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HAMAP rule MF_00023

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General rule information [?]

Accession MF_00023
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 26)
Name SmpB
Templates P0A832 (SSRP_ECOLI); Q8RR57 (SSRP_THET8); O66640 (SSRP_AQUAE); O32230 (SSRP_BACSU): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=SsrA-binding protein;
AltName: Full=Small protein B;
Gene name

Comments [?]

Function Required for rescue of stalled ribosomes mediated by trans-translation. Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to translate the ORF on the tmRNA; the nascent peptide is terminated with the 'tag peptide' encoded by the tmRNA and targeted for degradation. The ribosome is freed to recommence translation, which seems to be the essential function of trans-translation.
Subcellular location Cytoplasm. Note=The tmRNA-SmpB complex associates with stalled 70S ribosomes.
Similarity Belongs to the SmpB family.

Keywords [?]

Gene Ontology [?]

GO:0003723; Molecular function: RNA binding.
GO:0005737; Cellular component: cytoplasm.
GO:0070929; Biological process: trans-translation.

Cross-references [?]

Pfam PF01668; SmpB; 1;
NCBIfam TIGR00086; smpB; 1;

Additional information [?]

Size range 120-221 amino acids
Related rules None
Fusion None