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Annotation rule MF_00022
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General rule information [?]

Accession MF_00022
Dates 1-JUN-2001 (Created)
20-NOV-2019 (Last updated, Version 47)
Name Glu_tRNA_synth_type1
Scope
Bacteria
Templates P04805 (SYE_ECOLI); O25360 (SYE2_HELPY); P27000 (SYE_THET8); Q8DLI5 (SYE_THEEB): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
SYE
Protein name
RecName: Full=Glutamate--tRNA ligase;
EC 6.1.1.17;
AltName: Full=Glutamyl-tRNA synthetase;
Short=GluRS;
Gene name
gltX

Comments [?]

Function Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).
Catalytic activity RHEA:23540: ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)
EC 6.1.1.17
case <FTGroup:1>
Cofactor Zn(2+)
Note: Binds 1 zinc ion per subunit.
end case
Subunit Monomer.
Subcellular location Cytoplasm.
Similarity Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.

Keywords [?]

case <FTGroup:1>
end case

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0004818; Molecular function: glutamate-tRNA ligase activity.
case <FTGroup:1>
GO:0008270; Molecular function: zinc ion binding.
end case
GO:0006424; Biological process: glutamyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00749; tRNA-synt_1c; 1;
PRINTS PR00987; TRNASYNTHGLU; 1;
TIGRFAMs TIGR00464; GltX_bact; 1;
PROSITE PS00178; AA_TRNA_LIGASE_I; 1;

Features [?]

From: SYE_ECOLI (P04805)
Key     From     To       Description   Tag   Condition   FTGroup
MOTIF     9     19       'HIGH' region     P-[SN]-P-x(4)-[HR]-[LIVMAT]-[GE]-x  
MOTIF     237     241       'KMSKS' region     x-[LIMSF]-S-K-[KR]  
METAL (Optional)     98     98       Zinc     C   1
METAL (Optional)     100     100       Zinc     C   1
METAL (Optional)     125     125       Zinc     [CH]   1
METAL (Optional)     127     127       Zinc     [HED]   1
BINDING     240     240       ATP     K  

Additional information [?]

Size range 431-546 amino acids
Related rules MF_01428 (GLUQ)
Fusion Nter: None; Cter: <Unknown>
Comments This signature is specific for bacterial and eukaryotic mitochondrial glutamate--tRNA ligases. See MF_02076 for archaeal and eukaryotic cytoplasmic glutamate--tRNA ligases.