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HAMAP rule MF_00112

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General rule information [?]

Accession MF_00112
Dates 1-JUN-2001 (Created)
2-SEP-2024 (Last updated, Version 34)
Name GGGP_HepGP_synthase
Scope(s) Bacteria
Bacillales
Bacteroidota
Archaea
Template(s) O29844 (GGGPS_ARCFU); B0R2Z3 (GGGPS_HALS3); Q9YEF5 (GGGPS_AERPE); O26652 (GGGPS_METTH); P0C882 (GGGPS_METTM); A6VHZ5 (GGGPS_METM7); Q980N1 (GGGPS_SACS2); Q5JDY1 (GGGPS_THEKO); Q9HJH3 (GGGPS_THEAC); O34790 (PCRB_BACSU); Q5L3C1 (PCRB_GEOKA); Q8Y6C8 (PCRB_LISMO); Q53726 (PCRB_STAA8); A5FJK8 (GGGPS_FLAJ1); [ Recover all ]
Triggered by HAMAP; MF_00112 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

case <OC:Archaea> or <OC:Bacteroidota>
Identifier GGGPS
Protein name RecName: Full=Geranylgeranylglyceryl phosphate synthase;
                 Short=GGGP synthase;
                 Short=GGGPS;
                 EC=2.5.1.41;
AltName: Full=(S)-3-O-geranylgeranylglyceryl phosphate synthase;
AltName: Full=Phosphoglycerol geranylgeranyltransferase;
else case <OC:Bacillales>
Identifier PCRB
Protein name RecName: Full=Heptaprenylglyceryl phosphate synthase;
                 Short=HepGP synthase;
                 EC=2.5.1.n9;
AltName: Full=Glycerol-1-phosphate heptaprenyltransferase;
Gene name Name=pcrB;
end case

Comments [?]

case <OC:Archaea>
FUNCTIONPrenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P). This reaction is the first ether-bond-formation step in the biosynthesis of archaeal membrane lipids.
CATALYTIC ACTIVITY Reaction=sn-glycerol 1-phosphate + (2E,6E,10E)-geranylgeranyl diphosphate = sn-3-O-(geranylgeranyl)glycerol 1-phosphate + diphosphate; Xref=Rhea:RHEA:23404, ChEBI:CHEBI:33019, ChEBI:CHEBI:57677, ChEBI:CHEBI:57685, ChEBI:CHEBI:58756; EC=2.5.1.41;
PATHWAYMembrane lipid metabolism; glycerophospholipid metabolism.
SUBCELLULAR LOCATIONCytoplasm.
else case <OC:Bacteroidota>
FUNCTIONPrenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P).
CATALYTIC ACTIVITY Reaction=sn-glycerol 1-phosphate + (2E,6E,10E)-geranylgeranyl diphosphate = sn-3-O-(geranylgeranyl)glycerol 1-phosphate + diphosphate; Xref=Rhea:RHEA:23404, ChEBI:CHEBI:33019, ChEBI:CHEBI:57677, ChEBI:CHEBI:57685, ChEBI:CHEBI:58756; EC=2.5.1.41;
else case <OC:Bacillales>
FUNCTIONPrenyltransferase that catalyzes in vivo the transfer of the heptaprenyl moiety of heptaprenyl pyrophosphate (HepPP; 35 carbon atoms) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P), producing heptaprenylglyceryl phosphate (HepGP). This reaction is an ether-bond- formation step in the biosynthesis of archaea-type G1P-based membrane lipids found in Bacillales.
CATALYTIC ACTIVITY Reaction=sn-glycerol 1-phosphate + all-trans-heptaprenyl diphosphate = 3-heptaprenyl-sn-glycero-1-phosphate + diphosphate; Xref=Rhea:RHEA:33495, ChEBI:CHEBI:33019, ChEBI:CHEBI:57685, ChEBI:CHEBI:58206, ChEBI:CHEBI:64781; EC=2.5.1.n9;
PATHWAYMembrane lipid metabolism; glycerophospholipid metabolism.
SUBUNITHomodimer.
end case
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
case <FTGroup:1>
SIMILARITYBelongs to the GGGP/HepGP synthase family. Group I subfamily.
else case <FTGroup:2>
SIMILARITYBelongs to the GGGP/HepGP synthase family. Group II subfamily.
else
SIMILARITYBelongs to the GGGP/HepGP synthase family.
end case

Keywords [?]

case <OC:Archaea>
Cytoplasm
end case
Lipid biosynthesis
Lipid metabolism
Magnesium
Metal-binding
Phospholipid biosynthesis
Phospholipid metabolism
Transferase

Gene Ontology [?]

case <OCellular component:Archaea> or <OC:Bacteroidota>
GO:0047294; Molecular function:phosphoglycerol geranylgeranyltransferase activity
else case <OCellular component:Bacillales>
GO:0002094; Molecular function:polyprenyltransferase activity
end case
GO:0000287; Molecular function:magnesium ion binding
GO:0046474; Biological process:glycerophospholipid biosynthetic process
case <OCellular component:Archaea>
GO:0005737; Cellular component:cytoplasm
end case

Cross-references [?]

Pfam PF01884; PcrB; 1;
NCBIfam TIGR01768; GGGP-family; 1;
NCBIfam TIGR01769; GGGP; 0-1;
NCBIfam TIGR04146; GGGPS_Afulg; 0-1;
NCBIfam TIGR04147; GGGPS_Halobact; 0-1;

Features [?]

From: GGGPS_ARCFU (O29844)
Key From To Description Tag Condition FTGroup
BINDING 165 170 /ligand="sn-glycerol 1-phosphate"
/ligand_id="ChEBI:CHEBI:57685"		 Y-[IVLM]-E-Y-S-G 1
BINDING 215 216 /ligand="sn-glycerol 1-phosphate"
/ligand_id="ChEBI:CHEBI:57685"		 G-[ND] 1
BINDING 13 13 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"		 D
BINDING 39 39 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"		 [ST]
BINDING 11 11 /ligand="sn-glycerol 1-phosphate"
/ligand_id="ChEBI:CHEBI:57685"		 K 1
BINDING 195 195 /ligand="sn-glycerol 1-phosphate"
/ligand_id="ChEBI:CHEBI:57685"		 G 1
From: GGGPS_METTH (O26652)
Key From To Description Tag Condition FTGroup
BINDING 169 175 /ligand="sn-glycerol 1-phosphate"
/ligand_id="ChEBI:CHEBI:57685"		 [YF]-[LMFI]-[ED]-[AGS]-G-S-G 2
BINDING 200 201 /ligand="sn-glycerol 1-phosphate"
/ligand_id="ChEBI:CHEBI:57685"		 G-G 2
BINDING 222 223 /ligand="sn-glycerol 1-phosphate"
/ligand_id="ChEBI:CHEBI:57685"		 G-[TNS] 2

Additional information [?]

Size range 210-304 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments Bacteroidota have GGGPS activity like the archaeal enzyme, but the physiological function is unknown (PubMed=24684232). Two phylogenetic subgroups (I and II) exist within the family. They use alternative amino acid residues to bind G1P (PubMed=24684232). Many Halobacteria possess two paralogs of GGGPS.



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