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HAMAP rule MF_00086
General rule information
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| Accession | MF_00086 |
| Dates | 1-JUN-2001 (Created)
15-FEB-2024 (Last updated, Version 50) |
| Name | S_AdoMet_synth1 |
| Scope(s) |
Bacteria |
| Template(s) | P0A817 (METK_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_00086 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | METK |
| Protein name | RecName: Full=S-adenosylmethionine synthase; Short=AdoMet synthase; EC=2.5.1.6; AltName: Full=MAT; AltName: Full=Methionine adenosyltransferase; |
| Gene name | Name=metK; |
Comments
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| FUNCTION | Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme. |
| CATALYTIC ACTIVITY | Reaction=ATP + H2O + L-methionine = diphosphate + phosphate + S- adenosyl-L-methionine; Xref=Rhea:RHEA:21080, ChEBI:CHEBI:15377, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:43474, ChEBI:CHEBI:57844, ChEBI:CHEBI:59789; EC=2.5.1.6; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 2 divalent ions per subunit.; |
| COFACTOR | Name=K(+); Xref=ChEBI:CHEBI:29103; Note=Binds 1 potassium ion per subunit.; |
| PATHWAY | Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1. |
| SUBUNIT | Homotetramer; dimer of dimers. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the AdoMet synthase family. |
Keywords
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| ATP-binding |
| Cytoplasm |
| Magnesium |
| Metal-binding |
| Nucleotide-binding |
| One-carbon metabolism |
| Potassium |
| Transferase |
Gene Ontology
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| GO:0005524; Molecular function:ATP binding |
| GO:0000287; Molecular function:magnesium ion binding |
| GO:0004478; Molecular function:methionine adenosyltransferase activity |
| GO:0006556; Biological process:S-adenosylmethionine biosynthetic process |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| Pfam | PF02773; S-AdoMet_synt_C; 1; |
| Pfam | PF02772; S-AdoMet_synt_M; 1; |
| Pfam | PF00438; S-AdoMet_synt_N; 1; |
| PIRSF | PIRSF000497; MAT; 1; |
| NCBIfam | TIGR01034; metK; 1; |
| PROSITE | PS00376; ADOMET_SYNTHASE_1; 1; |
| PROSITE | PS00377; ADOMET_SYNTHASE_2; 1; |
Features
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| From: METK_ECOLI (P0A817) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 164 | 166 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" /ligand_note="ligand shared between two neighboring subunits" /note="in other chain" |
D-x-K | ||||||||
| BINDING | 230 | 231 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" /ligand_note="ligand shared between two neighboring subunits" /note="in other chain" |
[RK]-F | ||||||||
| BINDING | 245 | 246 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" /ligand_note="ligand shared between two neighboring subunits" /note="in other chain" |
R-K | ||||||||
| REGION | 99 | 109 | /note="Flexible loop" | |||||||||
| BINDING | 17 | 17 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | ||||||||
| BINDING | 43 | 43 | /ligand="K(+)" /ligand_id="ChEBI:CHEBI:29103" |
E | ||||||||
| BINDING | 15 | 15 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" /ligand_note="ligand shared between two neighboring subunits" /note="in other chain" |
H | ||||||||
| BINDING | 56 | 56 | /ligand="L-methionine" /ligand_id="ChEBI:CHEBI:57844" /ligand_note="ligand shared between two neighboring subunits" /note="in other chain" |
E | ||||||||
| BINDING | 99 | 99 | /ligand="L-methionine" /ligand_id="ChEBI:CHEBI:57844" /ligand_note="ligand shared between two neighboring subunits" /note="in other chain" |
Q | ||||||||
| BINDING | 239 | 239 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" /ligand_note="ligand shared between two neighboring subunits" |
D | ||||||||
| BINDING | 239 | 239 | /ligand="L-methionine" /ligand_id="ChEBI:CHEBI:57844" /ligand_note="ligand shared between two neighboring subunits" |
D | ||||||||
| BINDING | 262 | 262 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" /ligand_note="ligand shared between two neighboring subunits" |
[AS] | ||||||||
| BINDING | 266 | 266 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" /ligand_note="ligand shared between two neighboring subunits" |
K | ||||||||
| BINDING | 270 | 270 | /ligand="L-methionine" /ligand_id="ChEBI:CHEBI:57844" /ligand_note="ligand shared between two neighboring subunits" /note="in other chain" |
K | ||||||||
Additional information
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| Size range | 376-430 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |