HAMAP rule MF_00062

General rule information [?]

Accession	MF_00062
Dates	1-JUN-2001 (Created) 14-MAY-2024 (Last updated, Version 32)
Name	Sulf_adenylyltr_sub1
Scope(s)	Bacteria
Template(s)	P23845 (CYSN_ECOLI); [ Recover all ]
Triggered by	HAMAP; MF_00062 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	CYSN
Protein name	RecName: Full=Sulfate adenylyltransferase subunit 1; EC=2.7.7.4; AltName: Full=ATP-sulfurylase large subunit; AltName: Full=Sulfate adenylate transferase; Short=SAT;
Gene name	Name=cysN;

Comments [?]

FUNCTION	With CysD forms the ATP sulfurylase (ATPS) that catalyzes the adenylation of sulfate producing adenosine 5'-phosphosulfate (APS) and diphosphate, the first enzymatic step in sulfur assimilation pathway. APS synthesis involves the formation of a high-energy phosphoric- sulfuric acid anhydride bond driven by GTP hydrolysis by CysN coupled to ATP hydrolysis by CysD.
CATALYTIC ACTIVITY	Reaction=ATP + H(+) + sulfate = adenosine 5'-phosphosulfate + diphosphate; Xref=Rhea:RHEA:18133, ChEBI:CHEBI:15378, ChEBI:CHEBI:16189, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:58243; EC=2.7.7.4;
PATHWAY	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3.
SUBUNIT	Heterodimer composed of CysD, the smaller subunit, and CysN.
SIMILARITY	Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. CysN/NodQ subfamily.

Keywords [?]

Gene Ontology [?]

GO:0005525; Molecular function:GTP binding

GO:0004781; Molecular function:sulfate adenylyltransferase (ATP) activity

GO:0000103; Biological process:sulfate assimilation

GO:0070814; Biological process:hydrogen sulfide biosynthetic process

Cross-references [?]

Features [?]

From: CYSN_ECOLI (P23845)

Key

From

Description

Tag

Condition

FTGroup

BINDING

/ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"

G-x(4)-G-K-S

BINDING

113

117

/ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"

D-[ATC]-P-G-H

BINDING

168

171

/ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"

N-K-[IM]-D

Additional information [?]

Size range	441-494 amino acids
Related rules	None
Fusion	Nter: None Cter: MF_00065 (cysC)
Comments	Problematic entry. N-terminal too short in MYCTU. If in a nodulating bacteria, has to be done manually because of the presence of both cysN and nodQ.