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HAMAP rule MF_00174

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General rule information [?]

Accession MF_00174
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 27)
Name EF_P_modif_A
Scope(s) Bacteria
Gammaproteobacteria
Template(s) P0A8N7 (EPMA_ECOLI); Q9ZJ12 (EPMA_SALTY); [ Recover all ]
Triggered by HAMAP; MF_00174 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier EPMA
Protein name RecName: Full=Elongation factor P--(R)-beta-lysine ligase;
                 Short=EF-P--(R)-beta-lysine ligase;
                 EC=6.3.1.-;
AltName: Full=EF-P-lysine lysyltransferase;
AltName: Full=EF-P post-translational modification enzyme A;
Gene name Name=epmA;

Comments [?]

case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella>
FUNCTIONWith EpmB is involved in the beta-lysylation step of the post-translational modification of translation elongation factor P (EF- P) on 'Lys-34'. Catalyzes the ATP-dependent activation of (R)-beta- lysine produced by EpmB, forming a lysyl-adenylate, from which the beta-lysyl moiety is then transferred to the epsilon-amino group of EF- P 'Lys-34'.
else
FUNCTIONWith EpmB is involved in the beta-lysylation step of the post-translational modification of translation elongation factor P (EF- P). Catalyzes the ATP-dependent activation of (R)-beta-lysine produced by EpmB, forming a lysyl-adenylate, from which the beta-lysyl moiety is then transferred to the epsilon-amino group of a conserved specific lysine residue in EF-P.
end case
SUBUNITHomodimer.
SIMILARITYBelongs to the class-II aminoacyl-tRNA synthetase family. EpmA subfamily.

Keywords [?]


Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
GO:0016880; Molecular function:acid-ammonia (or amide) ligase activity
GO:0071915; Biological process:protein-lysine lysylation

Cross-references [?]

Pfam PF00152; tRNA-synt_2; 1;
PRINTS PR00982; TRNASYNTHLYS; 1;
NCBIfam TIGR00462; GenX; 1;
PROSITE PS50862; AA_TRNA_LIGASE_II; 1;

Features [?]

From: EPMA_ECOLI (P0A8N7)
Key From To Description Tag Condition FTGroup
BINDING 100 102 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
R-x-[EQKN]
BINDING 244 245 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
E-L
BINDING 76 78 /ligand="substrate" S-x-E
BINDING 109 109 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
N
BINDING 118 118 /ligand="substrate" Y
BINDING 251 251 /ligand="substrate" E
BINDING 300 300 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
G

Additional information [?]

Size range 293-327 amino acids
Related rules None
Fusion Nter: None Cter: None



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