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HAMAP rule MF_00172

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General rule information [?]

Accession MF_00172
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 34)
Name Meth_synth
Templates P25665 (METE_ECOLI); Q9X112 (METE_THEMA); Q8CWX6 (METE_STRMU): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase;
AltName: Full=Cobalamin-independent methionine synthase;
AltName: Full=Methionine synthase, vitamin-B12 independent isozyme;
Gene name

Comments [?]

Function Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.
Catalytic activity RHEA:21196: 5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = L-methionine + tetrahydropteroyltri-L-glutamate
Cofactor Zn(2+)
Note: Binds 1 zinc ion per subunit.
Pathway Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1.
Similarity Belongs to the vitamin-B12 independent methionine synthase family.

Keywords [?]

Gene Ontology [?]

GO:0003871; Molecular function: 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity.
GO:0009086; Biological process: methionine biosynthetic process.

Cross-references [?]

Pfam PF08267; Meth_synt_1; 1;
PF01717; Meth_synt_2; 1;
PIRSF PIRSF000382; MeTrfase_B12_ind; 1;
NCBIfam TIGR01371; Met_syn_B12ind; 1;

Features [?]

From: METE_THEMA (Q9X112)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING (Optional)     15     18       /ligand="5-methyltetrahydropteroyltri-L-glutamate" /ligand_id="ChEBI:CHEBI:58207     R-E-x-K  
BINDING (Optional)     409     411       /ligand="L-homocysteine" /ligand_id="ChEBI:CHEBI:58199     I-G-S  
BINDING (Optional)     409     411       /ligand="L-methionine" /ligand_id="ChEBI:CHEBI:57844     I-G-S  
BINDING (Optional)     493     494       /ligand="5-methyltetrahydropteroyltri-L-glutamate" /ligand_id="ChEBI:CHEBI:58207     R-C  
ACT_SITE     672     672       Proton donor     H  
BINDING     618     618       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_note="catalytic     H  
BINDING     620     620       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_note="catalytic     C  
BINDING     642     642       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_note="catalytic     E  
BINDING     704     704       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_note="catalytic     C  
BINDING (Optional)     104     104       /ligand="5-methyltetrahydropteroyltri-L-glutamate" /ligand_id="ChEBI:CHEBI:58207     [KR]  
BINDING (Optional)     462     462       /ligand="L-homocysteine" /ligand_id="ChEBI:CHEBI:58199     E  
BINDING (Optional)     462     462       /ligand="L-methionine" /ligand_id="ChEBI:CHEBI:57844     E  
BINDING (Optional)     539     539       /ligand="5-methyltetrahydropteroyltri-L-glutamate" /ligand_id="ChEBI:CHEBI:58207     W  
BINDING (Optional)     577     577       /ligand="L-homocysteine" /ligand_id="ChEBI:CHEBI:58199     D  
BINDING (Optional)     577     577       /ligand="L-methionine" /ligand_id="ChEBI:CHEBI:57844     D  
BINDING (Optional)     583     583       /ligand="5-methyltetrahydropteroyltri-L-glutamate" /ligand_id="ChEBI:CHEBI:58207     E  

Additional information [?]

Size range 734-792 amino acids
Related rules MF_00288 (METE)
Fusion None