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Annotation rule MF_04207
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General rule information [?]

Accession MF_04207
Dates 5-APR-2018 (Created)
20-NOV-2019 (Last updated, Version 4)
Viruses; Betacoronavirus
Template P31614 (HEMA_CVMS)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Hemagglutinin-esterase;
Short=HE protein;
AltName: Full=E3 glycoprotein;
Flags: Precursor;
Gene name

Comments [?]

Function Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-4-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. May become a target for both the humoral and the cellular branches of the immune system.
Catalytic activity RHEA:22600: H2O + N-acetyl-9-O-acetylneuraminate = acetate + H(+) + N-acetylneuraminate
RHEA:25564: H2O + N-acetyl-4-O-acetylneuraminate = acetate + H(+) + N-acetylneuraminate
Subunit Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.
Subcellular location Virion membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass type I membrane protein. Note=In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface.
Ptm N-glycosylated in the host RER.
Similarity Belongs to the influenza type C/coronaviruses hemagglutinin-esterase family.

Keywords [?]

Gene Ontology [?]

GO:0020002; Cellular component: host cell plasma membrane.
GO:0016021; Cellular component: integral component of membrane.
GO:0019031; Cellular component: viral envelope.
GO:0055036; Cellular component: virion membrane.
GO:0046789; Molecular function: host cell surface receptor binding.
GO:0001681; Molecular function: sialate O-acetylesterase activity.
GO:0019064; Biological process: fusion of virus membrane with host plasma membrane.

Cross-references [?]

PROSITE PS00001; ASN_GLYCOSYLATION; 0-unlimited; trigger=PRU00498;
Pfam PF03996; Hema_esterase; 1;
PF02710; Hema_HEFG; 1;

Computed features [?]

General Signal; -; 1;
Transmembrane; -; 1;

Features [?]

From: HEMA_CVMS (P31614)
Key     From     To       Description   Tag   Condition   FTGroup
SIGNAL     Nter     22                
CHAIN     23     Cter       Hemagglutinin-esterase        
TOPO_DOM     23     407       Virion surface        
TRANSMEM     408     428       Helical        
TOPO_DOM     429     Cter       Intravirion        
REGION     12     132       Esterase domain 1        
REGION     133     281       Receptor binding        
REGION     282     395       Esterase domain 2        
ACT_SITE     45     45       Nucleophile     S  
ACT_SITE     342     342       Charge relay system     D  
ACT_SITE     345     345       Charge relay system     H  
DISULFID     49     70             C-x*-C  
DISULFID     118     167             C-x*-C  
DISULFID     202     291             C-x*-C  
DISULFID     210     264             C-x*-C  
DISULFID     322     327             C-x*-C  
DISULFID     363     387             C-x*-C  
DISULFID     400     400       Interchain     C-x*-C  

Additional information [?]

Size range 385-439 amino acids
Related rules None
Fusion None