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Annotation rule MF_00112
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General rule information [?]

Accession MF_00112
Dates 1-JUN-2001 (Created)
30-MAR-2020 (Last updated, Version 28)
Name GGGP_HepGP_synthase
Scope
Bacteria; Bacillales
Bacteria; Bacteroidetes
Archaea
Templates O29844 (GGGPS_ARCFU); B0R2Z3 (GGGPS_HALS3); Q9YEF5 (GGGPS_AERPE); O26652 (GGGPS_METTH); P0C882 (GGGPS_METTM); A6VHZ5 (GGGPS_METM7); Q980N1 (GGGPS_SACS2); Q5JDY1 (GGGPS_THEKO); Q9HJH3 (GGGPS_THEAC); O34790 (PCRB_BACSU); Q5L3C1 (PCRB_GEOKA); Q8Y6C8 (PCRB_LISMO); Q53726 (PCRB_STAA8); A5FJK8 (GGGPS_FLAJ1): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

case <OC:Archaea> or <OC:Bacteroidetes>
Identifier
GGGPS
Protein name
RecName: Full=Geranylgeranylglyceryl phosphate synthase;
Short=GGGP synthase;
Short=GGGPS;
EC 2.5.1.41;
AltName: Full=(S)-3-O-geranylgeranylglyceryl phosphate synthase;
AltName: Full=Phosphoglycerol geranylgeranyltransferase;
else case <OC:Bacillales>
Identifier
PCRB
Protein name
RecName: Full=Heptaprenylglyceryl phosphate synthase;
Short=HepGP synthase;
EC 2.5.1.n9;
AltName: Full=Glycerol-1-phosphate heptaprenyltransferase;
Gene name
pcrB
end case

Comments [?]

case <OC:Archaea>
Function Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P). This reaction is the first ether-bond-formation step in the biosynthesis of archaeal membrane lipids.
Catalytic activity RHEA:23404: (2E,6E,10E)-geranylgeranyl diphosphate + sn-glycerol 1-phosphate = diphosphate + sn-3-O-(geranylgeranyl)glycerol 1-phosphate
EC 2.5.1.41
Pathway Membrane lipid metabolism; glycerophospholipid metabolism.
Subcellular location Cytoplasm.
else case <OC:Bacteroidetes>
Function Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P).
Catalytic activity RHEA:23404: (2E,6E,10E)-geranylgeranyl diphosphate + sn-glycerol 1-phosphate = diphosphate + sn-3-O-(geranylgeranyl)glycerol 1-phosphate
EC 2.5.1.41
else case <OC:Bacillales>
Function Prenyltransferase that catalyzes in vivo the transfer of the heptaprenyl moiety of heptaprenyl pyrophosphate (HepPP; 35 carbon atoms) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P), producing heptaprenylglyceryl phosphate (HepGP). This reaction is an ether-bond-formation step in the biosynthesis of archaea-type G1P-based membrane lipids found in Bacillales.
Catalytic activity RHEA:33495: all-trans-heptaprenyl diphosphate + sn-glycerol 1-phosphate = 3-heptaprenyl-sn-glycero-1-phosphate + diphosphate
EC 2.5.1.n9
Pathway Membrane lipid metabolism; glycerophospholipid metabolism.
Subunit Homodimer.
end case
Cofactor Mg(2+)
case <FTGroup:1>
Similarity Belongs to the GGGP/HepGP synthase family. Group I subfamily.
else case <FTGroup:2>
Similarity Belongs to the GGGP/HepGP synthase family. Group II subfamily.
else
Similarity Belongs to the GGGP/HepGP synthase family.
end case

Keywords [?]

case <OC:Archaea>
end case

Gene Ontology [?]

case <OC:Archaea> or <OC:Bacteroidetes>
GO:0047294; Molecular function: phosphoglycerol geranylgeranyltransferase activity.
else case <OC:Bacillales>
GO:0002094; Molecular function: polyprenyltransferase activity.
end case
GO:0000287; Molecular function: magnesium ion binding.
GO:0046474; Biological process: glycerophospholipid biosynthetic process.
case <OC:Archaea>
GO:0005737; Cellular component: cytoplasm.
end case

Cross-references [?]

Pfam PF01884; PcrB; 1;
TIGRFAMs TIGR01768; GGGP-family; 1;
TIGR01769; GGGP; 0-1;
TIGR04146; GGGPS_Afulg; 0-1;
TIGR04147; GGGPS_Halobact; 0-1;

Features [?]

From: GGGPS_ARCFU (O29844)
Key     From     To       Description   Tag   Condition   FTGroup
REGION (Optional)     165     170       Glycerol-1-phosphate binding     Y-[IVLM]-E-Y-S-G   1
REGION (Optional)     215     216       Glycerol-1-phosphate binding     G-[ND]   1
METAL     13     13       Magnesium     D  
METAL     39     39       Magnesium     [ST]  
BINDING (Optional)     11     11       Glycerol-1-phosphate     K   1
BINDING (Optional)     195     195       Glycerol-1-phosphate; via amide nitrogen     G   1
From: GGGPS_METTH (O26652)
REGION (Optional)     169     175       Glycerol-1-phosphate binding     [YF]-[LMFI]-[ED]-[AGS]-G-S-G   2
REGION (Optional)     200     201       Glycerol-1-phosphate binding     G-G   2
REGION (Optional)     222     223       Glycerol-1-phosphate binding     G-[TNS]   2

Additional information [?]

Size range 210-304 amino acids
Related rules None
Fusion None
Comments Bacteroidetes have GGGPS activity like the archaeal enzyme, but the physiological function is unknown (PubMed=24684232). Two phylogenetic subgroups (I and II) exist within the family. They use alternative amino acid residues to bind G1P (PubMed=24684232). Many Halobacteria possess two paralogs of GGGPS.