HAMAP rule MF_00061
General rule information
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| Accession | MF_00061 |
| Dates | 1-JUN-2001 (Created)
12-MAR-2024 (Last updated, Version 37) |
| Name | IspE |
| Scope(s) |
Bacteria |
| Template(s) | P62615 (ISPE_ECOLI); Q8FI04 (ISPE_ECOL6); P83700 (ISPE_THET8); [ Recover all ] |
| Triggered by |
HAMAP; MF_00061 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | ISPE |
| case <OC:Staphylococcus> or <OC:Streptococcus> | |
| Protein name | RecName: Full=Putative 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase; Short=CMK; EC=2.7.1.148; AltName: Full=4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase; |
| else | |
| Protein name | RecName: Full=4-diphosphocytidyl-2-C-methyl-D-erythritol kinase; Short=CMK; EC=2.7.1.148; AltName: Full=4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase; |
| Gene name | Name=ispE; |
| end case | |
Comments
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| FUNCTION | Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol. |
| CATALYTIC ACTIVITY | Reaction=4-CDP-2-C-methyl-D-erythritol + ATP = 4-CDP-2-C-methyl-D- erythritol 2-phosphate + ADP + H(+); Xref=Rhea:RHEA:18437, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:57823, ChEBI:CHEBI:57919, ChEBI:CHEBI:456216; EC=2.7.1.148; |
| case not <OC:Staphylococcus> and not <OC:Streptococcus> | |
| PATHWAY | Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 3/6. |
| end case | |
| case <OC:Enterobacterales> | |
| SUBUNIT | Homodimer. |
| end case | |
| SIMILARITY | Belongs to the GHMP kinase family. IspE subfamily. |
Keywords
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| ATP-binding | |
| Nucleotide-binding | |
| case not <OC:Staphylococcus> and not <OC:Streptococcus> | |
| Isoprene biosynthesis | |
| end case | |
| Kinase | |
| Transferase | |
Gene Ontology
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| GO:0050515; Molecular function:4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity | |
| GO:0005524; Molecular function:ATP binding | |
| case not <OCellular component:Staphylococcus> and not <OC:Streptococcus> | |
| GO:0019288; Biological process:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway | |
| end case | |
Cross-references
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| Pfam | PF08544; GHMP_kinases_C; 1; |
| Pfam | PF00288; GHMP_kinases_N; 1; |
| PIRSF | PIRSF010376; IspE; 1; |
| NCBIfam | TIGR00154; IspE; 1; |
Features
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| From: ISPE_ECOL6 (Q8FI04) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 99 | 109 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[PL]-x-x-[GSA]-G-[LIMFV]-[GA]-[GS]-[GAD]-S-[STAG] | ||||||||
| ACT_SITE | 10 | 10 | K | |||||||||
| ACT_SITE | 141 | 141 | D | |||||||||
Additional information
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| Size range | 255-329 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | In CHLPN there is a remnant of an ispE gene but which is interrupted by a probable natural frameshift (all CHLPN strains have it). It is orf CP0905 |