HAMAP rule MF_00126
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_00126 |
| Accession | MF_00126 |
| Dates | 1-JUN-2001 (Created)
2-SEP-2024 (Last updated, Version 32) |
| Name | Gln_tRNA_synth |
| Scope(s) |
Bacteria |
| Template(s) | P00962 (SYQ_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_00126 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | SYQ |
| Protein name | RecName: Full=Glutamine--tRNA ligase; EC=6.1.1.18; AltName: Full=Glutaminyl-tRNA synthetase; Short=GlnRS; |
| Gene name | Name=glnS; |
Comments
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| CATALYTIC ACTIVITY | Reaction=tRNA(Gln) + L-glutamine + ATP = L-glutaminyl-tRNA(Gln) + AMP + diphosphate; Xref=Rhea:RHEA:20121, Rhea:RHEA-COMP:9662, Rhea:RHEA-COMP:9681, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:58359, ChEBI:CHEBI:78442, ChEBI:CHEBI:78521, ChEBI:CHEBI:456215; EC=6.1.1.18; |
| SUBUNIT | Monomer. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the class-I aminoacyl-tRNA synthetase family. |
Keywords
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Gene Ontology
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| GO:0005524; Molecular function:ATP binding |
| GO:0004819; Molecular function:glutamine-tRNA ligase activity |
| GO:0006424; Biological process:glutamyl-tRNA aminoacylation |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| Pfam | PF00749; tRNA-synt_1c; 1; |
| PRINTS | PR00987; TRNASYNTHGLU; 1; |
| NCBIfam | TIGR00440; glnS; 1; |
| PROSITE | PS00178; AA_TRNA_LIGASE_I; 1; |
Features
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| From: SYQ_ECOLI (P00962) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 35 | 37 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[ED]-P-N | ||||||||
| BINDING | 41 | 47 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
H-[IL]-G-H-[AS]-K-[SA] | ||||||||
| BINDING | 261 | 262 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
R-L | ||||||||
| BINDING | 269 | 271 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[MVL]-S-K | ||||||||
| REGION | 317 | 324 | /note="Interaction with tRNA" | T-K-Q-D-N-T-I-E | ||||||||
| MOTIF | 34 | 44 | /note="'HIGH' region" | P-[ED]-P-[NS]-G-[YF]-x-H-[LI]-G-H | ||||||||
| MOTIF | 268 | 272 | /note="'KMSKS' region" | [LVI]-[LMT]-S-K-R | ||||||||
| BINDING | 67 | 67 | /ligand="L-glutamine" /ligand_id="ChEBI:CHEBI:58359" |
D | ||||||||
| BINDING | 212 | 212 | /ligand="L-glutamine" /ligand_id="ChEBI:CHEBI:58359" |
Y | ||||||||
| BINDING | 231 | 231 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
T | ||||||||
Additional information
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| Size range | 552-636 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: MF_00121 (gatB) |
| Comments | Partial gatB like C-terminal domain in DEIRA. Possible wrong start site in DEIRA. |