HAMAP logo

HAMAP rule MF_00031

Send feedback

General rule information [?]

Accession MF_00031
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 29)
Name DNA_HJ_migration_RuvA
Scope(s) Bacteria
Template(s) P0A809 (RUVA_ECOLI); P40832 (RUVA_MYCLE); Q9F1Q3 (RUVA_THET8); P9WGW3 (RUVA_MYCTU); P66746 (RUVA_SALTY); [ Recover all ]
Triggered by HAMAP; MF_00031 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier RUVA
Protein name RecName: Full=Holliday junction branch migration complex subunit RuvA;
Gene name Name=ruvA;

Comments [?]

FUNCTIONThe RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair, while the RuvA-RuvB complex plays an important role in the rescue of blocked DNA replication forks via replication fork reversal (RFR). RuvA specifically binds to HJ cruciform DNA, conferring on it an open structure. The RuvB hexamer acts as an ATP-dependent pump, pulling dsDNA into and through the RuvAB complex. HJ branch migration allows RuvC to scan DNA until it finds its consensus sequence, where it cleaves and resolves the cruciform DNA.
SUBUNITHomotetramer. Forms an RuvA(8)-RuvB(12)-Holliday junction (HJ) complex. HJ DNA is sandwiched between 2 RuvA tetramers; dsDNA enters through RuvA and exits via RuvB. An RuvB hexamer assembles on each DNA strand where it exits the tetramer. Each RuvB hexamer is contacted by two RuvA subunits (via domain III) on 2 adjacent RuvB subunits; this complex drives branch migration. In the full resolvosome a probable DNA-RuvA(4)-RuvB(12)-RuvC(2) complex forms which resolves the HJ.
DOMAINHas three domains with a flexible linker between the domains II and III and assumes an 'L' shape. Domain III is highly mobile and contacts RuvB.
SIMILARITYBelongs to the RuvA family.

Keywords [?]

Gene Ontology [?]

GO:0005737; Cellular component:cytoplasm
GO:0000400; Molecular function:four-way junction DNA binding
GO:0048476; Cellular component:Holliday junction resolvase complex
GO:0006310; Biological process:DNA recombination
GO:0006281; Biological process:DNA repair
case <OCellular component:Escherichia>
GO:0009432; Biological process:SOS response
end case

Cross-references [?]

Pfam PF07499; RuvA_C; 1;
Pfam PF01330; RuvA_N; 1;
NCBIfam TIGR00084; ruvA; 1;

Features [?]

From: RUVA_ECOLI (P0A809)
Key From To Description Tag Condition FTGroup
REGION 1 64 /note="Domain I"
REGION 65 142 /note="Domain II"
REGION 143 155 /note="Flexible linker"
REGION 156 Cter /note="Domain III"

Additional information [?]

Size range 150-250 amino acids
Related rules None
Fusion Nter: None Cter: None

View rule in raw text format (no links)