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Annotation rule MF_00013
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General rule information [?]

Accession MF_00013
Dates 1-JUN-2001 (Created)
20-NOV-2019 (Last updated, Version 41)
Name LipB
Scope
Bacteria
Archaea
Plastid
Templates P60720 (LIPB_ECOLI); P9WK83 (LIPB_MYCTU): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
LIPB
case <OC:Bacteria>
Protein name
RecName: Full=Octanoyltransferase;
EC 2.3.1.181;
AltName: Full=Lipoate-protein ligase B;
AltName: Full=Lipoyl/octanoyl transferase;
AltName: Full=Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase;
else
Protein name
RecName: Full=Probable octanoyltransferase;
EC 2.3.1.181;
AltName: Full=Lipoate-protein ligase B;
AltName: Full=Lipoyl/octanoyl transferase;
AltName: Full=Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase;
end case
Gene name
lipB

Comments [?]

Function Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate.
Catalytic activity RHEA:17665: L-lysyl-[protein] + octanoyl-[ACP] = H(+) + holo-[ACP] + N(6)-octanoyl-L-lysyl-[protein]
EC 2.3.1.181
Pathway Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 1/2.
case <OC:Bacteria> or <OC:Archaea>
Subcellular location Cytoplasm.
end case
case <OG:Chloroplast>
Subcellular location Plastid, chloroplast.
end case
Miscellaneous In the reaction, the free carboxyl group of octanoic acid is attached via an amide linkage to the epsilon-amino group of a specific lysine residue of lipoyl domains of lipoate-dependent enzymes.
Similarity Belongs to the LipB family.

Keywords [?]

case <OC:Bacteria> or <OC:Archaea>
Cytoplasm
end case
Transferase
Acyltransferase

Gene Ontology [?]

GO:0016746; Molecular function: transferase activity, transferring acyl groups.
case <OG:Chloroplast>
GO:0009507; Cellular component: chloroplast.
end case
case <OC:Bacteria> or <OC:Archaea>
GO:0005737; Cellular component: cytoplasm.
end case

Cross-references [?]

PROSITE PS51733; BPL_LPL_CATALYTIC; 1; trigger=PRU01067;
PS01313; LIPB; 1;
Pfam PF03099; BPL_LplA_LipB; 1;
TIGRFAMs TIGR00214; LipB; 1;

Features [?]

From: LIPB_MYCTU (P9WK83)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     76     83       Substrate binding     R-G-G-x(2)-[TS]-x-H  
REGION     145     147       Substrate binding     [AS]-x-[GA]  
REGION     158     160       Substrate binding     G-x-[ASG]  
ACT_SITE     176     176       Acyl-thioester intermediate     C  
SITE     142     142       Lowers pKa of active site Cys     K  

Additional information [?]

Size range 180-330 amino acids
Related rules None
Fusion Nter: <Unknown>; Cter: <Nudix>
Comments Unknown N-terminal domains in Deinococcus radiodurans and Porphyromonas gingivalis. Nudix-like C-terminal domain in Myxococcus xanthus.


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