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Annotation rule MF_00011
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General rule information [?]

Accession MF_00011
Dates 1-JUN-2001 (Created)
20-NOV-2019 (Last updated, Version 49)
Name Adenylosucc_synth
Scope
Bacteria
Archaea
Template P0A7D4 (PURA_ECOLI)
case <OC:Bacteria> or <OC:Archaea>
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
PURA
Protein name
RecName: Full=Adenylosuccinate synthetase;
Short=AMPSase;
Short=AdSS;
EC 6.3.4.4;
AltName: Full=IMP--aspartate ligase;
Gene name
purA

Comments [?]

Function Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.
Catalytic activity RHEA:15753: GTP + IMP + L-aspartate = GDP + 2 H(+) + N(6)-(1,2-dicarboxyethyl)-AMP + phosphate
EC 6.3.4.4
Cofactor Mg(2+)
Note: Binds 1 Mg(2+) ion per subunit.
Pathway Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.
Subunit Homodimer.
Subcellular location Cytoplasm.
Similarity Belongs to the adenylosuccinate synthetase family.

Keywords [?]


Gene Ontology [?]

GO:0004019; Molecular function: adenylosuccinate synthase activity.
GO:0005525; Molecular function: GTP binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0006164; Biological process: purine nucleotide biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00709; Adenylsucc_synt; 1;
TIGRFAMs TIGR00184; purA; 1;
PROSITE PS01266; ADENYLOSUCCIN_SYN_1; 1;
PS00513; ADENYLOSUCCIN_SYN_2; 1;

Features [?]

From: PURA_ECOLI (P0A7D4)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     13     19       GTP     G-D-E-[GA]-K-[GA]-x  
NP_BIND     41     43       GTP     G-H-x  
NP_BIND     332     334       GTP     x-x-D  
NP_BIND     415     417       GTP     x-x-[GS]  
REGION     14     17       IMP binding     D-E-[GA]-K  
REGION     39     42       IMP binding     N-[AS]-G-H  
REGION     300     306       Substrate binding     x-x-[ST]-x-[KR]-x-R  
ACT_SITE     14     14       Proton acceptor     D  
ACT_SITE     42     42       Proton donor     H  
METAL     14     14       Magnesium     D  
METAL     41     41       Magnesium; via carbonyl oxygen     G  
BINDING     130     130       IMP     T  
BINDING     144     144       IMP; shared with dimeric partner     [KR]  
BINDING     225     225       IMP     [QN]  
BINDING     240     240       IMP     T  
BINDING     304     304       IMP     [KR]  
BINDING     306     306       GTP     R  

Additional information [?]

Size range 336-459 amino acids
Related rules MF_03126 (PURA1 supersedes the current rule); MF_03127 (PURA2 supersedes the current rule)
Fusion None