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Annotation rule MF_00086 |
Accession | MF_00086 |
Dates | 1-JUN-2001 (Created) 20-NOV-2019 (Last updated, Version 44) |
Name | S_AdoMet_synth1 |
Scope | Bacteria |
Template | P0A817 (METK_ECOLI) |
Triggered by |
Identifier |
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Protein name |
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Gene name |
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Function | Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme. |
Catalytic activity | RHEA:21080: ATP + H2O + L-methionine = diphosphate + phosphate + S-adenosyl-L-methionine
EC 2.5.1.6 |
Cofactor | Mg(2+) Note: Binds 2 divalent ions per subunit. |
K(+) Note: Binds 1 potassium ion per subunit. |
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Pathway | Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1. |
Subunit | Homotetramer; dimer of dimers. |
Subcellular location | Cytoplasm. |
Similarity | Belongs to the AdoMet synthase family. |
Pfam | PF02773; S-AdoMet_synt_C; 1; |
PF02772; S-AdoMet_synt_M; 1; | |
PF00438; S-AdoMet_synt_N; 1; | |
PIRSF | PIRSF000497; MAT; 1; |
TIGRFAMs | TIGR01034; metK; 1; |
PROSITE | PS00376; ADOMET_SYNTHASE_1; 1; |
PS00377; ADOMET_SYNTHASE_2; 1; |
From: METK_ECOLI (P0A817) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
NP_BIND (Optional) | 164 | 166 | ATP | D-x-K | ||||||||
NP_BIND (Optional) | 230 | 231 | ATP | [RK]-F | ||||||||
NP_BIND (Optional) | 245 | 246 | ATP | R-K | ||||||||
REGION | 99 | 109 | Flexible loop | |||||||||
METAL | 17 | 17 | Magnesium | D | ||||||||
METAL | 43 | 43 | Potassium | E | ||||||||
BINDING (Optional) | 15 | 15 | ATP | H | ||||||||
BINDING (Optional) | 56 | 56 | Methionine | E | ||||||||
BINDING (Optional) | 99 | 99 | Methionine | Q | ||||||||
BINDING (Optional) | 239 | 239 | ATP; shared with neighboring subunit | D | ||||||||
BINDING (Optional) | 239 | 239 | Methionine; shared with neighboring subunit | D | ||||||||
BINDING (Optional) | 262 | 262 | ATP; via amide nitrogen; shared with neighboring subunit | [AS] | ||||||||
BINDING (Optional) | 266 | 266 | ATP; shared with neighboring subunit | K | ||||||||
BINDING (Optional) | 270 | 270 | Methionine | K |
Size range | 376-430 amino acids |
Related rules | None |
Fusion | None |