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Annotation rule MF_00021 |
General rule information
[?]
Accession |
MF_00021 |
Dates |
1-JUN-2001 (Created) 20-NOV-2019 (Last updated, Version 40) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
case <Feature:PS50206>
Protein name |
RecName:
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Full=tRNA sulfurtransferase;
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EC 2.8.1.4;
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AltName:
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Full=Sulfur carrier protein ThiS sulfurtransferase;
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AltName:
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Full=Thiamine biosynthesis protein ThiI;
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AltName:
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Full=tRNA 4-thiouridine synthase;
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end case
case not <Feature:PS50206>
Protein name |
RecName:
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Full=Probable tRNA sulfurtransferase;
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EC 2.8.1.4;
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AltName:
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Full=Sulfur carrier protein ThiS sulfurtransferase;
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AltName:
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Full=Thiamine biosynthesis protein ThiI;
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AltName:
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Full=tRNA 4-thiouridine synthase;
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end case
Function |
Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS. |
Catalytic activity |
RHEA:24176: [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + a uridine in tRNA + ATP + H(+) + 2 reduced [2Fe-2S]-[ferredoxin] = [ThiI sulfur-carrier protein]-L-cysteine + a 4-thiouridine in tRNA + AMP + diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin]
EC 2.8.1.4
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RHEA:43340: [sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + AH2 + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] = [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H(+) + L-cysteinyl-[cysteine desulfurase]
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Pathway |
Cofactor biosynthesis; thiamine diphosphate biosynthesis. |
Subcellular location |
Cytoplasm. |
Similarity |
Belongs to the ThiI family. |
case <Feature:PS51165>
end case
case <FT:7>
end case
case <Feature:PS51165>
end case
GO:0005524; Molecular function: ATP binding.
GO:0016783; Molecular function: sulfurtransferase activity.
GO:0009228; Biological process: thiamine biosynthetic process.
GO:0034227; Biological process: tRNA thio-modification.
GO:0005737; Cellular component: cytoplasm.
From: THII_BACAN (Q81KU0) |
Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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NP_BIND
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183
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|
184
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ATP
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[LM]-x
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NP_BIND (Optional)
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208
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209
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ATP
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[HYNTS]-[FY]
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BINDING
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265
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265
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ATP
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[RK]
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BINDING
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287
|
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287
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ATP; via amide nitrogen
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G
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BINDING
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296
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296
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ATP
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Q
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case <Feature:PS50206>
From: THII_ECOLI (P77718) |
ACT_SITE
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456
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456
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Cysteine persulfide intermediate
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C
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DISULFID
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344
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456
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Redox-active
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C-x*-C
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end case
Additional information
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Size range |
368-486 amino acids |
Related rules |
None |
Fusion |
None |
Comments |
Some proteins of this family do not possess a rhodanese domain, so there must be an external rhodanese-like partner involved. It is not known, and the reaction has not been characterized in this case. |