HAMAP rule MF_01885
General rule information
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| Accession | MF_01885 |
| Dates | 21-OCT-2010 (Created)
1-JUN-2023 (Last updated, Version 12) |
| Name | tRNA_methyltr_TrmL |
| Scope(s) |
Bacteria |
| Template(s) | P0AGJ7 (TRML_ECOLI); P44868 (TRML_HAEIN); Q74Y93 (TRML_YERPE); [ Recover all ] |
| Triggered by |
HAMAP; MF_01885 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | TRML |
| case <OC:Pseudomonadota> | |
| Protein name | RecName: Full=tRNA (cytidine(34)-2'-O)-methyltransferase; EC=2.1.1.207; AltName: Full=tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmL; |
| Gene name | Name=trmL; |
| else | |
| Protein name | RecName: Full=Putative tRNA (cytidine(34)-2'-O)-methyltransferase; EC=2.1.1.207; AltName: Full=tRNA (cytidine/uridine-2'-O-)-methyltransferase; |
| end case | |
Comments
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| case <OC:Pseudomonadota> | |
| FUNCTION | Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH of the wobble nucleotide. |
| else | |
| FUNCTION | Could methylate the ribose at the nucleotide 34 wobble position in tRNA. |
| end case | |
| CATALYTIC ACTIVITY | Reaction=cytidine(34) in tRNA + S-adenosyl-L-methionine = 2'-O- methylcytidine(34) in tRNA + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:43084, Rhea:RHEA-COMP:10331, Rhea:RHEA-COMP:10332, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74495, ChEBI:CHEBI:82748; EC=2.1.1.207; |
| CATALYTIC ACTIVITY | Reaction=5-carboxymethylaminomethyluridine(34) in tRNA(Leu) + S- adenosyl-L-methionine = 5-carboxymethylaminomethyl-2'-O- methyluridine(34) in tRNA(Leu) + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:43088, Rhea:RHEA-COMP:10333, Rhea:RHEA-COMP:10334, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74508, ChEBI:CHEBI:74511; EC=2.1.1.207; |
| case <OC:Pseudomonadota> | |
| SUBUNIT | Homodimer. |
| end case | |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. TrmL subfamily. |
Keywords
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Gene Ontology
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| GO:0008175; Molecular function:tRNA methyltransferase activity |
| GO:0008757; Molecular function:S-adenosylmethionine-dependent methyltransferase activity |
| GO:0030488; Biological process:tRNA methylation |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| Pfam | PF00588; SpoU_methylase; 1; |
| NCBIfam | TIGR00185; RRNA_methyl_2; 1; |
| PIRSF | PIRSF029256; SpoU_TrmH_prd; 1; |
Features
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| From: TRML_HAEIN (P44868) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 78 | 78 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[ILVM] | ||||||||
| BINDING | 100 | 100 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
G | ||||||||
| BINDING | 122 | 122 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[ILVM] | ||||||||
| BINDING | 130 | 130 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
S | ||||||||
Additional information
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| Size range | 140-230 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |