HAMAP rule MF_04166
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_04166 |
| Accession | MF_04166 |
| Dates | 03-JUN-2021 (Created)
02-SEP-2024 (Last updated, Version 7) |
| Name | Phage_PURZ |
| Scope(s) |
Viruses Caudoviricetes |
| Template(s) | G3FFN6; [ Recover all ] |
| Triggered by |
HAMAP; MF_04166 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | PURZ |
| Protein name | RecName: Full=N6-succino-2-amino-2'-deoxyadenylate synthase; EC=6.3.4.25; AltName: Full=2-amino-2'-deoxyadenylo-succinate synthase; AltName: Full=PurZ; |
| Gene name | Name=purZ; |
Comments
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| FUNCTION | Involved in the synthesis of the atypical nucleotide dZTP (2- amino-2'-deoxyadenosine-5'-triphosphate). Catalyzes the condensation of aspartate with deoxyguanylate into dSMP (N6-succino-2-amino-2'- deoxyadenylate), which undergoes defumarylation and phosphorylation respectively by host PurB and guanylate/nucleoside diphosphate kinases to give dZTP. dZTP is integrated into the viral genome instead of adenine by the viral DNA polymerase. This Z-base probably completely replaces adenosine and forms a triple bond to the opposite T-base. The resulting non-standard viral DNA is called Z-genome. The chemically modified DNA is probably harder for the host bacteria to digest with nucleases or restriction enzymes. |
| CATALYTIC ACTIVITY | Reaction=dGMP + L-aspartate + ATP = (2S)-2-amino-2'-deoxyadenylo- succinate + ADP + phosphate + 2 H(+); Xref=Rhea:RHEA:67628, ChEBI:CHEBI:15378, ChEBI:CHEBI:29991, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:57673, ChEBI:CHEBI:172924, ChEBI:CHEBI:456216; EC=6.3.4.25; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:67629; |
| case <FTGroup:1> | |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| end case | |
| PATHWAY | Purine metabolism. |
| SIMILARITY | Belongs to the Caudovirales PurZ family. |
Keywords
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Gene Ontology
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| GO:0005524; Molecular function:ATP binding |
| GO:0000287; Molecular function:magnesium ion binding |
| GO:0006164; Biological process:purine nucleotide biosynthetic process |
Cross-references
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| Pfam | PF00709; Adenylsucc_synt; 0-2; |
Features
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| From: PURZ_BPVC8 (G3FFN6) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 14 | 14 | /note="Proton acceptor" | S | ||||||||
| BINDING | 14 | 14 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
S | ||||||||
| BINDING | 14 | 14 | /ligand="dGMP" /ligand_id="ChEBI:CHEBI:57673" |
S | ||||||||
| BINDING | 14 | 14 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
S | 1 | |||||||
| BINDING | 15 | 15 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
T | ||||||||
| BINDING | 16 | 16 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G | ||||||||
| BINDING | 17 | 17 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
K | ||||||||
| BINDING | 18 | 18 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G | ||||||||
| BINDING | 40 | 40 | /ligand="dGMP" /ligand_id="ChEBI:CHEBI:57673" |
N | ||||||||
| BINDING | 42 | 42 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G | ||||||||
| BINDING | 42 | 42 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
G | 1 | |||||||
| BINDING | 43 | 43 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
H | ||||||||
| BINDING | 44 | 44 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
T | ||||||||
| BINDING | 127 | 127 | /ligand="dGMP" /ligand_id="ChEBI:CHEBI:57673" |
S | ||||||||
| BINDING | 128 | 128 | /ligand="dGMP" /ligand_id="ChEBI:CHEBI:57673" |
T | ||||||||
| BINDING | 142 | 142 | /ligand="dGMP" /ligand_id="ChEBI:CHEBI:57673" |
R | ||||||||
| BINDING | 187 | 187 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
Q | ||||||||
| BINDING | 202 | 202 | /ligand="dGMP" /ligand_id="ChEBI:CHEBI:57673" |
T | ||||||||
| BINDING | 263 | 263 | /ligand="L-aspartate" /ligand_id="ChEBI:CHEBI:29991" |
T | ||||||||
| BINDING | 263 | 263 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
T | 1 | |||||||
| BINDING | 264 | 264 | /ligand="L-aspartate" /ligand_id="ChEBI:CHEBI:29991" |
V | ||||||||
| BINDING | 269 | 269 | /ligand="L-aspartate" /ligand_id="ChEBI:CHEBI:29991" |
R | ||||||||
| BINDING | 294 | 294 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
N | ||||||||
| BINDING | 297 | 297 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
N | ||||||||
| BINDING | 330 | 330 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G | ||||||||
Additional information
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| Size range | 320-420 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |