Home  |  Contact
Annotation rule MF_00086
Send feedback

General rule information [?]

Accession MF_00086
Dates 1-JUN-2001 (Created)
20-NOV-2019 (Last updated, Version 44)
Name S_AdoMet_synth1
Scope
Bacteria
Template P0A817 (METK_ECOLI)

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
METK
Protein name
RecName: Full=S-adenosylmethionine synthase;
Short=AdoMet synthase;
EC 2.5.1.6;
AltName: Full=MAT;
AltName: Full=Methionine adenosyltransferase;
Gene name
metK

Comments [?]

Function Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.
Catalytic activity RHEA:21080: ATP + H2O + L-methionine = diphosphate + phosphate + S-adenosyl-L-methionine
EC 2.5.1.6
Cofactor Mg(2+)
Note: Binds 2 divalent ions per subunit.
K(+)
Note: Binds 1 potassium ion per subunit.
Pathway Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.
Subunit Homotetramer; dimer of dimers.
Subcellular location Cytoplasm.
Similarity Belongs to the AdoMet synthase family.

Keywords [?]


Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0004478; Molecular function: methionine adenosyltransferase activity.
GO:0006730; Biological process: one-carbon metabolic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF02773; S-AdoMet_synt_C; 1;
PF02772; S-AdoMet_synt_M; 1;
PF00438; S-AdoMet_synt_N; 1;
PIRSF PIRSF000497; MAT; 1;
TIGRFAMs TIGR01034; metK; 1;
PROSITE PS00376; ADOMET_SYNTHASE_1; 1;
PS00377; ADOMET_SYNTHASE_2; 1;

Features [?]

From: METK_ECOLI (P0A817)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND (Optional)     164     166       ATP     D-x-K  
NP_BIND (Optional)     230     231       ATP     [RK]-F  
NP_BIND (Optional)     245     246       ATP     R-K  
REGION     99     109       Flexible loop        
METAL     17     17       Magnesium     D  
METAL     43     43       Potassium     E  
BINDING (Optional)     15     15       ATP     H  
BINDING (Optional)     56     56       Methionine     E  
BINDING (Optional)     99     99       Methionine     Q  
BINDING (Optional)     239     239       ATP; shared with neighboring subunit     D  
BINDING (Optional)     239     239       Methionine; shared with neighboring subunit     D  
BINDING (Optional)     262     262       ATP; via amide nitrogen; shared with neighboring subunit     [AS]  
BINDING (Optional)     266     266       ATP; shared with neighboring subunit     K  
BINDING (Optional)     270     270       Methionine     K  

Additional information [?]

Size range 376-430 amino acids
Related rules None
Fusion None