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Annotation rule MF_00171 |
Accession | MF_00171 |
Dates | 1-JUN-2001 (Created) 18-NOV-2019 (Last updated, Version 31) |
Name | TruA |
Scope | Bacteria
Archaea |
Template | P07649 (TRUA_ECOLI) |
Triggered by |
Identifier |
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Protein name |
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Gene name |
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Function | Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs. |
Catalytic activity | RHEA:22376: uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA
EC 5.4.99.12 |
Subunit | Homodimer. |
Similarity | Belongs to the tRNA pseudouridine synthase TruA family. |
Pfam | PF01416; PseudoU_synth_1; 1; |
TIGRFAMs | TIGR00071; HisT_truA; 1; |
PIRSF | PIRSF001430; tRNA_psdUrid_synth; 1; |
From: TRUA_ECOLI (P07649) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
REGION (Optional) | 107 | 111 | RNA binding | F-H-A-R-F | 1 | |||||||
REGION (Optional) | 168 | 172 | Interaction with tRNA | Q-C-Q-S-R | 1 | |||||||
ACT_SITE | 60 | 60 | Nucleophile | D | ||||||||
BINDING | 118 | 118 | Substrate | Y | ||||||||
SITE (Optional) | 58 | 58 | Interaction with tRNA. Important for base-flipping | R | 1 | |||||||
SITE (Optional) | 78 | 78 | Interaction with tRNA | R | 1 | |||||||
SITE (Optional) | 110 | 110 | Interaction with tRNA | R | 1 | |||||||
SITE (Optional) | 126 | 126 | Interaction with tRNA | R | 1 | |||||||
SITE (Optional) | 139 | 139 | Interaction with tRNA | F | 1 |
Size range | 240-304 amino acids |
Related rules | None |
Fusion | None |
Comments | Possible wrong start in MYCLE, MYCTU and MYCPA. Divergent LEPIN and NANEQ; not shown in alignment and not used in size range. |