HAMAP rule MF_00042

General rule information [?]

Accession	MF_00042
Dates	1-JUN-2001 (Created) 19-NOV-2022 (Last updated, Version 36)

Name

RNase_H

Scope

Bacteria

Templates

P0A7Y4 (RNH_ECOLI); P29253 (RNH_THET8): [Recover all]

Triggered by

HAMAP; MF_00042 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

RNH

Protein name

RecName:		Full=Ribonuclease H;
		Short=RNase H;
		EC 3.1.26.4;

Gene name

rnhA

Comments [?]

Function	Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.
Catalytic activity	Reaction=Endonucleolytic cleavage to 5'-phosphomonoester.; EC=3.1.26.4;
Cofactor	Mg(2+) Note: Binds 1 Mg(2+) ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding.
Subunit	Monomer.
Subcellular location	Cytoplasm.
Similarity	Belongs to the RNase H family.

Keywords [?]

Cytoplasm
Hydrolase
Nuclease
Endonuclease
Magnesium
Metal-binding

Gene Ontology [?]

GO:0000287; Molecular function: magnesium ion binding.
GO:0004523; Molecular function: RNA-DNA hybrid ribonuclease activity.
GO:0006401; Biological process: RNA catabolic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam	PF00075; RNase_H; 1;
PROSITE	PS50879; RNASE_H_1; 1; trigger=PRU00408;

Features [?]

From: RNH_ECOLI (P0A7Y4)

Key From To Description Tag Condition FTGroup

BINDING 10 10 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1 D

BINDING 10 10 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2 D

BINDING 48 48 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1 E

BINDING 70 70 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1 D

BINDING 134 134 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2 D

Additional information [?]

Size range	143-231 amino acids
Related rules	None
Fusion	None
Comments	Possible C-terminal domain in STRCO. Divergence in TREPA.

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