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| Annotation rule MF_04166 |
General rule information
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| Accession | MF_04166 |
| Dates | 3-JUN-2021 (Created) 9-JUN-2021 (Last updated, Version 2) |
| Name | Phage_PURZ |
| Scope | Viruses; Caudovirales |
| Template | G3FFN6 (PURZ_BPVC8) |
| Triggered by |
Propagated annotation
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Identifier, protein and gene names
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| Identifier |
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| Protein name |
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| Gene name |
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Comments
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| Function | Involved in the synthesis of the atypical nucleotide dZTP (2-amino-2'-deoxyadenosine-5'-triphosphate). Involved in the synthesis of N6-succino-2-amino-2'-deoxyadenylate, which undergoes defumarylation and phosphorylation respectively by host PurB and guanylate/nucleoside diphosphate kinases to give dZTP (2-amino-2'-deoxyadenosine-5'-triphosphate). dZTP is integrated into the viral genome instead of adenine by the viral polymerase. This Z-base probably completely replaces adenosine and forms a triple bond to the opposite T-base. The resulting non-standard viral DNA is called Z-genome. The chemically modified DNA is probably harder for the host bacteria to digest with nucleases or restriction enzymes. |
| Catalytic activity | RHEA:67628: ATP + dGMP + L-aspartate = (2S)-2-amino-2'-deoxyadenylo-succinate + ADP + 2 H(+) + phosphate |
case <FTGroup:1>
| Cofactor | Mg(2+) |
end case
| Pathway | Purine metabolism. |
| Similarity | Belongs to the Caudovirales PurZ family. |
Keywords
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Gene Ontology
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GO:0005524; Molecular function: ATP binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0006164; Biological process: purine nucleotide biosynthetic process.
GO:0000287; Molecular function: magnesium ion binding.
GO:0006164; Biological process: purine nucleotide biosynthetic process.
Cross-references
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| Pfam | PF00709; Adenylsucc_synt; 0-2; |
Features
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| From: PURZ_BPVC8 (G3FFN6) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 14 | 14 | S | |||||||||
| METAL | 14 | 14 | Magnesium | S | 1 | |||||||
| METAL | 42 | 42 | Magnesium; via carbonyl oxygen | G | 1 | |||||||
| METAL | 263 | 263 | Magnesium | T | 1 | |||||||
| BINDING | 262 | 262 | Substrate | [AT] | ||||||||
| BINDING | 264 | 264 | Substrate; via carbonyl oxygen | V | ||||||||
| BINDING | 269 | 269 | Substrate | R | ||||||||
Additional information
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| Size range | 320-420 amino acids |
| Related rules | None |
| Fusion | None |