HAMAP rule MF_00008

General rule information [?]

Accession	MF_00008
Dates	1-JUN-2001 (Created) 19-NOV-2022 (Last updated, Version 42)

Name	Thymidy_synth_bact

Scope

Bacteria

Archaea; Halobacteria

Templates

P0CI79 (TYSY1_BACSU); P0A884 (TYSY_ECOLI); P00469 (TYSY_LACCA); P9WFR9 (TYSY_MYCTU): [Recover all]

Triggered by

HAMAP; MF_00008 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

TYSY

Protein name

RecName:		Full=Thymidylate synthase;
		Short=TS;
		Short=TSase;
		EC 2.1.1.45;

Gene name

thyA

Comments [?]

Function	Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.
Catalytic activity	RHEA:12104: (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP = 7,8-dihydrofolate + dTMP EC 2.1.1.45
Pathway	Pyrimidine metabolism; dTTP biosynthesis.
Subunit	Homodimer.
Subcellular location	Cytoplasm.
Similarity	Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Keywords [?]

Cytoplasm
Methyltransferase
Nucleotide biosynthesis
Transferase

Gene Ontology [?]

GO:0004799; Molecular function: thymidylate synthase activity.
GO:0006231; Biological process: dTMP biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE	PS00091; THYMIDYLATE_SYNTHASE; 1;
Pfam	PF00303; Thymidylat_synt; 1;
PRINTS	PR00108; THYMDSNTHASE; 1;
TIGRFAMs	TIGR03284; thym_sym; 1;

Features [?]

From: TYSY_ECOLI (P0A884)

Key From To Description Tag Condition FTGroup

BINDING 126 127 /ligand="dUMP" /ligand_id="ChEBI:CHEBI:246422" /ligand_note="ligand shared between dimeric partners R-R

BINDING 166 169 /ligand="dUMP" /ligand_id="ChEBI:CHEBI:246422" /ligand_note="ligand shared between dimeric partners" /note="in other chain R-S-x-D

BINDING 207 209 /ligand="dUMP" /ligand_id="ChEBI:CHEBI:246422" /ligand_note="ligand shared between dimeric partners" /note="in other chain H-x-Y

ACT_SITE 146 146 Nucleophile C

BINDING (Optional) 21 21 /ligand="dUMP" /ligand_id="ChEBI:CHEBI:246422" /ligand_note="ligand shared between dimeric partners" /note="in other chain R

BINDING (Optional) 51 51 /ligand="(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate" /ligand_id="ChEBI:CHEBI:15636 [HN]

BINDING 169 169 /ligand="(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate" /ligand_id="ChEBI:CHEBI:15636 D

BINDING 177 177 /ligand="dUMP" /ligand_id="ChEBI:CHEBI:246422" /ligand_note="ligand shared between dimeric partners" /note="in other chain N

BINDING (Optional) 263 263 /ligand="(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate" /ligand_id="ChEBI:CHEBI:15636 [AS]

Additional information [?]

Size range	258-323 amino acids
Related rules	MF_01686 (TYSY supersedes the current rule)
Fusion	None
Comments	Duplicates in Bacillus subtilis or Bacillus amyloliquefaciens are due to the presence of a phage-derived TS. See: PubMed=9648749. Tam N.H., Borriss R.; "Genes encoding thymidylate synthases A and B in the genus Bacillus are members of two distinct families."; Mol. Gen. Genet. 258:427-430(1998). Archaeal thyA belong to a separate family (MF_01686)

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