HAMAP rule MF_00008
General rule information
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| Accession | MF_00008 |
| Dates | 1-JUN-2001 (Created)
31-JAN-2024 (Last updated, Version 44) |
| Name | Thymidy_synth_bact |
| Scope(s) |
Bacteria Archaea Halobacteria |
| Template(s) | P0CI79 (TYSY1_BACSU); P0A884 (TYSY_ECOLI); P00469 (TYSY_LACCA); P9WFR9 (TYSY_MYCTU); [ Recover all ] |
| Triggered by |
HAMAP; MF_00008 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | TYSY |
| Protein name | RecName: Full=Thymidylate synthase; Short=TS; Short=TSase; EC=2.1.1.45; |
| Gene name | Name=thyA; |
Comments
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| FUNCTION | Catalyzes the reductive methylation of 2'-deoxyuridine-5'- monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by- product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis. |
| CATALYTIC ACTIVITY | Reaction=(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP = 7,8- dihydrofolate + dTMP; Xref=Rhea:RHEA:12104, ChEBI:CHEBI:15636, ChEBI:CHEBI:57451, ChEBI:CHEBI:63528, ChEBI:CHEBI:246422; EC=2.1.1.45; |
| PATHWAY | Pyrimidine metabolism; dTTP biosynthesis. |
| SUBUNIT | Homodimer. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily. |
Keywords
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Gene Ontology
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| GO:0004799; Molecular function:thymidylate synthase activity |
| GO:0006231; Biological process:dTMP biosynthetic process |
| GO:0006235; Biological process:dTTP biosynthetic process |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| PROSITE | PS00091; THYMIDYLATE_SYNTHASE; 1; |
| Pfam | PF00303; Thymidylat_synt; 1; |
| PRINTS | PR00108; THYMDSNTHASE; 1; |
| NCBIfam | TIGR03284; thym_sym; 1; |
Features
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| From: TYSY_ECOLI (P0A884) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 126 | 127 | /ligand="dUMP" /ligand_id="ChEBI:CHEBI:246422" /ligand_note="ligand shared between dimeric partners" |
R-R | ||||||||
| BINDING | 166 | 169 | /ligand="dUMP" /ligand_id="ChEBI:CHEBI:246422" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
R-S-x-D | ||||||||
| BINDING | 207 | 209 | /ligand="dUMP" /ligand_id="ChEBI:CHEBI:246422" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
H-x-Y | ||||||||
| ACT_SITE | 146 | 146 | /note="Nucleophile" | C | ||||||||
| BINDING | 21 | 21 | /ligand="dUMP" /ligand_id="ChEBI:CHEBI:246422" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
R | ||||||||
| BINDING | 51 | 51 | /ligand="(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate" /ligand_id="ChEBI:CHEBI:15636" |
[HN] | ||||||||
| BINDING | 169 | 169 | /ligand="(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate" /ligand_id="ChEBI:CHEBI:15636" |
D | ||||||||
| BINDING | 177 | 177 | /ligand="dUMP" /ligand_id="ChEBI:CHEBI:246422" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
N | ||||||||
| BINDING | 263 | 263 | /ligand="(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate" /ligand_id="ChEBI:CHEBI:15636" |
[AS] | ||||||||
Additional information
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| Size range | 258-323 amino acids |
| Related rules |
MF_01686 |
| Fusion | Nter: None Cter: None |
| Comments | Duplicates in Bacillus subtilis or Bacillus amyloliquefaciens are due to the presence of a phage-derived TS. See: PubMed=9648749. Tam N.H., Borriss R.; "Genes encoding thymidylate synthases A and B in the genus Bacillus are members of two distinct families."; Mol. Gen. Genet. 258:427-430(1998). Archaeal thyA belong to a separate family (MF_01686) |