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HAMAP rule MF_04152

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General rule information [?]

Accession MF_04152
Dates 12-JAN-2021 (Created)
19-NOV-2022 (Last updated, Version 5)
Name SSB_T4
Scope(s) Viruses
Template(s) P03695 (SSB_BPT4); [ Recover all ]
Triggered by HAMAP; MF_04152 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier SSB
case <OC:Tequatrovirus>
Protein name RecName: Full=Single-stranded DNA-binding protein;
                 Short=SSB protein;
AltName: Full=Gp32;
AltName: Full=Helix-destabilizing protein;
Gene name Name=32; Synonyms=ssb;
else
Protein name RecName: Full=Single-stranded DNA-binding protein;
                 Short=SSB protein;
AltName: Full=Helix-destabilizing protein;
end case

Comments [?]

case <OC:Tequatrovirus>
FUNCTIONSingle-stranded DNA-binding protein that participates in viral DNA replication, recombination, and repair. Coats the lagging- strand ssDNA as the replication fork advances. Stimulates the activities of viral DNA polymerase and DnaB-like SF4 replicative helicase, probably via its interaction with the helicase assembly factor. Together with DnaB-like SF4 replicative helicase and the helicase assembly factor, promotes pairing of two homologous DNA molecules containing complementary single-stranded regions and mediates homologous DNA strand exchange. Promotes also the formation of joint molecules. mRNA specific autogenous translational repressor.
SUBUNITHomodimer in the absence of DNA, monomer when binding DNA. Interacts with the DNA helicase assembly protein; a ternary complex between the helicase assembly protein, the single-stranded DNA-binding protein and ssDNA is an obligatory intermediate in the helicase loading mechanism. Part of the replicase complex that includes the DNA polymerase, the polymerase clamp, the clamp loader complex, the single- stranded DNA binding protein, the primase, the DnaB-like SF4 replicative helicase and the helicase assembly factor. Interacts (via C-terminus) with the viral SF1 dDA helicase. Interacts with the viral SF2 UvsW repair helicase.
SIMILARITYBelongs to the Tequatrovirus single-stranded DNA-binding protein family.
else
FUNCTIONSingle-stranded DNA-binding protein that participates in viral DNA replication, recombination, and repair. Coats the lagging- strand ssDNA as the replication fork advances. Stimulates the activities of viral DNA polymerase and the replicative helicase, probably via its interaction with the helicase assembly factor. Together with the replicative helicase and the helicase assembly factor, promotes pairing of two homologous DNA molecules containing complementary single-stranded regions and mediates homologous DNA strand exchange. Promotes also the formation of joint molecules. mRNA specific autogenous translational repressor.
SUBUNITHomodimer in the absence of DNA, monomer when binding DNA. Interacts with the DNA helicase assembly protein; a ternary complex between the helicase assembly protein, the single-stranded DNA-binding protein and ssDNA is an obligatory intermediate in the helicase loading mechanism. Part of the replicase complex that includes the DNA polymerase, the polymerase clamp, the clamp loader complex, the single- stranded DNA binding protein, the primase, the replicative helicase and the helicase assembly factor. Interacts (via C-terminus) with the viral SF1 dDA helicase. Interacts with the viral SF2 UvsW repair helicase.
DOMAINThe acidic C-terminus is involved in modulating the ssDNA binding properties. The N-terminus LAST motif is involved in the cooperative binding of the protein to single-stranded nucleic acids.
SIMILARITYBelongs to the Tequatrovirus single-stranded DNA-binding protein family.
end case
case <FTTag:LAST>
DOMAINThe acidic C-terminus is involved in modulating the ssDNA binding properties. The N-terminus LAST motif is involved in the cooperative binding of the protein to ssDNA.
else
DOMAINThe acidic C-terminus is involved in modulating the ssDNA binding properties.
end case

Keywords [?]

DNA recombination
DNA repair
DNA-binding
Repressor
Viral DNA replication
case <FTGroup:1>
Metal-binding
Zinc
end case

Gene Ontology [?]

case <FTGroup:1>
GO:0046872; Molecular function:metal ion binding
end case
GO:0003697; Molecular function:single-stranded DNA binding
GO:0039686; Biological process:bidirectional double-stranded viral DNA replication
GO:0006310; Biological process:DNA recombination
GO:0006281; Biological process:DNA repair
GO:0039693; Biological process:viral DNA genome replication

Cross-references [?]

Pfam PF08804; gp32; 1;

Features [?]

From: SSB_BPT4 (P03695)
Key From To Description Tag Condition FTGroup
REGION 3 7 /note="LAST" LAST [KR]-[KR]-[KR]-x-x
BINDING 64 64 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"		 H 1
BINDING 77 77 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"		 C 1
BINDING 87 87 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"		 C 1
BINDING 90 90 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"		 C 1
From: PRU00004
Key From To Description Tag Condition FTGroup

Additional information [?]

Size range 200-450 amino acids
Related rules None
Fusion Nter: None Cter: None



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