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HAMAP rule MF_00022

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General rule information [?]

Accession MF_00022
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 50)
Name Glu_tRNA_synth_type1
Scope(s) Bacteria
Template(s) P04805 (SYE_ECOLI); O25360 (SYE2_HELPY); P27000 (SYE_THET8); Q8DLI5 (SYE_THEVB); [ Recover all ]
Triggered by HAMAP; MF_00022 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier SYE
Protein name RecName: Full=Glutamate--tRNA ligase;
                 EC=6.1.1.17;
AltName: Full=Glutamyl-tRNA synthetase;
                 Short=GluRS;
Gene name Name=gltX;

Comments [?]

FUNCTIONCatalyzes the attachment of glutamate to tRNA(Glu) in a two- step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).
CATALYTIC ACTIVITY Reaction=ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L- glutamyl-tRNA(Glu); Xref=Rhea:RHEA:23540, Rhea:RHEA-COMP:9663, Rhea:RHEA-COMP:9680, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:78442, ChEBI:CHEBI:78520, ChEBI:CHEBI:456215; EC=6.1.1.17;
case <FTGroup:1>
COFACTOR Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit.;
end case
SUBUNITMonomer.
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYBelongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.

Keywords [?]


Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
GO:0004818; Molecular function:glutamate-tRNA ligase activity
case <FTGroup:1>
GO:0008270; Molecular function:zinc ion binding
end case
GO:0006424; Biological process:glutamyl-tRNA aminoacylation
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF00749; tRNA-synt_1c; 1;
PRINTS PR00987; TRNASYNTHGLU; 1;
NCBIfam TIGR00464; GltX_bact; 1;
PROSITE PS00178; AA_TRNA_LIGASE_I; 1;

Features [?]

From: SYE_ECOLI (P04805)
Key From To Description Tag Condition FTGroup
MOTIF 9 19 /note="'HIGH' region" P-[SN]-P-x(4)-[HR]-[LIVMAT]-[GE]-x
MOTIF 237 241 /note="'KMSKS' region" x-[LIMSF]-S-K-[KR]
BINDING 98 98 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
C 1
BINDING 100 100 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
C 1
BINDING 125 125 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
[CH] 1
BINDING 127 127 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
[HED] 1
BINDING 240 240 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
K

Additional information [?]

Size range 431-546 amino acids
Related rules MF_01428
Fusion Nter: None Cter: <Unknown>
Comments This signature is specific for bacterial and eukaryotic mitochondrial glutamate--tRNA ligases. See MF_02076 for archaeal and eukaryotic cytoplasmic glutamate--tRNA ligases.



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