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Annotation rule MF_00140
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General rule information [?]

Accession MF_00140
Dates 1-JUN-2001 (Created)
18-NOV-2019 (Last updated, Version 38)
Name Trp_tRNA_synth
Scope
Bacteria
Archaea
Templates P00953 (SYW_GEOSE); P21656 (SYW_BACSU); P00954 (SYW_ECOLI); Q9RVD6 (SYW2_DEIRA); Q58810 (SYW_METJA): [Recover all]
case <OC:Bacteria>
end case

case <OC:Archaea>
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
SYW
Protein name
RecName: Full=Tryptophan--tRNA ligase;
EC 6.1.1.2;
AltName: Full=Tryptophanyl-tRNA synthetase;
Short=TrpRS;
Gene name
trpS

Comments [?]

Function Catalyzes the attachment of tryptophan to tRNA(Trp).
Catalytic activity RHEA:24080: ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + H(+) + L-tryptophanyl-tRNA(Trp)
EC 6.1.1.2
case <OC:Bacteria>
Subunit Homodimer.
end case
Subcellular location Cytoplasm.
Similarity Belongs to the class-I aminoacyl-tRNA synthetase family.

Keywords [?]


Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0004830; Molecular function: tryptophan-tRNA ligase activity.
GO:0006436; Biological process: tryptophanyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00579; tRNA-synt_1b; 1;
PRINTS PR01039; TRNASYNTHTRP; 1;
TIGRFAMs TIGR00233; TrpS; 1;
PROSITE PS00178; AA_TRNA_LIGASE_I; 1;

Features [?]

case <OC:Bacteria>
From: SYW_GEOSE (P00953)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     9     11       ATP     [QTRK]-[PATS]-x(0,1)-[ST]  
NP_BIND     17     18       ATP     G-[NH]  
NP_BIND     144     146       ATP     G-x-D  
NP_BIND     192     196       ATP     K-M-[SG]-K-S  
MOTIF     10     18       'HIGH' region     [PATS]-x(0,1)-[STA]-[GDN]-x-[ILVFYQP]-[HST]-[ILW]-G-[NH]  
MOTIF     192     196       'KMSKS' region     K-M-[SG]-K-S  
BINDING     132     132       L-tryptophan     D  
BINDING     183     183       ATP; via amide nitrogen and carbonyl oxygen     [VIL]  
end case
case <OC:Archaea>
From: SYW_METJA (Q58810)
MOTIF     75     83       'HIGH' region     P-[ST]-x(2)-[MVFP]-H-[LIF]-G-[HN]  
MOTIF     255     259       'KMSKS' region     K-M-S-[SA]-[SN]  
end case

Additional information [?]

case <OC:Bacteria>
Size range 319-451 amino acids
end case
case <OC:Archaea>
Size range 364-437 amino acids
end case
Related rules None
Fusion None
Comments Weird insertions in AQUAE, RALSO and METMA; not shown in alignment. DEIRA has a second tryptophanyl-tRNA synthetase (DR1093), which shows a weaker activity and whose role in protein biosynthesis has not been proven. It is probably involved in other processes.