1 | 234 | Adenylyltransferase | |||||||||
ACT_SITE | 35 | 35 | N6-AMP-lysine intermediate | K | 1 | |||||||
METAL | 204 | 204 | Magnesium | E | 1 |
BINDING | 34 | 34 | AMP | E | ||||||||
BINDING | 36 | 36 | AMP | [IL] | ||||||||
BINDING | 40 | 40 | AMP | N | ||||||||
BINDING | 55 | 55 | AMP | R | ||||||||
BINDING | 99 | 99 | AMP | E | ||||||||
BINDING | 225 | 225 | AMP | K | ||||||||
BINDING | 227 | 227 | AMP | K |
Size range | 270-360 amino acids |
Related rules | None |
Fusion | None |