Home  |  Contact
Annotation rule MF_00071
Send feedback

General rule information [?]

Accession MF_00071
Dates 1-JUN-2001 (Created)
20-NOV-2019 (Last updated, Version 27)
Name LepA
Templates P60785 (LEPA_ECOLI); O67618 (LEPA_AQUAE); Q2FXY7 (LEPA_STAA8): [Recover all]
case <OC:Bacteria>
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Elongation factor 4;
EC 3.6.5.n1;
AltName: Full=Ribosomal back-translocase LepA;
Gene name

Comments [?]

Function Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.
Catalytic activity RHEA:19669: GTP + H2O = GDP + H(+) + phosphate
EC 3.6.5.n1
case not defined <Property:Membrane> or <Property:Membrane=1>
Subcellular location Cell membrane; Peripheral membrane protein; Cytoplasmic side.
else case <Property:Membrane=2>
Subcellular location Cell inner membrane; Peripheral membrane protein; Cytoplasmic side.
end case
Similarity Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.

Keywords [?]

case defined <Property:Membrane> and <Property:Membrane=2>
end case

Gene Ontology [?]

GO:0005886; Cellular component: plasma membrane.
GO:0005525; Molecular function: GTP binding.
GO:0003924; Molecular function: GTPase activity.
GO:0043022; Molecular function: ribosome binding.
GO:0003746; Molecular function: translation elongation factor activity.
GO:0045727; Biological process: positive regulation of translation.

Cross-references [?]

Pfam PF00679; EFG_C; 1;
PF00009; GTP_EFTU; 1;
PF03144; GTP_EFTU_D2; 1;
PF06421; LepA_C; 1;
TIGRFAMs TIGR01393; LepA; 1;
TIGR00231; Small_GTP; 1;
PROSITE PS00301; G_TR_1; 1;
PS51722; G_TR_2; 1;

Features [?]

From: LEPA_AQUAE (O67618)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     16     21       GTP     [DN]-H-G-K-[ST]-T  
NP_BIND     133     136       GTP     [NS]-K-[LIVMCA]-D  

Additional information [?]

Size range 593-660 amino acids
Related rules MF_03138 (GUFP supersedes the current rule)
Fusion None