HAMAP rule MF_00993
General rule information
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| Accession | MF_00993 |
| Dates | 14-FEB-2014 (Created)
1-JUN-2023 (Last updated, Version 13) |
| Name | MqnE |
| Scope(s) |
Bacteria |
| Template(s) | Q5SK48 (MQNE_THET8); [ Recover all ] |
| Triggered by |
HAMAP; MF_00993 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | MQNE |
| Protein name | RecName: Full=Aminodeoxyfutalosine synthase; Short=AFL synthase; Short=Aminofutalosine synthase; EC=2.5.1.120; AltName: Full=Menaquinone biosynthetic enzyme MqnE; |
| Gene name | Name=mqnE; |
Comments
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| FUNCTION | Radical SAM enzyme that catalyzes the addition of the adenosyl radical to the double bond of 3-[(1-carboxyvinyl)oxy]benzoate, leading to aminodeoxyfutalosine (AFL), a key intermediate in the formation of menaquinone (MK, vitamin K2) from chorismate. |
| CATALYTIC ACTIVITY | Reaction=3-[(1-carboxyvinyl)-oxy]benzoate + H2O + S-adenosyl-L- methionine = 6-amino-6-deoxyfutalosine + H(+) + hydrogencarbonate + L-methionine; Xref=Rhea:RHEA:33075, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17544, ChEBI:CHEBI:57844, ChEBI:CHEBI:59789, ChEBI:CHEBI:64286, ChEBI:CHEBI:76981; EC=2.5.1.120; |
| case <FTGroup:1> | |
| COFACTOR | Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.; |
| end case | |
| PATHWAY | Quinol/quinone metabolism; menaquinone biosynthesis. |
| SIMILARITY | Belongs to the radical SAM superfamily. MqnE family. |
Keywords
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| case <FTGroup:1> | |
| 4Fe-4S | |
| Iron | |
| Iron-sulfur | |
| Metal-binding | |
| S-adenosyl-L-methionine | |
| end case | |
| Menaquinone biosynthesis | |
| Transferase | |
Gene Ontology
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| case <FTGroup:1> | |
| GO:0005506; Molecular function:iron ion binding | |
| end case | |
| GO:0016765; Molecular function:transferase activity, transferring alkyl or aryl (other than methyl) groups | |
| GO:0009234; Biological process:menaquinone biosynthetic process | |
Cross-references
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| Pfam | PF04055; Radical_SAM; 1; |
| NCBIfam | TIGR03700; mena_SCO4494; 1; |
| NCBIfam | TIGR00423; TIGR00423; 1; |
| PIRSF | PIRSF004762; CHP00423; 1; |
| PROSITE | PS51918; RADICAL_SAM; 1; |
Features
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| From: MQNE_THET8 (Q5SK48) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 69 | 69 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet" |
C | 1 | |||||||
| BINDING | 73 | 73 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet" |
C | 1 | |||||||
| BINDING | 76 | 76 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet" |
C | 1 | |||||||
Additional information
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| Size range | 335-420 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |