HAMAP rule MF_00104
General rule information
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| Accession | MF_00104 |
| Dates | 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 34) |
| Name | RNase_III |
| Scope(s) |
Bacteria |
| Template(s) | O67082 (RNC_AQUAE); P51833 (RNC_BACSU); P0A7Y0 (RNC_ECOLI); Q52698 (RNC_RHOCA); Q56056 (RNC_SALTY); Q9ZBQ7 (RNC_STRCO); Q9X0I6 (RNC_THEMA); [ Recover all ] |
| Triggered by |
HAMAP; MF_00104 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | RNC |
| Protein name | RecName: Full=Ribonuclease 3; EC=3.1.26.3; AltName: Full=Ribonuclease III; Short=RNase III; |
| Gene name | Name=rnc; |
Comments
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| FUNCTION | Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism. |
| CATALYTIC ACTIVITY | Reaction=Endonucleolytic cleavage to 5'-phosphomonoester.; EC=3.1.26.3; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| SUBUNIT | Homodimer. |
| SUBCELLULAR LOCATION | Cytoplasm. |
Keywords
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| Cytoplasm | |
| Hydrolase | |
| Nuclease | |
| Endonuclease | |
| case <FT:3> or <FT:4> or <FT:5> | |
| Magnesium | |
| Metal-binding | |
| end case | |
| case <Feature:PS50137> | |
| RNA-binding | |
| rRNA-binding | |
| end case | |
| mRNA processing | |
| rRNA processing | |
| tRNA processing | |
Gene Ontology
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| GO:0004525; Molecular function:ribonuclease III activity |
| GO:0006397; Biological process:mRNA processing |
| GO:0006364; Biological process:rRNA processing |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| Pfam | PF00035; dsrm; 1; |
| Pfam | PF00636; Ribonuclease_3; 1; |
| NCBIfam | TIGR02191; RNaseIII; 1; |
| PROSITE | PS50137; DS_RBD; 1; |
| PROSITE | PS00517; RNASE_3_1; 1; |
| PROSITE | PS50142; RNASE_3_2; 1; |
Features
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| From: RNC_AQUAE (O67082) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 44 | 44 | D | |||||||||
| ACT_SITE | 110 | 110 | E | |||||||||
| BINDING | 40 | 40 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
E | ||||||||
| BINDING | 107 | 107 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
[DENS] | ||||||||
| BINDING | 110 | 110 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
E | ||||||||
Additional information
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| Size range | 221-369 amino acids |
| Related rules |
None |
| Fusion | Nter: <Unknown> Cter: None |
| Comments | Often predicted too short. Some Mycoplasmas lack the DRBM domain. Mycoplasma gallisepticum strains have an unknown N-terminal extension of about 400 aa. ROSHA and TRES6 are slightly longer in the middle; ROSHA has 2 DRBM domains. |