HAMAP rule MF_01856
General rule information
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| Accession | MF_01856 |
| Dates | 5-FEB-2009 (Created)
1-JUN-2023 (Last updated, Version 15) |
| Name | 16SrRNA_methyltr_B |
| Scope(s) |
Bacteria Enterobacterales |
| Template(s) | P36929 (RSMB_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_01856 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | RSMB |
| Protein name | RecName: Full=Ribosomal RNA small subunit methyltransferase B; EC=2.1.1.176; AltName: Full=16S rRNA m5C967 methyltransferase; AltName: Full=rRNA (cytosine-C(5)-)-methyltransferase RsmB; |
| Gene name | Name=rsmB; Synonyms=sun; |
Comments
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| FUNCTION | Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA. |
| CATALYTIC ACTIVITY | Reaction=cytidine(967) in 16S rRNA + S-adenosyl-L-methionine = 5- methylcytidine(967) in 16S rRNA + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:42748, Rhea:RHEA-COMP:10219, Rhea:RHEA-COMP:10220, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74483, ChEBI:CHEBI:82748; EC=2.1.1.176; |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family. |
Keywords
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Gene Ontology
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| GO:0016434; Molecular function:rRNA (cytosine) methyltransferase activity |
| GO:0031167; Biological process:rRNA methylation |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| PROSITE | PS01153; NOL1_NOP2_SUN; 1; |
| Pfam | PF01189; Nol1_Nop2_Fmu; 1; |
| Pfam | PF01029; NusB; 1; |
| NCBIfam | TIGR00563; RsmB; 1; |
| PROSITE | PS01153; NOL1_NOP2_SUN; 1; |
| PROSITE | PS51686; SAM_MT_RSMB_NOP; 1; |
Features
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| From: RSMB_ECOLI (P36929) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 254 | 260 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[ACSV]-[ACPS]-[AGP]-[PQ]-[AG]-[GINS]-K | ||||||||
| ACT_SITE | 375 | 375 | /note="Nucleophile" | C | ||||||||
| BINDING | 277 | 277 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
D | ||||||||
| BINDING | 303 | 303 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
D | ||||||||
| BINDING | 322 | 322 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
D | ||||||||
Additional information
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| Size range | 422-433 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | The family is restricted to Enterobacterales in order to avoid wrong matches. |