HAMAP rule MF_00042
General rule information
[?]
| PURL | https://purl.expasy.org/hamap/rule/MF_00042 |
| Accession | MF_00042 |
| Dates | 1-JUN-2001 (Created)
19-NOV-2022 (Last updated, Version 36) |
| Name | RNase_H |
| Scope(s) |
Bacteria |
| Template(s) | P0A7Y4 (RNH_ECOLI); P29253 (RNH_THET8); [ Recover all ] |
| Triggered by |
HAMAP; MF_00042 (Get profile general information and statistics) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
| Identifier | RNH |
| Protein name | RecName: Full=Ribonuclease H; Short=RNase H; EC=3.1.26.4; |
| Gene name | Name=rnhA; |
Comments
[?]
| FUNCTION | Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. |
| CATALYTIC ACTIVITY | Reaction=Endonucleolytic cleavage to 5'-phosphomonoester.; EC=3.1.26.4; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding.; |
| SUBUNIT | Monomer. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the RNase H family. |
Keywords
[?]
Gene Ontology
[?]
| GO:0000287; Molecular function:magnesium ion binding |
| GO:0004523; Molecular function:RNA-DNA hybrid ribonuclease activity |
| GO:0006401; Biological process:RNA catabolic process |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
[?]
Features
[?]
| From: RNH_ECOLI (P0A7Y4) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 10 | 10 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" |
D | ||||||||
| BINDING | 10 | 10 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" |
D | ||||||||
| BINDING | 48 | 48 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" |
E | ||||||||
| BINDING | 70 | 70 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" |
D | ||||||||
| BINDING | 134 | 134 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" |
D | ||||||||
Additional information
[?]
| Size range | 143-231 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | Possible C-terminal domain in STRCO. Divergence in TREPA. |