HAMAP rule MF_04152
General rule information
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Accession | MF_04152 |
Dates | 12-JAN-2021 (Created) 19-NOV-2022 (Last updated, Version 5) |
Name | SSB_T4 |
Scope | Viruses |
Template | P03695 (SSB_BPT4) |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
|
case <OC:Tequatrovirus>
Protein name |
|
Gene name |
|
else
Protein name |
|
end case
Comments
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case <OC:Tequatrovirus>
Function | Single-stranded DNA-binding protein that participates in viral DNA replication, recombination, and repair. Coats the lagging-strand ssDNA as the replication fork advances. Stimulates the activities of viral DNA polymerase and DnaB-like SF4 replicative helicase, probably via its interaction with the helicase assembly factor. Together with DnaB-like SF4 replicative helicase and the helicase assembly factor, promotes pairing of two homologous DNA molecules containing complementary single-stranded regions and mediates homologous DNA strand exchange. Promotes also the formation of joint molecules. mRNA specific autogenous translational repressor. |
Subunit | Homodimer in the absence of DNA, monomer when binding DNA. Interacts with the DNA helicase assembly protein; a ternary complex between the helicase assembly protein, the single-stranded DNA-binding protein and ssDNA is an obligatory intermediate in the helicase loading mechanism. Part of the replicase complex that includes the DNA polymerase, the polymerase clamp, the clamp loader complex, the single-stranded DNA binding protein, the primase, the DnaB-like SF4 replicative helicase and the helicase assembly factor. Interacts (via C-terminus) with the viral SF1 dDA helicase. Interacts with the viral SF2 UvsW repair helicase. |
Similarity | Belongs to the Tequatrovirus single-stranded DNA-binding protein family. |
else
Function | Single-stranded DNA-binding protein that participates in viral DNA replication, recombination, and repair. Coats the lagging-strand ssDNA as the replication fork advances. Stimulates the activities of viral DNA polymerase and the replicative helicase, probably via its interaction with the helicase assembly factor. Together with the replicative helicase and the helicase assembly factor, promotes pairing of two homologous DNA molecules containing complementary single-stranded regions and mediates homologous DNA strand exchange. Promotes also the formation of joint molecules. mRNA specific autogenous translational repressor. |
Subunit | Homodimer in the absence of DNA, monomer when binding DNA. Interacts with the DNA helicase assembly protein; a ternary complex between the helicase assembly protein, the single-stranded DNA-binding protein and ssDNA is an obligatory intermediate in the helicase loading mechanism. Part of the replicase complex that includes the DNA polymerase, the polymerase clamp, the clamp loader complex, the single-stranded DNA binding protein, the primase, the replicative helicase and the helicase assembly factor. Interacts (via C-terminus) with the viral SF1 dDA helicase. Interacts with the viral SF2 UvsW repair helicase. |
Domain | The acidic C-terminus is involved in modulating the ssDNA binding properties. The N-terminus LAST motif is involved in the cooperative binding of the protein to single-stranded nucleic acids. |
Similarity | Belongs to the Tequatrovirus single-stranded DNA-binding protein family. |
end case
case <FTTag:LAST>
Domain | The acidic C-terminus is involved in modulating the ssDNA binding properties. The N-terminus LAST motif is involved in the cooperative binding of the protein to ssDNA. |
else
Domain | The acidic C-terminus is involved in modulating the ssDNA binding properties. |
end case
Keywords
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case <FTGroup:1>
end case
Gene Ontology
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case <FTGroup:1>
GO:0046872; Molecular function: metal ion binding.
end case
GO:0003697; Molecular function: single-stranded DNA binding.
GO:0039686; Biological process: bidirectional double-stranded viral DNA replication.
GO:0006310; Biological process: DNA recombination.
GO:0006281; Biological process: DNA repair.
GO:0039693; Biological process: viral DNA genome replication.
GO:0039686; Biological process: bidirectional double-stranded viral DNA replication.
GO:0006310; Biological process: DNA recombination.
GO:0006281; Biological process: DNA repair.
GO:0039693; Biological process: viral DNA genome replication.
Cross-references
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Pfam | PF08804; gp32; 1; |
Features
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From: SSB_BPT4 (P03695) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
REGION (Optional) | 3 | 7 | LAST | LAST | [KR]-[KR]-[KR]-x-x | |||||||
BINDING | 64 | 64 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | H | 1 | |||||||
BINDING | 77 | 77 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | C | 1 | |||||||
BINDING | 87 | 87 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | C | 1 | |||||||
BINDING | 90 | 90 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | C | 1 |
Additional information
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Size range | 200-450 amino acids |
Related rules | None |
Fusion | None |