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Annotation rule MF_00126
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General rule information [?]

Accession MF_00126
Dates 1-JUN-2001 (Created)
5-AUG-2019 (Last updated, Version 27)
Name Gln_tRNA_synth
Scope
Bacteria
Template P00962 (SYQ_ECOLI)

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
SYQ
Protein name
RecName: Full=Glutamine--tRNA ligase;
EC=6.1.1.18;
AltName: Full=Glutaminyl-tRNA synthetase;
Short=GlnRS;
Gene name
glnS

Comments [?]

Catalytic activity Reaction=ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln); Xref=Rhea:RHEA:20121, Rhea:RHEA-COMP:9662, Rhea:RHEA-COMP:9681, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:58359, ChEBI:CHEBI:78442, ChEBI:CHEBI:78521, ChEBI:CHEBI:456215; EC=6.1.1.18;.
Subunit Monomer.
Subcellular location Cytoplasm.
Similarity Belongs to the class-I aminoacyl-tRNA synthetase family.

Keywords [?]


Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0004819; Molecular function: glutamine-tRNA ligase activity.
GO:0006424; Biological process: glutamyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00749; tRNA-synt_1c; 1;
PRINTS PR00987; TRNASYNTHGLU; 1;
TIGRFAMs TIGR00440; glnS; 1;
PROSITE PS00178; AA_TRNA_LIGASE_I; 1;

Features [?]

From: SYQ_ECOLI (P00962)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND (Optional)     35     37       ATP     [ED]-P-N  
NP_BIND (Optional)     41     47       ATP     H-[IL]-G-H-[AS]-K-[SA]  
NP_BIND (Optional)     261     262       ATP     R-L  
NP_BIND (Optional)     269     271       ATP     [MVL]-S-K  
REGION (Optional)     317     324       Interaction with tRNA     T-K-Q-D-N-T-I-E  
MOTIF     34     44       'HIGH' region     P-[ED]-P-[NS]-G-[YF]-x-H-[LI]-G-H  
MOTIF     268     272       'KMSKS' region     [LVI]-[LMT]-S-K-R  
BINDING (Optional)     67     67       L-glutamine     D  
BINDING (Optional)     212     212       L-glutamine     Y  
BINDING (Optional)     231     231       ATP     T  

Additional information [?]

Size range 552-636 amino acids
Related rules None
Fusion Nter: None; Cter: MF_00121 (gatB)
Comments Partial gatB like C-terminal domain in DEIRA. Possible wrong start site in DEIRA.