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Annotation rule MF_00034
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General rule information [?]

Accession MF_00034
Dates 1-JUN-2001 (Created)
20-NOV-2019 (Last updated, Version 29)
Name RuvC
Scope
Bacteria
Template P0A814 (RUVC_ECOLI)
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
RUVC
Protein name
RecName: Full=Crossover junction endodeoxyribonuclease RuvC;
EC 3.1.22.4;
AltName: Full=Holliday junction nuclease RuvC;
AltName: Full=Holliday junction resolvase RuvC;
Gene name
ruvC

Comments [?]

Function Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.
Catalytic activity Reaction=Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).; EC=3.1.22.4;
case <FTGroup:1>
Cofactor Mg(2+)
Note: Binds 1 Mg(2+) ion per subunit.
end case
Similarity Belongs to the RuvC family.

Keywords [?]

Hydrolase
Nuclease
DNA damage
DNA repair
DNA recombination
case <FTGroup:1>
Magnesium
Metal-binding
end case

Gene Ontology [?]

GO:0008821; Molecular function: crossover junction endodeoxyribonuclease activity.
case <FTGroup:1>
GO:0000287; Molecular function: magnesium ion binding.
end case
GO:0006310; Biological process: DNA recombination.
GO:0006281; Biological process: DNA repair.

Cross-references [?]

Pfam PF02075; RuvC; 1;
TIGRFAMs TIGR00228; ruvC; 1;
PROSITE PS01321; RUVC; 1;

Features [?]

From: RUVC_ECOLI (P0A814)
Key     From     To       Description   Tag   Condition   FTGroup
METAL     8     8       Magnesium     D   1
METAL     67     67       Magnesium     E   1
METAL     139     139       Magnesium     [DH]   1
METAL     142     142       Magnesium     D   1

Additional information [?]

Size range 100-300 amino acids
Related rules None
Fusion None


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