HAMAP rule MF_00021
General rule information
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| Accession | MF_00021 |
| Dates | 1-JUN-2001 (Created)
1-MAR-2024 (Last updated, Version 44) |
| Name | ThiI |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P77718 (THII_ECOLI); P55913 (THII_SALTY); Q81KU0 (THII_BACAN); [ Recover all ] |
| Triggered by |
HAMAP; MF_00021 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | THII |
| case <Feature:PS50206> | |
| Protein name | RecName: Full=tRNA sulfurtransferase; EC=2.8.1.4; AltName: Full=Sulfur carrier protein ThiS sulfurtransferase; AltName: Full=Thiamine biosynthesis protein ThiI; AltName: Full=tRNA 4-thiouridine synthase; |
| end case | |
| case not <Feature:PS50206> | |
| Protein name | RecName: Full=Probable tRNA sulfurtransferase; EC=2.8.1.4; AltName: Full=Sulfur carrier protein ThiS sulfurtransferase; AltName: Full=Thiamine biosynthesis protein ThiI; AltName: Full=tRNA 4-thiouridine synthase; |
| end case | |
| Gene name | Name=thiI; |
Comments
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| FUNCTION | Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS. |
| CATALYTIC ACTIVITY | Reaction=[ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + a uridine in tRNA + ATP + H(+) + 2 reduced [2Fe-2S]-[ferredoxin] = [ThiI sulfur-carrier protein]-L-cysteine + a 4-thiouridine in tRNA + AMP + diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin]; Xref=Rhea:RHEA:24176, Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001, Rhea:RHEA-COMP:13337, Rhea:RHEA-COMP:13338, Rhea:RHEA-COMP:13339, Rhea:RHEA-COMP:13340, ChEBI:CHEBI:15378, ChEBI:CHEBI:29950, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:61963, ChEBI:CHEBI:65315, ChEBI:CHEBI:136798, ChEBI:CHEBI:456215; EC=2.8.1.4; |
| CATALYTIC ACTIVITY | Reaction=[sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + AH2 + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] = [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H(+) + L- cysteinyl-[cysteine desulfurase]; Xref=Rhea:RHEA:43340, Rhea:RHEA- COMP:12157, Rhea:RHEA-COMP:12158, Rhea:RHEA-COMP:12908, Rhea:RHEA- COMP:12910, ChEBI:CHEBI:13193, ChEBI:CHEBI:15378, ChEBI:CHEBI:17499, ChEBI:CHEBI:29950, ChEBI:CHEBI:61963, ChEBI:CHEBI:90618, ChEBI:CHEBI:90619, ChEBI:CHEBI:456215; |
| PATHWAY | Cofactor biosynthesis; thiamine diphosphate biosynthesis. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the ThiI family. |
Keywords
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| case <Feature:PS51165> | |
| RNA-binding | |
| tRNA-binding | |
| end case | |
| ATP-binding | |
| Cytoplasm | |
| case <FT:7> | |
| Disulfide bond | |
| Redox-active center | |
| end case | |
| Nucleotide-binding | |
| Thiamine biosynthesis | |
| Transferase | |
Gene Ontology
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| case <Feature:PS51165> | |
| GO:0000049; Molecular function:tRNA binding | |
| end case | |
| GO:0005524; Molecular function:ATP binding | |
| GO:0016783; Molecular function:sulfurtransferase activity | |
| GO:0009229; Biological process:thiamine diphosphate biosynthetic process | |
| GO:0034227; Biological process:tRNA thio-modification | |
| GO:0005737; Cellular component:cytoplasm | |
Cross-references
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| PROSITE | PS50206; RHODANESE_3; 0-1; |
| PROSITE | PS51165; THUMP; 1; |
| Pfam | PF02568; ThiI; 1; |
| Pfam | PF02926; THUMP; 1; |
| Pfam | PF00581; Rhodanese; 1; |
| NCBIfam | TIGR04271; ThiI_C_thiazole; 0-1; |
| NCBIfam | TIGR00342; TIGR00342; 1; |
Features
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| From: THII_BACAN (Q81KU0) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 183 | 184 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[LM]-x | ||||||||
| BINDING | 208 | 209 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[HYNTS]-[FY] | ||||||||
| BINDING | 265 | 265 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[RK] | ||||||||
| BINDING | 287 | 287 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G | ||||||||
| BINDING | 296 | 296 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
Q | ||||||||
| From: THII_ECOLI (P77718) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| case <Feature:PS50206> | ||||||||||||
| ACT_SITE | 456 | 456 | /note="Cysteine persulfide intermediate" | C | ||||||||
| DISULFID | 344 | 456 | /note="Redox-active" | C-x*-C | ||||||||
| end case | ||||||||||||
Additional information
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| Size range | 368-486 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | Some proteins of this family do not possess a rhodanese domain, so there must be an external rhodanese-like partner involved. It is not known, and the reaction has not been characterized in this case. |