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HAMAP rule MF_00042

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General rule information [?]

Accession MF_00042
Dates 1-JUN-2001 (Created)
19-NOV-2022 (Last updated, Version 36)
Name RNase_H
Templates P0A7Y4 (RNH_ECOLI); P29253 (RNH_THET8): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Ribonuclease H;
Short=RNase H;
Gene name

Comments [?]

Function Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.
Catalytic activity Reaction=Endonucleolytic cleavage to 5'-phosphomonoester.; EC=;
Cofactor Mg(2+)
Note: Binds 1 Mg(2+) ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding.
Subunit Monomer.
Subcellular location Cytoplasm.
Similarity Belongs to the RNase H family.

Keywords [?]

Gene Ontology [?]

GO:0000287; Molecular function: magnesium ion binding.
GO:0004523; Molecular function: RNA-DNA hybrid ribonuclease activity.
GO:0006401; Biological process: RNA catabolic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00075; RNase_H; 1;
PROSITE PS50879; RNASE_H_1; 1; trigger=PRU00408;

Features [?]

From: RNH_ECOLI (P0A7Y4)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     10     10       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1     D  
BINDING     10     10       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2     D  
BINDING     48     48       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1     E  
BINDING     70     70       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1     D  
BINDING     134     134       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2     D  

Additional information [?]

Size range 143-231 amino acids
Related rules None
Fusion None
Comments Possible C-terminal domain in STRCO. Divergence in TREPA.