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Annotation rule MF_00011 |
General rule information
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Accession |
MF_00011 |
Dates |
1-JUN-2001 (Created) 20-NOV-2019 (Last updated, Version 49) |
case <OC:Bacteria> or <OC:Archaea>
end case
Propagated annotation
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Identifier, protein and gene names
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Protein name |
RecName:
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Full=Adenylosuccinate synthetase;
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Short=AMPSase;
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Short=AdSS;
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EC 6.3.4.4;
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AltName:
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Full=IMP--aspartate ligase;
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Function |
Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP. |
Catalytic activity |
RHEA:15753: GTP + IMP + L-aspartate = GDP + 2 H(+) + N(6)-(1,2-dicarboxyethyl)-AMP + phosphate
EC 6.3.4.4
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Cofactor |
Mg(2+) Note: Binds 1 Mg(2+) ion per subunit. |
Pathway |
Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. |
Subunit |
Homodimer. |
Subcellular location |
Cytoplasm. |
Similarity |
Belongs to the adenylosuccinate synthetase family. |
GO:0004019; Molecular function: adenylosuccinate synthase activity.
GO:0005525; Molecular function: GTP binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0006164; Biological process: purine nucleotide biosynthetic process.
GO:0005737; Cellular component: cytoplasm.
From: PURA_ECOLI (P0A7D4) |
Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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NP_BIND
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13
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19
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GTP
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G-D-E-[GA]-K-[GA]-x
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NP_BIND
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41
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43
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GTP
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G-H-x
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NP_BIND
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332
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334
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GTP
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x-x-D
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NP_BIND
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415
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417
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GTP
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x-x-[GS]
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REGION
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14
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17
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IMP binding
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D-E-[GA]-K
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REGION
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39
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42
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IMP binding
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N-[AS]-G-H
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REGION
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300
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306
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Substrate binding
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x-x-[ST]-x-[KR]-x-R
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ACT_SITE
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14
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14
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Proton acceptor
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D
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ACT_SITE
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42
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42
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Proton donor
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H
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METAL
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14
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14
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Magnesium
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D
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METAL
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41
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41
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Magnesium; via carbonyl oxygen
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G
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BINDING
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130
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130
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IMP
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T
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BINDING
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144
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144
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IMP; shared with dimeric partner
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[KR]
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BINDING
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225
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225
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IMP
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[QN]
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BINDING
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240
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240
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IMP
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T
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BINDING
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304
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304
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IMP
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[KR]
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BINDING
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306
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306
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GTP
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R
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Additional information
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Size range |
336-459 amino acids |
Related rules |
MF_03126 (PURA1 supersedes the current rule); MF_03127 (PURA2 supersedes the current rule) |
Fusion |
None |