HAMAP rule MF_00068
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_00068 |
| Accession | MF_00068 |
| Dates | 1-JUN-2001 (Created)
3-SEP-2024 (Last updated, Version 39) |
| Name | MurQ |
| Scope(s) |
Bacteria |
| Template(s) | P76535 (MURQ_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_00068 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | MURQ |
| Protein name | RecName: Full=N-acetylmuramic acid 6-phosphate etherase; Short=MurNAc-6-P etherase; EC=4.2.1.126; AltName: Full=N-acetylmuramic acid 6-phosphate hydrolase; AltName: Full=N-acetylmuramic acid 6-phosphate lyase; |
| Gene name | Name=murQ; |
Comments
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| case <OC:Pseudomonadota> | |
| FUNCTION | Specifically catalyzes the cleavage of the D-lactyl ether substituent of MurNAc 6-phosphate, producing GlcNAc 6-phosphate and D- lactate. Together with AnmK, is also required for the utilization of anhydro-N-acetylmuramic acid (anhMurNAc) either imported from the medium or derived from its own cell wall murein, and thus plays a role in cell wall recycling. |
| else | |
| FUNCTION | Specifically catalyzes the cleavage of the D-lactyl ether substituent of MurNAc 6-phosphate, producing GlcNAc 6-phosphate and D- lactate. |
| end case | |
| CATALYTIC ACTIVITY | Reaction=N-acetyl-D-muramate 6-phosphate + H2O = N-acetyl-D-glucosamine 6-phosphate + (R)-lactate; Xref=Rhea:RHEA:26410, ChEBI:CHEBI:15377, ChEBI:CHEBI:16004, ChEBI:CHEBI:57513, ChEBI:CHEBI:58722; EC=4.2.1.126; |
| PATHWAY | Amino-sugar metabolism; N-acetylmuramate degradation. |
| case <OC:Pseudomonadota> | |
| PATHWAY | Amino-sugar metabolism; 1,6-anhydro-N-acetylmuramate degradation. |
| PATHWAY | Cell wall biogenesis; peptidoglycan recycling. |
| end case | |
| SUBUNIT | Homodimer. |
| case <OC:Enterobacterales> | |
| INDUCTION | Induced by MurNAc 6-phosphate that releases the repressor MurR from the DNA. Repressed by MurR in the absence of MurNAc 6- phosphate. |
| end case | |
| MISCELLANEOUS | A lyase-type mechanism (elimination/hydration) is suggested for the cleavage of the lactyl ether bond of MurNAc 6- phosphate, with the formation of an alpha,beta-unsaturated aldehyde intermediate with (E)-stereochemistry, followed by the syn addition of water to give product. |
| SIMILARITY | Belongs to the GCKR-like family. MurNAc-6-P etherase subfamily. |
Keywords
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Gene Ontology
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| GO:0016835; Molecular function:carbon-oxygen lyase activity | |
| GO:0006040; Biological process:amino sugar metabolic process | |
| case <OCellular component:Pseudomonadota> | |
| GO:0097175; Biological process:1,6-anhydro-N-acetyl-beta-muramic acid catabolic process | |
| GO:0009254; Biological process:peptidoglycan turnover | |
| end case | |
Cross-references
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| PROSITE | PS01272; GCKR; 1; |
| PROSITE | PS51464; SIS; 1; |
| Pfam | PF01380; SIS; 1; |
| NCBIfam | TIGR00274; TIGR00274; 1; |
Features
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| From: MURQ_ECOLI (P76535) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 83 | 83 | /note="Proton donor" | E | ||||||||
| ACT_SITE | 114 | 114 | E | |||||||||
Additional information
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| Size range | 274-316 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |