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Annotation rule MF_00140 |
Accession | MF_00140 |
Dates | 1-JUN-2001 (Created) 18-NOV-2019 (Last updated, Version 38) |
Name | Trp_tRNA_synth |
Scope | Bacteria
Archaea |
Templates | P00953 (SYW_GEOSE); P21656 (SYW_BACSU); P00954 (SYW_ECOLI); Q9RVD6 (SYW2_DEIRA); Q58810 (SYW_METJA): [Recover all] |
Triggered by |
Triggered by |
Identifier |
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Protein name |
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Gene name |
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Function | Catalyzes the attachment of tryptophan to tRNA(Trp). |
Catalytic activity | RHEA:24080: ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + H(+) + L-tryptophanyl-tRNA(Trp)
EC 6.1.1.2 |
Subunit | Homodimer. |
Subcellular location | Cytoplasm. |
Similarity | Belongs to the class-I aminoacyl-tRNA synthetase family. |
Pfam | PF00579; tRNA-synt_1b; 1; |
PRINTS | PR01039; TRNASYNTHTRP; 1; |
TIGRFAMs | TIGR00233; TrpS; 1; |
PROSITE | PS00178; AA_TRNA_LIGASE_I; 1; |
From: SYW_GEOSE (P00953) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
NP_BIND | 9 | 11 | ATP | [QTRK]-[PATS]-x(0,1)-[ST] | ||||||||
NP_BIND | 17 | 18 | ATP | G-[NH] | ||||||||
NP_BIND | 144 | 146 | ATP | G-x-D | ||||||||
NP_BIND | 192 | 196 | ATP | K-M-[SG]-K-S | ||||||||
MOTIF | 10 | 18 | 'HIGH' region | [PATS]-x(0,1)-[STA]-[GDN]-x-[ILVFYQP]-[HST]-[ILW]-G-[NH] | ||||||||
MOTIF | 192 | 196 | 'KMSKS' region | K-M-[SG]-K-S | ||||||||
BINDING | 132 | 132 | L-tryptophan | D | ||||||||
BINDING | 183 | 183 | ATP; via amide nitrogen and carbonyl oxygen | [VIL] |
From: SYW_METJA (Q58810) | ||||||||||||
MOTIF | 75 | 83 | 'HIGH' region | P-[ST]-x(2)-[MVFP]-H-[LIF]-G-[HN] | ||||||||
MOTIF | 255 | 259 | 'KMSKS' region | K-M-S-[SA]-[SN] |
Size range | 319-451 amino acids |
Size range | 364-437 amino acids |
Related rules | None |
Fusion | None |
Comments | Weird insertions in AQUAE, RALSO and METMA; not shown in alignment. DEIRA has a second tryptophanyl-tRNA synthetase (DR1093), which shows a weaker activity and whose role in protein biosynthesis has not been proven. It is probably involved in other processes. |