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| Annotation rule MF_00086 |
General rule information
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| Accession | MF_00086 |
| Dates | 1-JUN-2001 (Created) 20-NOV-2019 (Last updated, Version 44) |
| Name | S_AdoMet_synth1 |
| Scope | Bacteria |
| Template | P0A817 (METK_ECOLI) |
| Triggered by |
Propagated annotation
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Identifier, protein and gene names
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| Identifier |
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| Protein name |
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| Gene name |
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Comments
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| Function | Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme. |
| Catalytic activity | RHEA:21080: ATP + H2O + L-methionine = diphosphate + phosphate + S-adenosyl-L-methionine
EC 2.5.1.6 |
| Cofactor | Mg(2+) Note: Binds 2 divalent ions per subunit. |
| K(+) Note: Binds 1 potassium ion per subunit. |
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| Pathway | Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1. |
| Subunit | Homotetramer; dimer of dimers. |
| Subcellular location | Cytoplasm. |
| Similarity | Belongs to the AdoMet synthase family. |
Keywords
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ATP-binding
Cytoplasm
Magnesium
Metal-binding
Nucleotide-binding
One-carbon metabolism
Potassium
Transferase
Cytoplasm
Magnesium
Metal-binding
Nucleotide-binding
One-carbon metabolism
Potassium
Transferase
Gene Ontology
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GO:0005524; Molecular function: ATP binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0004478; Molecular function: methionine adenosyltransferase activity.
GO:0006730; Biological process: one-carbon metabolic process.
GO:0005737; Cellular component: cytoplasm.
GO:0000287; Molecular function: magnesium ion binding.
GO:0004478; Molecular function: methionine adenosyltransferase activity.
GO:0006730; Biological process: one-carbon metabolic process.
GO:0005737; Cellular component: cytoplasm.
Cross-references
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| Pfam | PF02773; S-AdoMet_synt_C; 1; |
| PF02772; S-AdoMet_synt_M; 1; | |
| PF00438; S-AdoMet_synt_N; 1; | |
| PIRSF | PIRSF000497; MAT; 1; |
| TIGRFAMs | TIGR01034; metK; 1; |
| PROSITE | PS00376; ADOMET_SYNTHASE_1; 1; |
| PS00377; ADOMET_SYNTHASE_2; 1; |
Features
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| From: METK_ECOLI (P0A817) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| NP_BIND (Optional) | 164 | 166 | ATP | D-x-K | ||||||||
| NP_BIND (Optional) | 230 | 231 | ATP | [RK]-F | ||||||||
| NP_BIND (Optional) | 245 | 246 | ATP | R-K | ||||||||
| REGION | 99 | 109 | Flexible loop | |||||||||
| METAL | 17 | 17 | Magnesium | D | ||||||||
| METAL | 43 | 43 | Potassium | E | ||||||||
| BINDING (Optional) | 15 | 15 | ATP | H | ||||||||
| BINDING (Optional) | 56 | 56 | Methionine | E | ||||||||
| BINDING (Optional) | 99 | 99 | Methionine | Q | ||||||||
| BINDING (Optional) | 239 | 239 | ATP; shared with neighboring subunit | D | ||||||||
| BINDING (Optional) | 239 | 239 | Methionine; shared with neighboring subunit | D | ||||||||
| BINDING (Optional) | 262 | 262 | ATP; via amide nitrogen; shared with neighboring subunit | [AS] | ||||||||
| BINDING (Optional) | 266 | 266 | ATP; shared with neighboring subunit | K | ||||||||
| BINDING (Optional) | 270 | 270 | Methionine | K | ||||||||
Additional information
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| Size range | 376-430 amino acids |
| Related rules | None |
| Fusion | None |