HAMAP logo

HAMAP rule MF_00171

Send feedback

General rule information [?]

Accession MF_00171
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 33)
Name TruA
Scope(s) Bacteria
Template(s) P07649 (TRUA_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_00171 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier TRUA
Protein name RecName: Full=tRNA pseudouridine synthase A;
AltName: Full=tRNA pseudouridine(38-40) synthase;
AltName: Full=tRNA pseudouridylate synthase I;
AltName: Full=tRNA-uridine isomerase I;
Gene name Name=truA;

Comments [?]

FUNCTIONFormation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs.
CATALYTIC ACTIVITY Reaction=uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA; Xref=Rhea:RHEA:22376, Rhea:RHEA-COMP:10085, Rhea:RHEA-COMP:10087, ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=;
case <OC:Bacteria>
end case
SIMILARITYBelongs to the tRNA pseudouridine synthase TruA family.

Keywords [?]

Gene Ontology [?]

GO:0009982; Molecular function:pseudouridine synthase activity
GO:0031119; Biological process:tRNA pseudouridine synthesis

Cross-references [?]

Pfam PF01416; PseudoU_synth_1; 1;
NCBIfam TIGR00071; HisT_truA; 1;
PIRSF PIRSF001430; tRNA_psdUrid_synth; 1;

Features [?]

From: TRUA_ECOLI (P07649)
Key From To Description Tag Condition FTGroup
REGION 107 111 /note="RNA binding" F-H-A-R-F 1
REGION 168 172 /note="Interaction with tRNA" Q-C-Q-S-R 1
ACT_SITE 60 60 /note="Nucleophile" D
BINDING 118 118 /ligand="substrate" Y
SITE 58 58 /note="Interaction with tRNA. Important for base-flipping" R 1
SITE 78 78 /note="Interaction with tRNA" R 1
SITE 110 110 /note="Interaction with tRNA" R 1
SITE 126 126 /note="Interaction with tRNA" R 1
SITE 139 139 /note="Interaction with tRNA" F 1

Additional information [?]

Size range 240-304 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments Possible wrong start in MYCLE, MYCTU and MYCPA. Divergent LEPIN and NANEQ; not shown in alignment and not used in size range.

View rule in raw text format (no links)