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Annotation rule MF_00094
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General rule information [?]

Accession MF_00094
Dates 1-JUN-2001 (Created)
20-NOV-2019 (Last updated, Version 20)
Name Rel_fac_2
Scope
Bacteria
Template P07012 (RF2_ECOLI)
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
RF2
Protein name
RecName: Full=Peptide chain release factor 2;
Short=RF-2;
Gene name
prfB

Comments [?]

Function Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA.
Subcellular location Cytoplasm.
case <FT:1>
Ptm Methylated by PrmC. Methylation increases the termination efficiency of RF2.
end case
Similarity Belongs to the prokaryotic/mitochondrial release factor family.

Keywords [?]

Cytoplasm
Protein biosynthesis
case <FT:1>
Methylation
end case

Gene Ontology [?]

GO:0016149; Molecular function: translation release factor activity, codon specific.
GO:0006415; Biological process: translational termination.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00472; RF-1; 1;
TIGRFAMs TIGR00020; PrfB; 1;
PROSITE PS00745; RF_PROK_I; 1;

Features [?]

From: RF2_ECOLI (P07012)
Key     From     To       Description   Tag   Condition   FTGroup
MOD_RES     252     252       N5-methylglutamine     Q  

Additional information [?]

Size range 359-377 amino acids
Related rules MF_00093 (RF1)
Fusion None
Comments In about 70% of bacterial genomes the mRNA encoding RF-2 has 2 partially overlapping reading frames. Synthesis of full-length RF-2 requires +1 frameshifting at the end of the first open reading frame, usually on a UGA codon. This frameshifting serves an autoregulatory function; when RF-2 levels are low frameshifting occurs and full-length protein is produced.


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