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Annotation rule MF_00012
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General rule information [?]

Accession MF_00012
Dates 1-JUN-2001 (Created)
14-MAY-2022 (Last updated, Version 37)
Name IlvD
Scope
Bacteria
Archaea
Templates P05791 (ILVD_ECOLI); P9WKJ5 (ILVD_MYCTU): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
ILVD
Protein name
RecName: Full=Dihydroxy-acid dehydratase;
Short=DAD;
EC 4.2.1.9;
Gene name
ilvD

Comments [?]

Function Functions in the biosynthesis of branched-chain amino acids. Catalyzes the dehydration of (2R,3R)-2,3-dihydroxy-3-methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2-oxo-3-methylpentanoate (2-oxo-3-methylvalerate) and of (2R)-2,3-dihydroxy-3-methylbutanoate (2,3-dihydroxyisovalerate) into 2-oxo-3-methylbutanoate (2-oxoisovalerate), the penultimate precursor to L-isoleucine and L-valine, respectively.
Catalytic activity RHEA:24809: (2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O
EC 4.2.1.9
PhysiologicalDirection=left-to-right (RHEA:24810)
RHEA:27694: (2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2-oxopentanoate + H2O
EC 4.2.1.9
PhysiologicalDirection=left-to-right (RHEA:27695)
Cofactor [2Fe-2S] cluster
Note: Binds 1 [2Fe-2S] cluster per subunit. This cluster acts as a Lewis acid cofactor
Mg(2+)
Pathway Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4.
Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4.
Subunit Homodimer.
Similarity Belongs to the IlvD/Edd family.

Keywords [?]


Gene Ontology [?]

GO:0000287; Molecular function: magnesium ion binding.
GO:0004160; Molecular function: dihydroxy-acid dehydratase activity.
GO:0051537; Molecular function: 2 iron, 2 sulfur cluster binding.
GO:0009097; Biological process: isoleucine biosynthetic process.
GO:0009099; Biological process: valine biosynthetic process.

Cross-references [?]

Pfam PF00920; ILVD_EDD; 1;
TIGRFAMs TIGR00110; IlvD; 1;
PROSITE PS00886; ILVD_EDD_1; 1;
PS00887; ILVD_EDD_2; 1;

Features [?]

From: ILVD_MYCTU (P9WKJ5)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     491     491       Proton acceptor     S  
BINDING (Optional)     64     64       /ligand="[2Fe-2S] cluster" /ligand_id="190135     C  
BINDING     96     96       /ligand="Mg(2+)" /ligand_id="18420     D  
BINDING     122     122       /ligand="[2Fe-2S] cluster" /ligand_id="190135     C  
BINDING     138     138       /ligand="Mg(2+)" /ligand_id="18420     D  
BINDING     139     139       /ligand="Mg(2+)" /ligand_id="18420" /note="via carbamate group     K  
BINDING     195     195       /ligand="[2Fe-2S] cluster" /ligand_id="190135     C  
BINDING     465     465       /ligand="Mg(2+)" /ligand_id="18420     E  
MOD_RES     139     139       N6-carboxylysine     K  

Additional information [?]

Size range 547-629 amino acids
Related rules None
Fusion None