HAMAP rule MF_00112
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_00112 |
| Accession | MF_00112 |
| Dates | 28-FEB-2005 (Created)
21-MAR-2025 (Last updated, Version 30) |
| Name | GGGP_HepGP_synthase |
| Scope(s) |
Bacteria Bacillales Bacteroidota Archaea |
| Template(s) | O29844; B0R2Z3; Q9YEF5; O26652; P0C882; A6VHZ5; Q980N1; Q5JDY1; Q9HJH3; O34790; Q5L3C1; Q8Y6C8; Q53726; A5FJK8; [ Recover all ] |
| Triggered by |
HAMAP; MF_00112 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| case <OC:Archaea> or <OC:Bacteroidota> | |
| Identifier | GGGPS |
| Protein name | RecName: Full=Geranylgeranylglyceryl phosphate synthase; Short=GGGP synthase; Short=GGGPS; EC=2.5.1.41; AltName: Full=(S)-3-O-geranylgeranylglyceryl phosphate synthase; AltName: Full=Phosphoglycerol geranylgeranyltransferase; |
| else case <OC:Bacillales> | |
| Identifier | PCRB |
| Protein name | RecName: Full=Heptaprenylglyceryl phosphate synthase; Short=HepGP synthase; EC=2.5.1.n9; AltName: Full=Glycerol-1-phosphate heptaprenyltransferase; |
| Gene name | Name=pcrB; |
| end case | |
Comments
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| case <OC:Archaea> | |
| FUNCTION | Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P). This reaction is the first ether-bond-formation step in the biosynthesis of archaeal membrane lipids. |
| CATALYTIC ACTIVITY | Reaction=sn-glycerol 1-phosphate + (2E,6E,10E)-geranylgeranyl diphosphate = sn-3-O-(geranylgeranyl)glycerol 1-phosphate + diphosphate; Xref=Rhea:RHEA:23404, ChEBI:CHEBI:33019, ChEBI:CHEBI:57677, ChEBI:CHEBI:57685, ChEBI:CHEBI:58756; EC=2.5.1.41; |
| PATHWAY | Membrane lipid metabolism; glycerophospholipid metabolism. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| else case <OC:Bacteroidota> | |
| FUNCTION | Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P). |
| CATALYTIC ACTIVITY | Reaction=sn-glycerol 1-phosphate + (2E,6E,10E)-geranylgeranyl diphosphate = sn-3-O-(geranylgeranyl)glycerol 1-phosphate + diphosphate; Xref=Rhea:RHEA:23404, ChEBI:CHEBI:33019, ChEBI:CHEBI:57677, ChEBI:CHEBI:57685, ChEBI:CHEBI:58756; EC=2.5.1.41; |
| else case <OC:Bacillales> | |
| FUNCTION | Prenyltransferase that catalyzes in vivo the transfer of the heptaprenyl moiety of heptaprenyl pyrophosphate (HepPP; 35 carbon atoms) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P), producing heptaprenylglyceryl phosphate (HepGP). This reaction is an ether-bond- formation step in the biosynthesis of archaea-type G1P-based membrane lipids found in Bacillales. |
| CATALYTIC ACTIVITY | Reaction=sn-glycerol 1-phosphate + all-trans-heptaprenyl diphosphate = 3-heptaprenyl-sn-glycero-1-phosphate + diphosphate; Xref=Rhea:RHEA:33495, ChEBI:CHEBI:33019, ChEBI:CHEBI:57685, ChEBI:CHEBI:58206, ChEBI:CHEBI:64781; EC=2.5.1.n9; |
| PATHWAY | Membrane lipid metabolism; glycerophospholipid metabolism. |
| SUBUNIT | Homodimer. |
| end case | |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| case <FTGroup:1> | |
| SIMILARITY | Belongs to the GGGP/HepGP synthase family. Group I subfamily. |
| else case <FTGroup:2> | |
| SIMILARITY | Belongs to the GGGP/HepGP synthase family. Group II subfamily. |
| else | |
| SIMILARITY | Belongs to the GGGP/HepGP synthase family. |
| end case | |
Keywords
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| case <OC:Archaea> | |
| Cytoplasm | |
| end case | |
| Lipid biosynthesis | |
| Lipid metabolism | |
| Magnesium | |
| Metal-binding | |
| Phospholipid biosynthesis | |
| Phospholipid metabolism | |
| Transferase | |
Gene Ontology
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| case <OCellular component:Archaea> or <OC:Bacteroidota> | |
| GO:0047294; Molecular function:phosphoglycerol geranylgeranyltransferase activity | |
| else case <OCellular component:Bacillales> | |
| GO:0004659; Molecular function:prenyltransferase activity | |
| end case | |
| GO:0000287; Molecular function:magnesium ion binding | |
| GO:0046474; Biological process:glycerophospholipid biosynthetic process | |
| case <OCellular component:Archaea> | |
| GO:0005737; Cellular component:cytoplasm | |
| end case | |
Cross-references
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| Pfam | PF01884; PcrB; 1; |
| NCBIfam | TIGR01768; GGGP-family; 1; |
| NCBIfam | TIGR01769; GGGP; 0-1; |
| NCBIfam | TIGR04146; GGGPS_Afulg; 0-1; |
| NCBIfam | TIGR04147; GGGPS_Halobact; 0-1; |
Features
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| From: GGGPS_ARCFU (O29844) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 165 | 170 | /ligand="sn-glycerol 1-phosphate" /ligand_id="ChEBI:CHEBI:57685" |
Y-[IVLM]-E-Y-S-G | 1 | |||||||
| BINDING | 215 | 216 | /ligand="sn-glycerol 1-phosphate" /ligand_id="ChEBI:CHEBI:57685" |
G-[ND] | 1 | |||||||
| BINDING | 13 | 13 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | ||||||||
| BINDING | 39 | 39 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
[ST] | ||||||||
| BINDING | 11 | 11 | /ligand="sn-glycerol 1-phosphate" /ligand_id="ChEBI:CHEBI:57685" |
K | 1 | |||||||
| BINDING | 195 | 195 | /ligand="sn-glycerol 1-phosphate" /ligand_id="ChEBI:CHEBI:57685" |
G | 1 | |||||||
| From: GGGPS_METTH (O26652) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 169 | 175 | /ligand="sn-glycerol 1-phosphate" /ligand_id="ChEBI:CHEBI:57685" |
[YF]-[LMFI]-[ED]-[AGS]-G-S-G | 2 | |||||||
| BINDING | 200 | 201 | /ligand="sn-glycerol 1-phosphate" /ligand_id="ChEBI:CHEBI:57685" |
G-G | 2 | |||||||
| BINDING | 222 | 223 | /ligand="sn-glycerol 1-phosphate" /ligand_id="ChEBI:CHEBI:57685" |
G-[TNS] | 2 | |||||||
Additional information
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| Size range | 210-304 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | Bacteroidota have GGGPS activity like the archaeal enzyme, but the physiological function is unknown (PubMed=24684232). Two phylogenetic subgroups (I and II) exist within the family. They use alternative amino acid residues to bind G1P (PubMed=24684232). Many Halobacteria possess two paralogs of GGGPS. |