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Annotation rule MF_04166 |
Accession | MF_04166 |
Dates | 3-JUN-2021 (Created) 9-JUN-2021 (Last updated, Version 2) |
Name | Phage_PURZ |
Scope | Viruses; Caudovirales |
Template | G3FFN6 (PURZ_BPVC8) |
Triggered by |
Identifier |
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Protein name |
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Gene name |
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Function | Involved in the synthesis of the atypical nucleotide dZTP (2-amino-2'-deoxyadenosine-5'-triphosphate). Involved in the synthesis of N6-succino-2-amino-2'-deoxyadenylate, which undergoes defumarylation and phosphorylation respectively by host PurB and guanylate/nucleoside diphosphate kinases to give dZTP (2-amino-2'-deoxyadenosine-5'-triphosphate). dZTP is integrated into the viral genome instead of adenine by the viral polymerase. This Z-base probably completely replaces adenosine and forms a triple bond to the opposite T-base. The resulting non-standard viral DNA is called Z-genome. The chemically modified DNA is probably harder for the host bacteria to digest with nucleases or restriction enzymes. |
Catalytic activity | RHEA:67628: ATP + dGMP + L-aspartate = (2S)-2-amino-2'-deoxyadenylo-succinate + ADP + 2 H(+) + phosphate |
Cofactor | Mg(2+) |
Pathway | Purine metabolism. |
Similarity | Belongs to the Caudovirales PurZ family. |
Pfam | PF00709; Adenylsucc_synt; 0-2; |
From: PURZ_BPVC8 (G3FFN6) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
ACT_SITE | 14 | 14 | S | |||||||||
METAL | 14 | 14 | Magnesium | S | 1 | |||||||
METAL | 42 | 42 | Magnesium; via carbonyl oxygen | G | 1 | |||||||
METAL | 263 | 263 | Magnesium | T | 1 | |||||||
BINDING | 262 | 262 | Substrate | [AT] | ||||||||
BINDING | 264 | 264 | Substrate; via carbonyl oxygen | V | ||||||||
BINDING | 269 | 269 | Substrate | R |
Size range | 320-420 amino acids |
Related rules | None |
Fusion | None |