HAMAP rule MF_00120
General rule information
[?]
| PURL | https://purl.expasy.org/hamap/rule/MF_00120 |
| Accession | MF_00120 |
| Dates | 28-FEB-2005 (Created)
02-SEP-2024 (Last updated, Version 18) |
| Name | GatA |
| Scope(s) |
Bacteria Archaea |
| Template(s) | O06491; Q9LCX3; [ Recover all ] |
| Triggered by |
case c? <OC:Bacteria> or <OC:Archaea>
HAMAP; MF_00120 (Get profile general information and statistics) end case
|
Propagated annotation
[?]
Identifier, protein and gene names
[?]
| Identifier | GATA |
| Protein name | RecName: Full=Glutamyl-tRNA(Gln) amidotransferase subunit A; Short=Glu-ADT subunit A; EC=6.3.5.7; |
| Gene name | Name=gatA; |
Comments
[?]
| FUNCTION | Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu- tRNA(Gln). |
| CATALYTIC ACTIVITY | Reaction=L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl- tRNA(Gln) + L-glutamate + ADP + phosphate + H(+); Xref=Rhea:RHEA:17521, Rhea:RHEA-COMP:9681, Rhea:RHEA-COMP:9684, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58359, ChEBI:CHEBI:78520, ChEBI:CHEBI:78521, ChEBI:CHEBI:456216; EC=6.3.5.7; |
| SUBUNIT | Heterotrimer of A, B and C subunits. |
| SIMILARITY | Belongs to the amidase family. GatA subfamily. |
Keywords
[?]
Gene Ontology
[?]
| GO:0050567; Molecular function:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity |
| GO:0006412; Biological process:translation |
Cross-references
[?]
Features
[?]
| From: GATA_BACSU (O06491) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 79 | 79 | /note="Charge relay system" | K | ||||||||
| ACT_SITE | 154 | 154 | /note="Charge relay system" | S | ||||||||
| ACT_SITE | 178 | 178 | /note="Acyl-ester intermediate" | S | ||||||||
Additional information
[?]
| Size range | 423-524 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | There is a possible divergent gatA in ARCFU: AF1954 |