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HAMAP rule MF_00172

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General rule information [?]

Accession MF_00172
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 34)
Name Meth_synth
Scope(s) Bacteria
Template(s) P25665 (METE_ECOLI); Q9X112 (METE_THEMA); Q8CWX6 (METE_STRMU); [ Recover all ]
Triggered by HAMAP; MF_00172 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier METE
Protein name RecName: Full=5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase;
                 EC=2.1.1.14;
AltName: Full=Cobalamin-independent methionine synthase;
AltName: Full=Methionine synthase, vitamin-B12 independent isozyme;
Gene name Name=metE;

Comments [?]

FUNCTIONCatalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation.
CATALYTIC ACTIVITY Reaction=5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = L- methionine + tetrahydropteroyltri-L-glutamate; Xref=Rhea:RHEA:21196, ChEBI:CHEBI:57844, ChEBI:CHEBI:58140, ChEBI:CHEBI:58199, ChEBI:CHEBI:58207; EC=2.1.1.14;
COFACTOR Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit.;
PATHWAYAmino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1.
SIMILARITYBelongs to the vitamin-B12 independent methionine synthase family.

Keywords [?]


Gene Ontology [?]

GO:0003871; Molecular function:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity
GO:0009086; Biological process:methionine biosynthetic process

Cross-references [?]

Pfam PF08267; Meth_synt_1; 1;
Pfam PF01717; Meth_synt_2; 1;
PIRSF PIRSF000382; MeTrfase_B12_ind; 1;
NCBIfam TIGR01371; Met_syn_B12ind; 1;

Features [?]

From: METE_THEMA (Q9X112)
Key From To Description Tag Condition FTGroup
BINDING 15 18 /ligand="5-methyltetrahydropteroyltri-L-glutamate"
/ligand_id="ChEBI:CHEBI:58207"
R-E-x-K
BINDING 409 411 /ligand="L-homocysteine"
/ligand_id="ChEBI:CHEBI:58199"
I-G-S
BINDING 409 411 /ligand="L-methionine"
/ligand_id="ChEBI:CHEBI:57844"
I-G-S
BINDING 493 494 /ligand="5-methyltetrahydropteroyltri-L-glutamate"
/ligand_id="ChEBI:CHEBI:58207"
R-C
ACT_SITE 672 672 /note="Proton donor" H
BINDING 618 618 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_note="catalytic"
H
BINDING 620 620 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_note="catalytic"
C
BINDING 642 642 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_note="catalytic"
E
BINDING 704 704 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_note="catalytic"
C
BINDING 104 104 /ligand="5-methyltetrahydropteroyltri-L-glutamate"
/ligand_id="ChEBI:CHEBI:58207"
[KR]
BINDING 462 462 /ligand="L-homocysteine"
/ligand_id="ChEBI:CHEBI:58199"
E
BINDING 462 462 /ligand="L-methionine"
/ligand_id="ChEBI:CHEBI:57844"
E
BINDING 539 539 /ligand="5-methyltetrahydropteroyltri-L-glutamate"
/ligand_id="ChEBI:CHEBI:58207"
W
BINDING 577 577 /ligand="L-homocysteine"
/ligand_id="ChEBI:CHEBI:58199"
D
BINDING 577 577 /ligand="L-methionine"
/ligand_id="ChEBI:CHEBI:57844"
D
BINDING 583 583 /ligand="5-methyltetrahydropteroyltri-L-glutamate"
/ligand_id="ChEBI:CHEBI:58207"
E

Additional information [?]

Size range 734-792 amino acids
Related rules MF_00288
Fusion Nter: None Cter: None



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