HAMAP rule MF_00140
General rule information
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| Accession | MF_00140 |
| Dates | 1-JUN-2001 (Created)
2-SEP-2024 (Last updated, Version 43) |
| Name | Trp_tRNA_synth |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P00953 (SYW_GEOSE); P21656 (SYW_BACSU); P00954 (SYW_ECOLI); Q9RVD6 (SYW2_DEIRA); Q58810 (SYW_METJA); [ Recover all ] |
| Triggered by |
case c? <OC:Bacteria>
HAMAP; MF_00140_B (Get profile general information and statistics) end case
case c? <OC:Archaea>
HAMAP; MF_00140_A (Get profile general information and statistics) end case
|
Propagated annotation
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Identifier, protein and gene names
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| Identifier | SYW |
| Protein name | RecName: Full=Tryptophan--tRNA ligase; EC=6.1.1.2; AltName: Full=Tryptophanyl-tRNA synthetase; Short=TrpRS; |
| Gene name | Name=trpS; |
Comments
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| FUNCTION | Catalyzes the attachment of tryptophan to tRNA(Trp). |
| CATALYTIC ACTIVITY | Reaction=tRNA(Trp) + L-tryptophan + ATP = L-tryptophanyl-tRNA(Trp) + AMP + diphosphate + H(+); Xref=Rhea:RHEA:24080, Rhea:RHEA-COMP:9671, Rhea:RHEA-COMP:9705, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57912, ChEBI:CHEBI:78442, ChEBI:CHEBI:78535, ChEBI:CHEBI:456215; EC=6.1.1.2; |
| case <OC:Bacteria> | |
| SUBUNIT | Homodimer. |
| end case | |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the class-I aminoacyl-tRNA synthetase family. |
Keywords
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Gene Ontology
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| GO:0005524; Molecular function:ATP binding |
| GO:0004830; Molecular function:tryptophan-tRNA ligase activity |
| GO:0006436; Biological process:tryptophanyl-tRNA aminoacylation |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| Pfam | PF00579; tRNA-synt_1b; 1; |
| PRINTS | PR01039; TRNASYNTHTRP; 1; |
| NCBIfam | TIGR00233; TrpS; 1; |
| PROSITE | PS00178; AA_TRNA_LIGASE_I; 1; |
Features
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| From: SYW_GEOSE (P00953) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| case <OC:Bacteria> | ||||||||||||
| BINDING | 9 | 11 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[QTRK]-[PATS]-x(0,1)-[ST] | ||||||||
| BINDING | 17 | 18 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G-[NH] | ||||||||
| BINDING | 144 | 146 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G-x-D | ||||||||
| BINDING | 192 | 196 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
K-M-[SG]-K-S | ||||||||
| MOTIF | 10 | 18 | /note="'HIGH' region" | [PATS]-x(0,1)-[STA]-[GDN]-x-[ILVFYQP]-[HST]-[ILW]-G-[NH] | ||||||||
| MOTIF | 192 | 196 | /note="'KMSKS' region" | K-M-[SG]-K-S | ||||||||
| BINDING | 132 | 132 | /ligand="L-tryptophan" /ligand_id="ChEBI:CHEBI:57912" |
D | ||||||||
| BINDING | 183 | 183 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[VIL] | ||||||||
| end case | ||||||||||||
| From: SYW_METJA (Q58810) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| case <OC:Archaea> | ||||||||||||
| MOTIF | 75 | 83 | /note="'HIGH' region" | P-[ST]-x(2)-[MVFP]-H-[LIF]-G-[HN] | ||||||||
| MOTIF | 255 | 259 | /note="'KMSKS' region" | K-M-S-[SA]-[SN] | ||||||||
| end case | ||||||||||||
Additional information
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| Size range | 364-437 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | Weird insertions in AQUAE, RALN1 and METMA; not shown in alignment. DEIRA has a second tryptophanyl-tRNA synthetase (DR1093), which shows a weaker activity and whose role in protein biosynthesis has not been proven. It is probably involved in other processes. |