HAMAP rule MF_00152

General rule information [?]

Accession	MF_00152
Dates	1-JUN-2001 (Created) 1-JUN-2023 (Last updated, Version 32)

Name

Nfo

Scope

Bacteria

Archaea

Template

P0A6C1 (END4_ECOLI)

Triggered by

HAMAP; MF_00152 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

END4

Protein name

RecName:		Full=Probable endonuclease 4;
		EC 3.1.21.2;
AltName:		Full=Endodeoxyribonuclease IV;
AltName:		Full=Endonuclease IV;

Gene name

nfo

Comments [?]

Function	Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic (AP) sites, generating a 3'-hydroxyl group and a 5'-terminal sugar phosphate.
Catalytic activity	Reaction=Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products.; EC=3.1.21.2;
Cofactor	Zn(2+) Note: Binds 3 Zn(2+) ions.
Similarity	Belongs to the AP endonuclease 2 family.

Keywords [?]

Hydrolase
Nuclease
Endonuclease
DNA damage
DNA repair
Metal-binding
Zinc

Gene Ontology [?]

GO:0008833; Molecular function: deoxyribonuclease IV (phage-T4-induced) activity.
GO:0008270; Molecular function: zinc ion binding.
GO:0006281; Biological process: DNA repair.

Cross-references [?]

Pfam	PF01261; AP_endonuc_2; 1;
NCBIfam	TIGR00587; Nfo; 1;
PROSITE	PS00729; AP_NUCLEASE_F2_1; 1;
	PS00730; AP_NUCLEASE_F2_2; 1;
	PS00731; AP_NUCLEASE_F2_3; 1;
	PS51432; AP_NUCLEASE_F2_4; 1;

Features [?]

From: END4_ECOLI (P0A6C1)

Key From To Description Tag Condition FTGroup

BINDING 69 69 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1 H

BINDING 109 109 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1 H

BINDING 145 145 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1 E

BINDING 145 145 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2 E

BINDING 179 179 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2 D

BINDING 182 182 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="3 H

BINDING 216 216 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2 H

BINDING 229 229 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="3 D

BINDING 231 231 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="3 H

BINDING 261 261 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2 E

Additional information [?]

Size range	252-310 amino acids
Related rules	None
Fusion	None

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