HAMAP rule MF_00012

General rule information [?]

Accession	MF_00012
Dates	1-JUN-2001 (Created) 1-JUN-2023 (Last updated, Version 39)

Name

IlvD

Scope

Bacteria

Archaea

Templates

P05791 (ILVD_ECOLI); P9WKJ5 (ILVD_MYCTU): [Recover all]

Triggered by

HAMAP; MF_00012 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

ILVD

Protein name

RecName:		Full=Dihydroxy-acid dehydratase;
		Short=DAD;
		EC 4.2.1.9;

Gene name

ilvD

Comments [?]

Function	Functions in the biosynthesis of branched-chain amino acids. Catalyzes the dehydration of (2R,3R)-2,3-dihydroxy-3-methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2-oxo-3-methylpentanoate (2-oxo-3-methylvalerate) and of (2R)-2,3-dihydroxy-3-methylbutanoate (2,3-dihydroxyisovalerate) into 2-oxo-3-methylbutanoate (2-oxoisovalerate), the penultimate precursor to L-isoleucine and L-valine, respectively.
Catalytic activity	RHEA:24809: (2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O EC 4.2.1.9 PhysiologicalDirection=left-to-right (RHEA:24810)
	RHEA:27694: (2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2-oxopentanoate + H2O EC 4.2.1.9 PhysiologicalDirection=left-to-right (RHEA:27695)
Cofactor	[2Fe-2S] cluster Note: Binds 1 [2Fe-2S] cluster per subunit. This cluster acts as a Lewis acid cofactor
	Mg(2+)
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4.
	Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4.
Subunit	Homodimer.
Similarity	Belongs to the IlvD/Edd family.

Keywords [?]

Amino-acid biosynthesis
Branched-chain amino acid biosynthesis
Lyase
Metal-binding
Iron
Iron-sulfur
2Fe-2S
Magnesium

Gene Ontology [?]

GO:0000287; Molecular function: magnesium ion binding.
GO:0004160; Molecular function: dihydroxy-acid dehydratase activity.
GO:0051537; Molecular function: 2 iron, 2 sulfur cluster binding.
GO:0009097; Biological process: isoleucine biosynthetic process.
GO:0009099; Biological process: valine biosynthetic process.

Cross-references [?]

Pfam	PF00920; ILVD_EDD; 1;
NCBIfam	TIGR00110; IlvD; 1;
PROSITE	PS00886; ILVD_EDD_1; 1;
	PS00887; ILVD_EDD_2; 1;

Features [?]

From: ILVD_MYCTU (P9WKJ5)

Key From To Description Tag Condition FTGroup

ACT_SITE 491 491 Proton acceptor S

BINDING (Optional) 64 64 /ligand="[2Fe-2S] cluster" /ligand_id="ChEBI:CHEBI:190135 C

BINDING 96 96 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 D

BINDING 122 122 /ligand="[2Fe-2S] cluster" /ligand_id="ChEBI:CHEBI:190135 C

BINDING 138 138 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 D

BINDING 139 139 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /note="via carbamate group K

BINDING 195 195 /ligand="[2Fe-2S] cluster" /ligand_id="ChEBI:CHEBI:190135 C

BINDING 465 465 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 E

MOD_RES 139 139 N6-carboxylysine K

Additional information [?]

Size range	547-629 amino acids
Related rules	None
Fusion	None

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