HAMAP rule MF_00092

General rule information [?]

PURL	https://purl.expasy.org/hamap/rule/MF_00092
Accession	MF_00092
Dates	28-FEB-2005 (Created) 02-SEP-2024 (Last updated, Version 21)
Name	MutS2
Scope(s)	Bacteria
Template(s)	Q5SHT5; O25338; Q9X105; [ Recover all ]
Triggered by	HAMAP; MF_00092 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	MUTS2
Protein name	RecName: Full=Endonuclease MutS2; EC=3.1.-.-; AltName: Full=Ribosome-associated protein quality control-upstream factor; Short=RQC-upstream factor; Short=RqcU; EC=3.6.4.-;
Gene name	Name=mutS2; Synonyms=rqcU;

Comments [?]

FUNCTION	Endonuclease that is involved in the suppression of homologous recombination and thus may have a key role in the control of bacterial genetic diversity.
FUNCTION	Acts as a ribosome collision sensor, splitting the ribosome into its 2 subunits. Detects stalled/collided 70S ribosomes which it binds and splits by an ATP-hydrolysis driven conformational change. Acts upstream of the ribosome quality control system (RQC), a ribosome- associated complex that mediates the extraction of incompletely synthesized nascent chains from stalled ribosomes and their subsequent degradation. Probably generates substrates for RQC.
SUBUNIT	Homodimer. Binds to stalled ribosomes, contacting rRNA.
SIMILARITY	Belongs to the DNA mismatch repair MutS family. MutS2 subfamily.

Keywords [?]

Gene Ontology [?]

GO:0005524; Molecular function:ATP binding

GO:0003677; Molecular function:DNA binding

GO:0004519; Molecular function:endonuclease activity

GO:0072344; Biological process:rescue of stalled ribosome

GO:0019843; Molecular function:rRNA binding

GO:0043023; Molecular function:ribosomal large subunit binding

Cross-references [?]

Features [?]

From: MUTS2_THET8 (Q5SHT5)

Key

From

Description

Tag

Condition

FTGroup

BINDING

315

322

/ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"

G-x-N-x-G-G-K-[TS]

Additional information [?]

Size range	660-920 amino acids
Related rules	None
Fusion	Nter: None Cter: None
Comments	Endonuclease activity has been proven for T.thermophilus (Q5SHT5), T. maritima (Q9X105), D.radiodurans (Q9RSZ3) but could not be demonstrated in B. subtilis (P94545).