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HAMAP rule MF_04207

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General rule information [?]

Accession MF_04207
Dates 5-APR-2018 (Created)
29-SEP-2022 (Last updated, Version 5)
Scope(s) Viruses
Template(s) P31614 (HEMA_CVMS); [ Recover all ]
Triggered by HAMAP; MF_04207 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier HEMA
Protein name RecName: Full=Hemagglutinin-esterase;
                 Short=HE protein;
AltName: Full=E3 glycoprotein;
                 Flags: Precursor;
Gene name Name=HE;

Comments [?]

FUNCTIONStructural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-4-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. May become a target for both the humoral and the cellular branches of the immune system.
CATALYTIC ACTIVITY Reaction=H2O + N-acetyl-9-O-acetylneuraminate = acetate + H(+) + N- acetylneuraminate; Xref=Rhea:RHEA:22600, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28999, ChEBI:CHEBI:30089, ChEBI:CHEBI:35418; EC=;
CATALYTIC ACTIVITY Reaction=H2O + N-acetyl-4-O-acetylneuraminate = acetate + H(+) + N- acetylneuraminate; Xref=Rhea:RHEA:25564, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29006, ChEBI:CHEBI:30089, ChEBI:CHEBI:35418; EC=;
SUBUNITHomodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.
SUBCELLULAR LOCATIONVirion membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass type I membrane protein. Note=In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface.
PTMN-glycosylated in the host RER.
SIMILARITYBelongs to the influenza type C/coronaviruses hemagglutinin-esterase family.

Keywords [?]

Gene Ontology [?]

GO:0020002; Cellular component:host cell plasma membrane
GO:0016020; Cellular component:membrane
GO:0019031; Cellular component:viral envelope
GO:0055036; Cellular component:virion membrane
GO:0046789; Molecular function:host cell surface receptor binding
GO:0001681; Molecular function:sialate O-acetylesterase activity
GO:0019064; Biological process:fusion of virus membrane with host plasma membrane

Cross-references [?]

Pfam PF03996; Hema_esterase; 1;
Pfam PF02710; Hema_HEFG; 1;
General Signal; -; 1;
General Transmembrane; -; 1;

Features [?]

From: HEMA_CVMS (P31614)
Key From To Description Tag Condition FTGroup
SIGNAL Nter 22
CHAIN 23 Cter /note="Hemagglutinin-esterase"
TOPO_DOM 23 407 /note="Virion surface"
TRANSMEM 408 428 /note="Helical"
TOPO_DOM 429 Cter /note="Intravirion"
REGION 12 132 /note="Esterase domain 1"
REGION 133 281 /note="Receptor binding"
REGION 282 395 /note="Esterase domain 2"
ACT_SITE 45 45 /note="Nucleophile" S
ACT_SITE 342 342 /note="Charge relay system" D
ACT_SITE 345 345 /note="Charge relay system" H
DISULFID 49 70 C-x*-C
DISULFID 118 167 C-x*-C
DISULFID 202 291 C-x*-C
DISULFID 210 264 C-x*-C
DISULFID 322 327 C-x*-C
DISULFID 363 387 C-x*-C
DISULFID 400 400 /note="Interchain" C-x*-C

Additional information [?]

Size range 385-439 amino acids
Related rules None
Fusion Nter: None Cter: None

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