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HAMAP rule MF_00062

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General rule information [?]

Accession MF_00062
Dates 1-JUN-2001 (Created)
14-MAY-2024 (Last updated, Version 32)
Name Sulf_adenylyltr_sub1
Scope(s) Bacteria
Template(s) P23845 (CYSN_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_00062 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier CYSN
Protein name RecName: Full=Sulfate adenylyltransferase subunit 1;
                 EC=2.7.7.4;
AltName: Full=ATP-sulfurylase large subunit;
AltName: Full=Sulfate adenylate transferase;
                 Short=SAT;
Gene name Name=cysN;

Comments [?]

FUNCTIONWith CysD forms the ATP sulfurylase (ATPS) that catalyzes the adenylation of sulfate producing adenosine 5'-phosphosulfate (APS) and diphosphate, the first enzymatic step in sulfur assimilation pathway. APS synthesis involves the formation of a high-energy phosphoric- sulfuric acid anhydride bond driven by GTP hydrolysis by CysN coupled to ATP hydrolysis by CysD.
CATALYTIC ACTIVITY Reaction=ATP + H(+) + sulfate = adenosine 5'-phosphosulfate + diphosphate; Xref=Rhea:RHEA:18133, ChEBI:CHEBI:15378, ChEBI:CHEBI:16189, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:58243; EC=2.7.7.4;
PATHWAYSulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3.
SUBUNITHeterodimer composed of CysD, the smaller subunit, and CysN.
SIMILARITYBelongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. CysN/NodQ subfamily.

Keywords [?]


Gene Ontology [?]

GO:0005525; Molecular function:GTP binding
GO:0004781; Molecular function:sulfate adenylyltransferase (ATP) activity
GO:0000103; Biological process:sulfate assimilation
GO:0070814; Biological process:hydrogen sulfide biosynthetic process

Cross-references [?]

Pfam PF00009; GTP_EFTU; 1;
PRINTS PR00315; ELONGATNFCT; 1;
PROSITE PS00301; G_TR_1; 1;
PROSITE PS51722; G_TR_2; 1;

Features [?]

From: CYSN_ECOLI (P23845)
Key From To Description Tag Condition FTGroup
BINDING 34 41 /ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"
G-x(4)-G-K-S
BINDING 113 117 /ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"
D-[ATC]-P-G-H
BINDING 168 171 /ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"
N-K-[IM]-D

Additional information [?]

Size range 441-494 amino acids
Related rules None
Fusion Nter: None Cter: MF_00065 (cysC)
Comments Problematic entry. N-terminal too short in MYCTU. If in a nodulating bacteria, has to be done manually because of the presence of both cysN and nodQ.



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