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Annotation rule MF_00171
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General rule information [?]

Accession MF_00171
Dates 1-JUN-2001 (Created)
18-NOV-2019 (Last updated, Version 31)
Name TruA
Scope
Bacteria
Archaea
Template P07649 (TRUA_ECOLI)
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
TRUA
Protein name
RecName: Full=tRNA pseudouridine synthase A;
EC 5.4.99.12;
AltName: Full=tRNA pseudouridine(38-40) synthase;
AltName: Full=tRNA pseudouridylate synthase I;
AltName: Full=tRNA-uridine isomerase I;
Gene name
truA

Comments [?]

Function Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs.
Catalytic activity RHEA:22376: uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA
EC 5.4.99.12
case <OC:Bacteria>
Subunit Homodimer.
end case
Similarity Belongs to the tRNA pseudouridine synthase TruA family.

Keywords [?]

Isomerase
tRNA processing

Gene Ontology [?]

GO:0009982; Molecular function: pseudouridine synthase activity.
GO:0031119; Biological process: tRNA pseudouridine synthesis.

Cross-references [?]

Pfam PF01416; PseudoU_synth_1; 1;
TIGRFAMs TIGR00071; HisT_truA; 1;
PIRSF PIRSF001430; tRNA_psdUrid_synth; 1;

Features [?]

From: TRUA_ECOLI (P07649)
Key     From     To       Description   Tag   Condition   FTGroup
REGION (Optional)     107     111       RNA binding     F-H-A-R-F   1
REGION (Optional)     168     172       Interaction with tRNA     Q-C-Q-S-R   1
ACT_SITE     60     60       Nucleophile     D  
BINDING     118     118       Substrate     Y  
SITE (Optional)     58     58       Interaction with tRNA. Important for base-flipping     R   1
SITE (Optional)     78     78       Interaction with tRNA     R   1
SITE (Optional)     110     110       Interaction with tRNA     R   1
SITE (Optional)     126     126       Interaction with tRNA     R   1
SITE (Optional)     139     139       Interaction with tRNA     F   1

Additional information [?]

Size range 240-304 amino acids
Related rules None
Fusion None
Comments Possible wrong start in MYCLE, MYCTU and MYCPA. Divergent LEPIN and NANEQ; not shown in alignment and not used in size range.


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