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| Annotation rule MF_00140 |
General rule information
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| Accession | MF_00140 |
| Dates | 1-JUN-2001 (Created) 18-NOV-2019 (Last updated, Version 38) |
| Name | Trp_tRNA_synth |
| Scope | Bacteria
Archaea |
| Templates | P00953 (SYW_GEOSE); P21656 (SYW_BACSU); P00954 (SYW_ECOLI); Q9RVD6 (SYW2_DEIRA); Q58810 (SYW_METJA): [Recover all] |
case <OC:Bacteria>
| Triggered by |
end case
case <OC:Archaea>
| Triggered by |
end case
Propagated annotation
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Identifier, protein and gene names
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| Identifier |
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| Protein name |
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| Gene name |
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Comments
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| Function | Catalyzes the attachment of tryptophan to tRNA(Trp). |
| Catalytic activity | RHEA:24080: ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + H(+) + L-tryptophanyl-tRNA(Trp)
EC 6.1.1.2 |
case <OC:Bacteria>
| Subunit | Homodimer. |
end case
| Subcellular location | Cytoplasm. |
| Similarity | Belongs to the class-I aminoacyl-tRNA synthetase family. |
Keywords
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Gene Ontology
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GO:0005524; Molecular function: ATP binding.
GO:0004830; Molecular function: tryptophan-tRNA ligase activity.
GO:0006436; Biological process: tryptophanyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.
GO:0004830; Molecular function: tryptophan-tRNA ligase activity.
GO:0006436; Biological process: tryptophanyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.
Cross-references
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| Pfam | PF00579; tRNA-synt_1b; 1; |
| PRINTS | PR01039; TRNASYNTHTRP; 1; |
| TIGRFAMs | TIGR00233; TrpS; 1; |
| PROSITE | PS00178; AA_TRNA_LIGASE_I; 1; |
Features
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case <OC:Bacteria>
| From: SYW_GEOSE (P00953) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| NP_BIND | 9 | 11 | ATP | [QTRK]-[PATS]-x(0,1)-[ST] | ||||||||
| NP_BIND | 17 | 18 | ATP | G-[NH] | ||||||||
| NP_BIND | 144 | 146 | ATP | G-x-D | ||||||||
| NP_BIND | 192 | 196 | ATP | K-M-[SG]-K-S | ||||||||
| MOTIF | 10 | 18 | 'HIGH' region | [PATS]-x(0,1)-[STA]-[GDN]-x-[ILVFYQP]-[HST]-[ILW]-G-[NH] | ||||||||
| MOTIF | 192 | 196 | 'KMSKS' region | K-M-[SG]-K-S | ||||||||
| BINDING | 132 | 132 | L-tryptophan | D | ||||||||
| BINDING | 183 | 183 | ATP; via amide nitrogen and carbonyl oxygen | [VIL] | ||||||||
end case
case <OC:Archaea>
| From: SYW_METJA (Q58810) | ||||||||||||
| MOTIF | 75 | 83 | 'HIGH' region | P-[ST]-x(2)-[MVFP]-H-[LIF]-G-[HN] | ||||||||
| MOTIF | 255 | 259 | 'KMSKS' region | K-M-S-[SA]-[SN] | ||||||||
end case
Additional information
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case <OC:Bacteria>
| Size range | 319-451 amino acids |
end case
case <OC:Archaea>
| Size range | 364-437 amino acids |
end case
| Related rules | None |
| Fusion | None |
| Comments | Weird insertions in AQUAE, RALSO and METMA; not shown in alignment. DEIRA has a second tryptophanyl-tRNA synthetase (DR1093), which shows a weaker activity and whose role in protein biosynthesis has not been proven. It is probably involved in other processes. |