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HAMAP rule MF_00061

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General rule information [?]

Accession MF_00061
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 36)
Name IspE
Scope(s) Bacteria
Template(s) P62615 (ISPE_ECOLI); Q8FI04 (ISPE_ECOL6); P83700 (ISPE_THET8); [ Recover all ]
Triggered by HAMAP; MF_00061 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier ISPE
case <OC:Staphylococcus> or <OC:Streptococcus>
Protein name RecName: Full=Putative 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase;
AltName: Full=4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase;
Protein name RecName: Full=4-diphosphocytidyl-2-C-methyl-D-erythritol kinase;
AltName: Full=4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase;
Gene name Name=ispE;
end case

Comments [?]

FUNCTIONCatalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol.
CATALYTIC ACTIVITY Reaction=4-CDP-2-C-methyl-D-erythritol + ATP = 4-CDP-2-C-methyl-D- erythritol 2-phosphate + ADP + H(+); Xref=Rhea:RHEA:18437, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:57823, ChEBI:CHEBI:57919, ChEBI:CHEBI:456216; EC=;
case not <OC:Staphylococcus> and not <OC:Streptococcus>
PATHWAYIsoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 3/6.
end case
case <OC:Enterobacterales>
end case
SIMILARITYBelongs to the GHMP kinase family. IspE subfamily.

Keywords [?]

case not <OC:Staphylococcus> and not <OC:Streptococcus>
Isoprene biosynthesis
end case

Gene Ontology [?]

GO:0050515; Molecular function:4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity
GO:0005524; Molecular function:ATP binding
case not <OCellular component:Staphylococcus> and not <OC:Streptococcus>
GO:0016114; Biological process:terpenoid biosynthetic process
end case

Cross-references [?]

Pfam PF08544; GHMP_kinases_C; 1;
Pfam PF00288; GHMP_kinases_N; 1;
PIRSF PIRSF010376; IspE; 1;
NCBIfam TIGR00154; IspE; 1;

Features [?]

From: ISPE_ECOL6 (Q8FI04)
Key From To Description Tag Condition FTGroup
BINDING 99 109 /ligand="ATP"
ACT_SITE 10 10 K
ACT_SITE 141 141 D

Additional information [?]

Size range 255-329 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments In CHLPN there is a remnant of an ispE gene but which is interrupted by a probable natural frameshift (all CHLPN strains have it). It is orf CP0905

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