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Annotation rule MF_00061
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General rule information [?]

Accession MF_00061
Dates 1-JUN-2001 (Created)
20-NOV-2019 (Last updated, Version 33)
Name IspE
Scope
Bacteria
Templates P62615 (ISPE_ECOLI); Q8FI04 (ISPE_ECOL6); P83700 (ISPE_THET8): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
ISPE
case <OC:Staphylococcus> or <OC:Streptococcus>
Protein name
RecName: Full=Putative 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase;
Short=CMK;
EC 2.7.1.148;
AltName: Full=4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase;
else
Protein name
RecName: Full=4-diphosphocytidyl-2-C-methyl-D-erythritol kinase;
Short=CMK;
EC 2.7.1.148;
AltName: Full=4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase;
Gene name
ispE
end case

Comments [?]

Function Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol.
Catalytic activity RHEA:18437: 4-CDP-2-C-methyl-D-erythritol + ATP = 4-CDP-2-C-methyl-D-erythritol 2-phosphate + ADP + H(+)
EC 2.7.1.148
case not <OC:Staphylococcus> and not <OC:Streptococcus>
Pathway Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6.
end case
case <OC:Enterobacterales>
Subunit Homodimer.
end case
Similarity Belongs to the GHMP kinase family. IspE subfamily.

Keywords [?]

ATP-binding
Nucleotide-binding
case not <OC:Staphylococcus> and not <OC:Streptococcus>
Isoprene biosynthesis
end case
Kinase
Transferase

Gene Ontology [?]

GO:0050515; Molecular function: 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity.
GO:0005524; Molecular function: ATP binding.
case not <OC:Staphylococcus> and not <OC:Streptococcus>
GO:0016114; Biological process: terpenoid biosynthetic process.
end case

Cross-references [?]

Pfam PF08544; GHMP_kinases_C; 1;
PF00288; GHMP_kinases_N; 1;
PIRSF PIRSF010376; IspE; 1;
TIGRFAMs TIGR00154; IspE; 1;

Features [?]

From: ISPE_ECOL6 (Q8FI04)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     99     109       ATP     [PL]-x-x-[GSA]-G-[LIMFV]-[GA]-[GS]-[GAD]-S-[STAG]  
ACT_SITE     10     10             K  
ACT_SITE     141     141             D  

Additional information [?]

Size range 255-329 amino acids
Related rules None
Fusion None
Comments In CHLPN there is a remnant of an ispE gene but which is interrupted by a probable natural frameshift (all CHLPN strains have it). It is orf CP0905


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