HAMAP rule MF_04207
General rule information
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| Accession | MF_04207 |
| Dates | 5-APR-2018 (Created)
29-SEP-2022 (Last updated, Version 5) |
| Name | BETA_CORONA_HE |
| Scope(s) |
Viruses Betacoronavirus |
| Template(s) | P31614 (HEMA_CVMS); [ Recover all ] |
| Triggered by |
HAMAP; MF_04207 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | HEMA |
| Protein name | RecName: Full=Hemagglutinin-esterase; Short=HE protein; EC=3.1.1.53; AltName: Full=E3 glycoprotein; Flags: Precursor; |
| Gene name | Name=HE; |
Comments
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| FUNCTION | Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-4-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. May become a target for both the humoral and the cellular branches of the immune system. |
| CATALYTIC ACTIVITY | Reaction=H2O + N-acetyl-9-O-acetylneuraminate = acetate + H(+) + N- acetylneuraminate; Xref=Rhea:RHEA:22600, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28999, ChEBI:CHEBI:30089, ChEBI:CHEBI:35418; EC=3.1.1.53; |
| CATALYTIC ACTIVITY | Reaction=H2O + N-acetyl-4-O-acetylneuraminate = acetate + H(+) + N- acetylneuraminate; Xref=Rhea:RHEA:25564, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29006, ChEBI:CHEBI:30089, ChEBI:CHEBI:35418; EC=3.1.1.53; |
| SUBUNIT | Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE. |
| SUBCELLULAR LOCATION | Virion membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass type I membrane protein. Note=In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface. |
| PTM | N-glycosylated in the host RER. |
| SIMILARITY | Belongs to the influenza type C/coronaviruses hemagglutinin-esterase family. |
Keywords
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| Disulfide bond |
| Glycoprotein |
| Hemagglutinin |
| Host cell membrane |
| Host membrane |
| Hydrolase |
| Membrane |
| Signal |
| Transmembrane |
| Transmembrane helix |
| Viral envelope protein |
| Virion |
Gene Ontology
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| GO:0020002; Cellular component:host cell plasma membrane |
| GO:0016020; Cellular component:membrane |
| GO:0019031; Cellular component:viral envelope |
| GO:0055036; Cellular component:virion membrane |
| GO:0046789; Molecular function:host cell surface receptor binding |
| GO:0001681; Molecular function:sialate O-acetylesterase activity |
| GO:0019064; Biological process:fusion of virus membrane with host plasma membrane |
Cross-references
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| PROSITE | PS00001; ASN_GLYCOSYLATION; 0-unlimited; |
| Pfam | PF03996; Hema_esterase; 1; |
| Pfam | PF02710; Hema_HEFG; 1; |
| General | Signal; -; 1; |
| General | Transmembrane; -; 1; |
Features
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| From: HEMA_CVMS (P31614) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| SIGNAL | Nter | 22 | ||||||||||
| CHAIN | 23 | Cter | /note="Hemagglutinin-esterase" | |||||||||
| TOPO_DOM | 23 | 407 | /note="Virion surface" | |||||||||
| TRANSMEM | 408 | 428 | /note="Helical" | |||||||||
| TOPO_DOM | 429 | Cter | /note="Intravirion" | |||||||||
| REGION | 12 | 132 | /note="Esterase domain 1" | |||||||||
| REGION | 133 | 281 | /note="Receptor binding" | |||||||||
| REGION | 282 | 395 | /note="Esterase domain 2" | |||||||||
| ACT_SITE | 45 | 45 | /note="Nucleophile" | S | ||||||||
| ACT_SITE | 342 | 342 | /note="Charge relay system" | D | ||||||||
| ACT_SITE | 345 | 345 | /note="Charge relay system" | H | ||||||||
| DISULFID | 49 | 70 | C-x*-C | |||||||||
| DISULFID | 118 | 167 | C-x*-C | |||||||||
| DISULFID | 202 | 291 | C-x*-C | |||||||||
| DISULFID | 210 | 264 | C-x*-C | |||||||||
| DISULFID | 322 | 327 | C-x*-C | |||||||||
| DISULFID | 363 | 387 | C-x*-C | |||||||||
| DISULFID | 400 | 400 | /note="Interchain" | C-x*-C | ||||||||
Additional information
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| Size range | 385-439 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |