HAMAP rule MF_00034
General rule information
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| Accession | MF_00034 |
| Dates | 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 35) |
| Name | RuvC |
| Scope(s) |
Bacteria |
| Template(s) | Q5SJC4 (RUVC_THET8); P0A814 (RUVC_ECOLI); Q51424 (RUVC_PSEAE); Q9RX75 (RUVC_DEIRA); [ Recover all ] |
| Triggered by |
HAMAP; MF_00034 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | RUVC |
| Protein name | RecName: Full=Crossover junction endodeoxyribonuclease RuvC; EC=3.1.21.10; AltName: Full=Holliday junction nuclease RuvC; AltName: Full=Holliday junction resolvase RuvC; |
| Gene name | Name=ruvC; |
Comments
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| FUNCTION | The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair. Endonuclease that resolves HJ intermediates. Cleaves cruciform DNA by making single- stranded nicks across the HJ at symmetrical positions within the homologous arms, yielding a 5'-phosphate and a 3'-hydroxyl group; requires a central core of homology in the junction. The consensus cleavage sequence is 5'-(A/T)TT(C/G)-3'. Cleavage occurs on the 3'-side of the TT dinucleotide at the point of strand exchange. HJ branch migration catalyzed by RuvA-RuvB allows RuvC to scan DNA until it finds its consensus sequence, where it cleaves and resolves the cruciform DNA. |
| CATALYTIC ACTIVITY | Reaction=Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).; EC=3.1.21.10; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 2 Mg(2+) ion per subunit.; |
| SUBUNIT | Homodimer which binds Holliday junction (HJ) DNA. The HJ becomes 2-fold symmetrical on binding to RuvC with unstacked arms; it has a different conformation from HJ DNA in complex with RuvA. In the full resolvosome a probable DNA-RuvA(4)-RuvB(12)-RuvC(2) complex forms which resolves the HJ. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the RuvC family. |
Keywords
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| Cytoplasm |
| Endonuclease |
| Hydrolase |
| Nuclease |
| DNA damage |
| DNA repair |
| DNA recombination |
| DNA-binding |
| Magnesium |
| Metal-binding |
Gene Ontology
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| GO:0005737; Cellular component:cytoplasm |
| GO:0048476; Cellular component:Holliday junction resolvase complex |
| GO:0008821; Molecular function:crossover junction DNA endonuclease activity |
| GO:0000287; Molecular function:magnesium ion binding |
| GO:0006310; Biological process:DNA recombination |
| GO:0006281; Biological process:DNA repair |
Cross-references
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Features
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| From: RUVC_THET8 (Q5SJC4) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 7 | 7 | D | |||||||||
| ACT_SITE | 70 | 70 | E | |||||||||
| ACT_SITE | 143 | 143 | [HD] | |||||||||
| BINDING | 7 | 7 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" |
D | ||||||||
| BINDING | 70 | 70 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" |
E | ||||||||
| BINDING | 143 | 143 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" |
[HD] | ||||||||
Additional information
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| Size range | 100-300 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |