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HAMAP rule MF_00116

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General rule information [?]

Accession MF_00116
Dates 1-JUN-2001 (Created)
25-JAN-2024 (Last updated, Version 33)
Name dUTPase_bact
Scope(s) Bacteria
Template(s) P06968 (DUT_ECOLI); P9WNS5 (DUT_MYCTU); [ Recover all ]
Triggered by HAMAP; MF_00116 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier DUT
Protein name RecName: Full=Deoxyuridine 5'-triphosphate nucleotidohydrolase;
AltName: Full=dUTP pyrophosphatase;
Gene name Name=dut;

Comments [?]

FUNCTIONThis enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
CATALYTIC ACTIVITY Reaction=dUTP + H2O = diphosphate + dUMP + H(+); Xref=Rhea:RHEA:10248, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:61555, ChEBI:CHEBI:246422; EC=;
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
PATHWAYPyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.
case <OC:Escherichia> or <OC:Mycobacterium>
end case
SIMILARITYBelongs to the dUTPase family.

Keywords [?]

Gene Ontology [?]

GO:0004170; Molecular function:dUTP diphosphatase activity
GO:0000287; Molecular function:magnesium ion binding
GO:0006226; Biological process:dUMP biosynthetic process

Cross-references [?]

Pfam PF00692; dUTPase; 1;
NCBIfam TIGR00576; Dut; 1;

Features [?]

From: DUT_ECOLI (P06968)
Key From To Description Tag Condition FTGroup
BINDING 71 73 /ligand="substrate" R-S-[GS]
BINDING 88 90 /ligand="substrate" [TVL]-[IV]-D
BINDING 84 84 /ligand="substrate" [NQ]
case not <FT:2>
BINDING 90 90 /ligand="substrate" D
end case
BINDING 96 96 /ligand="substrate" [KM]

Additional information [?]

Size range 139-185 amino acids
Related rules None
Fusion Nter: None Cter: None

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