HAMAP logo

HAMAP rule MF_00152

Send feedback

General rule information [?]

Accession MF_00152
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 32)
Name Nfo
Scope
Bacteria
Archaea
Template P0A6C1 (END4_ECOLI)

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
END4
Protein name
RecName: Full=Probable endonuclease 4;
EC 3.1.21.2;
AltName: Full=Endodeoxyribonuclease IV;
AltName: Full=Endonuclease IV;
Gene name
nfo

Comments [?]

Function Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic (AP) sites, generating a 3'-hydroxyl group and a 5'-terminal sugar phosphate.
Catalytic activity Reaction=Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products.; EC=3.1.21.2;
Cofactor Zn(2+)
Note: Binds 3 Zn(2+) ions.
Similarity Belongs to the AP endonuclease 2 family.

Keywords [?]


Gene Ontology [?]

GO:0008833; Molecular function: deoxyribonuclease IV (phage-T4-induced) activity.
GO:0008270; Molecular function: zinc ion binding.
GO:0006281; Biological process: DNA repair.

Cross-references [?]

Pfam PF01261; AP_endonuc_2; 1;
NCBIfam TIGR00587; Nfo; 1;
PROSITE PS00729; AP_NUCLEASE_F2_1; 1;
PS00730; AP_NUCLEASE_F2_2; 1;
PS00731; AP_NUCLEASE_F2_3; 1;
PS51432; AP_NUCLEASE_F2_4; 1;

Features [?]

From: END4_ECOLI (P0A6C1)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     69     69       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1     H  
BINDING     109     109       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1     H  
BINDING     145     145       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1     E  
BINDING     145     145       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2     E  
BINDING     179     179       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2     D  
BINDING     182     182       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="3     H  
BINDING     216     216       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2     H  
BINDING     229     229       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="3     D  
BINDING     231     231       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="3     H  
BINDING     261     261       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2     E  

Additional information [?]

Size range 252-310 amino acids
Related rules None
Fusion None