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HAMAP rule MF_00021

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General rule information [?]

Accession	MF_00021
Dates	1-JUN-2001 (Created) 1-MAR-2024 (Last updated, Version 44)
Name	ThiI
Scope(s)	Bacteria Archaea
Template(s)	P77718 (THII_ECOLI); P55913 (THII_SALTY); Q81KU0 (THII_BACAN); [ Recover all ]
Triggered by	HAMAP; MF_00021 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	THII
case <Feature:PS50206>
Protein name	RecName: Full=tRNA sulfurtransferase; EC=2.8.1.4; AltName: Full=Sulfur carrier protein ThiS sulfurtransferase; AltName: Full=Thiamine biosynthesis protein ThiI; AltName: Full=tRNA 4-thiouridine synthase;
end case
case not <Feature:PS50206>
Protein name	RecName: Full=Probable tRNA sulfurtransferase; EC=2.8.1.4; AltName: Full=Sulfur carrier protein ThiS sulfurtransferase; AltName: Full=Thiamine biosynthesis protein ThiI; AltName: Full=tRNA 4-thiouridine synthase;
end case
Gene name	Name=thiI;

Comments [?]

FUNCTION	Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.
CATALYTIC ACTIVITY	Reaction=[ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + a uridine in tRNA + ATP + H(+) + 2 reduced [2Fe-2S]-[ferredoxin] = [ThiI sulfur-carrier protein]-L-cysteine + a 4-thiouridine in tRNA + AMP + diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin]; Xref=Rhea:RHEA:24176, Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001, Rhea:RHEA-COMP:13337, Rhea:RHEA-COMP:13338, Rhea:RHEA-COMP:13339, Rhea:RHEA-COMP:13340, ChEBI:CHEBI:15378, ChEBI:CHEBI:29950, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:61963, ChEBI:CHEBI:65315, ChEBI:CHEBI:136798, ChEBI:CHEBI:456215; EC=2.8.1.4;
CATALYTIC ACTIVITY	Reaction=[sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + AH2 + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] = [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H(+) + L- cysteinyl-[cysteine desulfurase]; Xref=Rhea:RHEA:43340, Rhea:RHEA- COMP:12157, Rhea:RHEA-COMP:12158, Rhea:RHEA-COMP:12908, Rhea:RHEA- COMP:12910, ChEBI:CHEBI:13193, ChEBI:CHEBI:15378, ChEBI:CHEBI:17499, ChEBI:CHEBI:29950, ChEBI:CHEBI:61963, ChEBI:CHEBI:90618, ChEBI:CHEBI:90619, ChEBI:CHEBI:456215;
PATHWAY	Cofactor biosynthesis; thiamine diphosphate biosynthesis.
SUBCELLULAR LOCATION	Cytoplasm.
SIMILARITY	Belongs to the ThiI family.

Keywords [?]

case <Feature:PS51165>
RNA-binding
tRNA-binding
end case
ATP-binding
Cytoplasm
case <FT:7>
Disulfide bond
Redox-active center
end case
Nucleotide-binding
Thiamine biosynthesis
Transferase

Gene Ontology [?]

case <Feature:PS51165>
GO:0000049; Molecular function:tRNA binding
end case
GO:0005524; Molecular function:ATP binding
GO:0016783; Molecular function:sulfurtransferase activity
GO:0009229; Biological process:thiamine diphosphate biosynthetic process
GO:0034227; Biological process:tRNA thio-modification
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

PROSITE	PS50206; RHODANESE_3; 0-1;
PROSITE	PS51165; THUMP; 1;
Pfam	PF02568; ThiI; 1;
Pfam	PF02926; THUMP; 1;
Pfam	PF00581; Rhodanese; 1;
NCBIfam	TIGR04271; ThiI_C_thiazole; 0-1;
NCBIfam	TIGR00342; TIGR00342; 1;

Features [?]

From: THII_BACAN (Q81KU0)

Key

From

Description

Tag

Condition

FTGroup

BINDING

183

184