 |
|
| Annotation rule MF_00012 |
General rule information
[?]
| Accession | MF_00012 |
| Dates | 1-JUN-2001 (Created) 19-NOV-2022 (Last updated, Version 38) |
| Name | IlvD |
| Scope | Bacteria
Archaea |
| Templates | P05791 (ILVD_ECOLI); P9WKJ5 (ILVD_MYCTU): [Recover all] |
| Triggered by |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
| Identifier |
|
| Protein name |
|
||||||||||||
| Gene name |
|
Comments
[?]
| Function | Functions in the biosynthesis of branched-chain amino acids. Catalyzes the dehydration of (2R,3R)-2,3-dihydroxy-3-methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2-oxo-3-methylpentanoate (2-oxo-3-methylvalerate) and of (2R)-2,3-dihydroxy-3-methylbutanoate (2,3-dihydroxyisovalerate) into 2-oxo-3-methylbutanoate (2-oxoisovalerate), the penultimate precursor to L-isoleucine and L-valine, respectively. |
| Catalytic activity | RHEA:24809: (2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O
EC 4.2.1.9 PhysiologicalDirection=left-to-right (RHEA:24810) |
| RHEA:27694: (2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2-oxopentanoate + H2O
EC 4.2.1.9 PhysiologicalDirection=left-to-right (RHEA:27695) |
|
| Cofactor | [2Fe-2S] cluster Note: Binds 1 [2Fe-2S] cluster per subunit. This cluster acts as a Lewis acid cofactor |
| Mg(2+) | |
| Pathway | Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. |
| Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. | |
| Subunit | Homodimer. |
| Similarity | Belongs to the IlvD/Edd family. |
Keywords
[?]
Amino-acid biosynthesis
Branched-chain amino acid biosynthesis
Lyase
Metal-binding
Iron
Iron-sulfur
2Fe-2S
Magnesium
Branched-chain amino acid biosynthesis
Lyase
Metal-binding
Iron
Iron-sulfur
2Fe-2S
Magnesium
Gene Ontology
[?]
GO:0000287; Molecular function: magnesium ion binding.
GO:0004160; Molecular function: dihydroxy-acid dehydratase activity.
GO:0051537; Molecular function: 2 iron, 2 sulfur cluster binding.
GO:0009097; Biological process: isoleucine biosynthetic process.
GO:0009099; Biological process: valine biosynthetic process.
GO:0004160; Molecular function: dihydroxy-acid dehydratase activity.
GO:0051537; Molecular function: 2 iron, 2 sulfur cluster binding.
GO:0009097; Biological process: isoleucine biosynthetic process.
GO:0009099; Biological process: valine biosynthetic process.
Cross-references
[?]
| Pfam | PF00920; ILVD_EDD; 1; |
| TIGRFAMs | TIGR00110; IlvD; 1; |
| PROSITE | PS00886; ILVD_EDD_1; 1; |
| PS00887; ILVD_EDD_2; 1; |
Features
[?]
| From: ILVD_MYCTU (P9WKJ5) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 491 | 491 | Proton acceptor | S | ||||||||
| BINDING (Optional) | 64 | 64 | /ligand="[2Fe-2S] cluster" /ligand_id="ChEBI:CHEBI:190135 | C | ||||||||
| BINDING | 96 | 96 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 | D | ||||||||
| BINDING | 122 | 122 | /ligand="[2Fe-2S] cluster" /ligand_id="ChEBI:CHEBI:190135 | C | ||||||||
| BINDING | 138 | 138 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 | D | ||||||||
| BINDING | 139 | 139 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /note="via carbamate group | K | ||||||||
| BINDING | 195 | 195 | /ligand="[2Fe-2S] cluster" /ligand_id="ChEBI:CHEBI:190135 | C | ||||||||
| BINDING | 465 | 465 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 | E | ||||||||
| MOD_RES | 139 | 139 | N6-carboxylysine | K | ||||||||
Additional information
[?]
| Size range | 547-629 amino acids |
| Related rules | None |
| Fusion | None |