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HAMAP rule MF_00071

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General rule information [?]

Accession MF_00071
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 30)
Name LepA
Scope(s) Bacteria
Template(s) P60785 (LEPA_ECOLI); O67618 (LEPA_AQUAE); Q2FXY7 (LEPA_STAA8); [ Recover all ]
Triggered by
case c? <OC:Bacteria>
HAMAP; MF_00071 (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier LEPA
RecName: Full=Elongation factor 4;<br /> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Short=EF-4;<br /> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;EC=<a href="https://enzyme.expasy.org/EC/3.6.5.n1">3.6.5.n1</a>;<br /> AltName: Full=Ribosomal back-translocase LepA;
Gene name Name=lepA;

Comments [?]

FUNCTIONRequired for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre- translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP- dependent manner.
CATALYTIC ACTIVITY Reaction=GTP + H2O = GDP + H(+) + phosphate; Xref=Rhea:RHEA:19669, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565, ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; EC=3.6.5.n1;
case not defined <Property:Membrane> or <Property:Membrane=1>
SUBCELLULAR LOCATIONCell membrane; Peripheral membrane protein; Cytoplasmic side.
else case <Property:Membrane=2>
SUBCELLULAR LOCATIONCell inner membrane; Peripheral membrane protein; Cytoplasmic side.
end case
SIMILARITYBelongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.

Keywords [?]

Cell membrane
case defined <Property:Membrane> and <Property:Membrane=2>
Cell inner membrane
end case
Protein biosynthesis

Gene Ontology [?]

GO:0005886; Cellular component:plasma membrane
GO:0005525; Molecular function:GTP binding
GO:0003924; Molecular function:GTPase activity
GO:0043022; Molecular function:ribosome binding
GO:0003746; Molecular function:translation elongation factor activity
GO:0045727; Biological process:positive regulation of translation

Cross-references [?]

Pfam PF00679; EFG_C; 1;
Pfam PF00009; GTP_EFTU; 1;
Pfam PF03144; GTP_EFTU_D2; 1;
Pfam PF06421; LepA_C; 1;
NCBIfam TIGR01393; LepA; 1;
NCBIfam TIGR00231; Small_GTP; 1;
PROSITE PS00301; G_TR_1; 1;
PROSITE PS51722; G_TR_2; 1;

Features [?]

From: LEPA_AQUAE (O67618)
Key From To Description Tag Condition FTGroup
BINDING 16 21 /ligand="GTP"
BINDING 133 136 /ligand="GTP"

Additional information [?]

Size range 593-660 amino acids
Related rules MF_03138
Fusion Nter: None Cter: None

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