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HAMAP rule MF_00034

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General rule information [?]

Accession MF_00034
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 35)
Name RuvC
Scope(s) Bacteria
Template(s) Q5SJC4 (RUVC_THET8); P0A814 (RUVC_ECOLI); Q51424 (RUVC_PSEAE); Q9RX75 (RUVC_DEIRA); [ Recover all ]
Triggered by HAMAP; MF_00034 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier RUVC
Protein name RecName: Full=Crossover junction endodeoxyribonuclease RuvC;
AltName: Full=Holliday junction nuclease RuvC;
AltName: Full=Holliday junction resolvase RuvC;
Gene name Name=ruvC;

Comments [?]

FUNCTIONThe RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair. Endonuclease that resolves HJ intermediates. Cleaves cruciform DNA by making single- stranded nicks across the HJ at symmetrical positions within the homologous arms, yielding a 5'-phosphate and a 3'-hydroxyl group; requires a central core of homology in the junction. The consensus cleavage sequence is 5'-(A/T)TT(C/G)-3'. Cleavage occurs on the 3'-side of the TT dinucleotide at the point of strand exchange. HJ branch migration catalyzed by RuvA-RuvB allows RuvC to scan DNA until it finds its consensus sequence, where it cleaves and resolves the cruciform DNA.
CATALYTIC ACTIVITY Reaction=Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).; EC=;
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 2 Mg(2+) ion per subunit.;
SUBUNITHomodimer which binds Holliday junction (HJ) DNA. The HJ becomes 2-fold symmetrical on binding to RuvC with unstacked arms; it has a different conformation from HJ DNA in complex with RuvA. In the full resolvosome a probable DNA-RuvA(4)-RuvB(12)-RuvC(2) complex forms which resolves the HJ.
SIMILARITYBelongs to the RuvC family.

Keywords [?]

Gene Ontology [?]

GO:0005737; Cellular component:cytoplasm
GO:0048476; Cellular component:Holliday junction resolvase complex
GO:0008821; Molecular function:crossover junction DNA endonuclease activity
GO:0000287; Molecular function:magnesium ion binding
GO:0006310; Biological process:DNA recombination
GO:0006281; Biological process:DNA repair

Cross-references [?]

Pfam PF02075; RuvC; 1;
NCBIfam TIGR00228; ruvC; 1;

Features [?]

Key From To Description Tag Condition FTGroup
ACT_SITE 70 70 E
ACT_SITE 143 143 [HD]
BINDING 7 7 /ligand="Mg(2+)"
BINDING 70 70 /ligand="Mg(2+)"
BINDING 143 143 /ligand="Mg(2+)"

Additional information [?]

Size range 100-300 amino acids
Related rules None
Fusion Nter: None Cter: None

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