HAMAP rule MF_00172

General rule information [?]

Accession	MF_00172
Dates	1-JUN-2001 (Created) 1-JUN-2023 (Last updated, Version 34)

Name	Meth_synth

Scope

Bacteria

Templates

P25665 (METE_ECOLI); Q9X112 (METE_THEMA); Q8CWX6 (METE_STRMU): [Recover all]

Triggered by

HAMAP; MF_00172 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

METE

Protein name

RecName:		Full=5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase;
		EC 2.1.1.14;
AltName:		Full=Cobalamin-independent methionine synthase;
AltName:		Full=Methionine synthase, vitamin-B12 independent isozyme;

Gene name

metE

Comments [?]

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.
Catalytic activity	RHEA:21196: 5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = L-methionine + tetrahydropteroyltri-L-glutamate EC 2.1.1.14
Cofactor	Zn(2+) Note: Binds 1 zinc ion per subunit.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1.
Similarity	Belongs to the vitamin-B12 independent methionine synthase family.

Keywords [?]

Amino-acid biosynthesis
Metal-binding
Methionine biosynthesis
Methyltransferase
Repeat
Transferase
Zinc

Gene Ontology [?]

GO:0003871; Molecular function: 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity.
GO:0009086; Biological process: methionine biosynthetic process.

Cross-references [?]

Pfam	PF08267; Meth_synt_1; 1;
	PF01717; Meth_synt_2; 1;
PIRSF	PIRSF000382; MeTrfase_B12_ind; 1;
NCBIfam	TIGR01371; Met_syn_B12ind; 1;

Features [?]

From: METE_THEMA (Q9X112)

Key From To Description Tag Condition FTGroup

BINDING (Optional) 15 18 /ligand="5-methyltetrahydropteroyltri-L-glutamate" /ligand_id="ChEBI:CHEBI:58207 R-E-x-K

BINDING (Optional) 409 411 /ligand="L-homocysteine" /ligand_id="ChEBI:CHEBI:58199 I-G-S

BINDING (Optional) 409 411 /ligand="L-methionine" /ligand_id="ChEBI:CHEBI:57844 I-G-S

BINDING (Optional) 493 494 /ligand="5-methyltetrahydropteroyltri-L-glutamate" /ligand_id="ChEBI:CHEBI:58207 R-C

ACT_SITE 672 672 Proton donor H

BINDING 618 618 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_note="catalytic H

BINDING 620 620 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_note="catalytic C

BINDING 642 642 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_note="catalytic E

BINDING 704 704 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_note="catalytic C

BINDING (Optional) 104 104 /ligand="5-methyltetrahydropteroyltri-L-glutamate" /ligand_id="ChEBI:CHEBI:58207 [KR]

BINDING (Optional) 462 462 /ligand="L-homocysteine" /ligand_id="ChEBI:CHEBI:58199 E

BINDING (Optional) 462 462 /ligand="L-methionine" /ligand_id="ChEBI:CHEBI:57844 E

BINDING (Optional) 539 539 /ligand="5-methyltetrahydropteroyltri-L-glutamate" /ligand_id="ChEBI:CHEBI:58207 W

BINDING (Optional) 577 577 /ligand="L-homocysteine" /ligand_id="ChEBI:CHEBI:58199 D

BINDING (Optional) 577 577 /ligand="L-methionine" /ligand_id="ChEBI:CHEBI:57844 D

BINDING (Optional) 583 583 /ligand="5-methyltetrahydropteroyltri-L-glutamate" /ligand_id="ChEBI:CHEBI:58207 E

Additional information [?]

Size range	734-792 amino acids
Related rules	MF_00288 (METE)
Fusion	None

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