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Annotation rule MF_00034 |
General rule information
[?]
Accession |
MF_00034 |
Dates |
1-JUN-2001 (Created) 20-NOV-2019 (Last updated, Version 29) |
Propagated annotation
[?]
Identifier, protein and gene names
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Protein name |
RecName:
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Full=Crossover junction endodeoxyribonuclease RuvC;
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EC 3.1.22.4;
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AltName:
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Full=Holliday junction nuclease RuvC;
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AltName:
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Full=Holliday junction resolvase RuvC;
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Function |
Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group. |
Catalytic activity |
Reaction=Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).; EC=3.1.22.4; |
case <FTGroup:1>
Cofactor |
Mg(2+) Note: Binds 1 Mg(2+) ion per subunit. |
end case
Similarity |
Belongs to the RuvC family. |
case <FTGroup:1>
end case
GO:0008821; Molecular function: crossover junction endodeoxyribonuclease activity.
case <FTGroup:1>
GO:0000287; Molecular function: magnesium ion binding.
end case
From: RUVC_ECOLI (P0A814) |
Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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METAL
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8
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8
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Magnesium
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D
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1
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METAL
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67
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67
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Magnesium
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E
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1
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METAL
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139
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139
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Magnesium
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[DH]
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1
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METAL
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142
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142
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Magnesium
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D
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1
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Additional information
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Size range |
100-300 amino acids |
Related rules |
None |
Fusion |
None |