HAMAP rule MF_00062
General rule information
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| Accession | MF_00062 |
| Dates | 1-JUN-2001 (Created)
19-NOV-2022 (Last updated, Version 31) |
| Name | Sulf_adenylyltr_sub1 |
| Scope(s) |
Bacteria |
| Template(s) | P23845 (CYSN_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_00062 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | CYSN |
| Protein name | RecName: Full=Sulfate adenylyltransferase subunit 1; EC=2.7.7.4; AltName: Full=ATP-sulfurylase large subunit; AltName: Full=Sulfate adenylate transferase; Short=SAT; |
| Gene name | Name=cysN; |
Comments
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| FUNCTION | With CysD forms the ATP sulfurylase (ATPS) that catalyzes the adenylation of sulfate producing adenosine 5'-phosphosulfate (APS) and diphosphate, the first enzymatic step in sulfur assimilation pathway. APS synthesis involves the formation of a high-energy phosphoric- sulfuric acid anhydride bond driven by GTP hydrolysis by CysN coupled to ATP hydrolysis by CysD. |
| CATALYTIC ACTIVITY | Reaction=ATP + H(+) + sulfate = adenosine 5'-phosphosulfate + diphosphate; Xref=Rhea:RHEA:18133, ChEBI:CHEBI:15378, ChEBI:CHEBI:16189, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:58243; EC=2.7.7.4; |
| PATHWAY | Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3. |
| SUBUNIT | Heterodimer composed of CysD, the smaller subunit, and CysN. |
| SIMILARITY | Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. CysN/NodQ subfamily. |
Keywords
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Gene Ontology
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| GO:0005525; Molecular function:GTP binding |
| GO:0004781; Molecular function:sulfate adenylyltransferase (ATP) activity |
| GO:0000103; Biological process:sulfate assimilation |
Cross-references
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| Pfam | PF00009; GTP_EFTU; 1; |
| PRINTS | PR00315; ELONGATNFCT; 1; |
| PROSITE | PS00301; G_TR_1; 1; |
| PROSITE | PS51722; G_TR_2; 1; |
Features
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| From: CYSN_ECOLI (P23845) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 34 | 41 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
G-x(4)-G-K-S | ||||||||
| BINDING | 113 | 117 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
D-[ATC]-P-G-H | ||||||||
| BINDING | 168 | 171 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
N-K-[IM]-D | ||||||||
Additional information
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| Size range | 441-494 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: MF_00065 (cysC) |
| Comments | Problematic entry. N-terminal too short in MYCTU. If in a nodulating bacteria, has to be done manually because of the presence of both cysN and nodQ. |