HAMAP rule MF_00022

General rule information [?]

Accession	MF_00022
Dates	1-JUN-2001 (Created) 1-JUN-2023 (Last updated, Version 50)

Name	Glu_tRNA_synth_type1

Scope

Bacteria

Templates

P04805 (SYE_ECOLI); O25360 (SYE2_HELPY); P27000 (SYE_THET8); Q8DLI5 (SYE_THEVB): [Recover all]

Triggered by

HAMAP; MF_00022 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

SYE

Protein name

RecName:		Full=Glutamate--tRNA ligase;
		EC 6.1.1.17;
AltName:		Full=Glutamyl-tRNA synthetase;
		Short=GluRS;

Gene name

gltX

Comments [?]

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).
Catalytic activity	RHEA:23540: ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu) EC 6.1.1.17

case <FTGroup:1>

Cofactor

Zn(2+)
Note: Binds 1 zinc ion per subunit.

end case

Subunit	Monomer.
Subcellular location	Cytoplasm.
Similarity	Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.

Keywords [?]

Aminoacyl-tRNA synthetase
ATP-binding
Cytoplasm
Ligase
Nucleotide-binding
Protein biosynthesis

case <FTGroup:1>

Metal-binding
Zinc

end case

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0004818; Molecular function: glutamate-tRNA ligase activity.

case <FTGroup:1>

GO:0008270; Molecular function: zinc ion binding.

end case

GO:0006424; Biological process: glutamyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam	PF00749; tRNA-synt_1c; 1;
PRINTS	PR00987; TRNASYNTHGLU; 1;
NCBIfam	TIGR00464; GltX_bact; 1;
PROSITE	PS00178; AA_TRNA_LIGASE_I; 1;

Features [?]

From: SYE_ECOLI (P04805)

Key From To Description Tag Condition FTGroup

MOTIF 9 19 'HIGH' region P-[SN]-P-x(4)-[HR]-[LIVMAT]-[GE]-x

MOTIF 237 241 'KMSKS' region x-[LIMSF]-S-K-[KR]

BINDING (Optional) 98 98 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 C 1

BINDING (Optional) 100 100 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 C 1

BINDING (Optional) 125 125 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 [CH] 1

BINDING (Optional) 127 127 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 [HED] 1

BINDING 240 240 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 K

Additional information [?]

Size range	431-546 amino acids
Related rules	MF_01428 (GLUQ)
Fusion	Nter: None; Cter: <Unknown>
Comments	This signature is specific for bacterial and eukaryotic mitochondrial glutamate--tRNA ligases. See MF_02076 for archaeal and eukaryotic cytoplasmic glutamate--tRNA ligases.

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