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HAMAP rule MF_00071

General rule information [?]

Accession	MF_00071
Dates	1-JUN-2001 (Created) 1-JUN-2023 (Last updated, Version 30)
Name	LepA
Scope(s)	Bacteria
Template(s)	P60785 (LEPA_ECOLI); O67618 (LEPA_AQUAE); Q2FXY7 (LEPA_STAA8); [ Recover all ]
Triggered by	case c? <OC:Bacteria> HAMAP; MF_00071 (Get profile general information and statistics) end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	LEPA
RecName: Full=Elongation factor 4;<br />                  Short=EF-4;<br />                  EC=<a href="https://enzyme.expasy.org/EC/3.6.5.n1">3.6.5.n1</a>;<br /> AltName: Full=Ribosomal back-translocase LepA;
Gene name	Name=lepA;

Comments [?]

FUNCTION	Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre- translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP- dependent manner.
CATALYTIC ACTIVITY	Reaction=GTP + H2O = GDP + H(+) + phosphate; Xref=Rhea:RHEA:19669, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565, ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; EC=3.6.5.n1;
case not defined <Property:Membrane> or <Property:Membrane=1>
SUBCELLULAR LOCATION	Cell membrane; Peripheral membrane protein; Cytoplasmic side.
else case <Property:Membrane=2>
SUBCELLULAR LOCATION	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side.
end case
SIMILARITY	Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.

Keywords [?]

Cell membrane
case defined <Property:Membrane> and <Property:Membrane=2>
Cell inner membrane
end case
GTP-binding
Hydrolase
Membrane
Nucleotide-binding
Protein biosynthesis

Gene Ontology [?]

GO:0005886; Cellular component:plasma membrane

GO:0005525; Molecular function:GTP binding

GO:0003924; Molecular function:GTPase activity

GO:0043022; Molecular function:ribosome binding

GO:0003746; Molecular function:translation elongation factor activity

GO:0045727; Biological process:positive regulation of translation

Cross-references [?]

Features [?]

From: LEPA_AQUAE (O67618)

Key

From

Description

Tag

Condition

FTGroup

BINDING

/ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"

[DN]-H-G-K-[ST]-T

BINDING

133

136

/ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"

[NS]-K-[LIVMCA]-D

Additional information [?]