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Annotation rule MF_00181
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General rule information [?]

Accession MF_00181
Dates 1-JUN-2001 (Created)
18-NOV-2019 (Last updated, Version 34)
Name Cytosol_peptidase_M17
Scope
Bacteria
Archaea
Templates P68767 (AMPA_ECOLI); P27888 (AMPA_RICPR): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
AMPA
Protein name
RecName: Full=Probable cytosol aminopeptidase;
EC 3.4.11.1;
AltName: Full=Leucine aminopeptidase;
Short=LAP;
EC 3.4.11.10;
AltName: Full=Leucyl aminopeptidase;
Gene name
pepA

Comments [?]

Function Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.
Catalytic activity Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1;
Reaction=Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.; EC=3.4.11.10;
Cofactor Mn(2+)
Note: Binds 2 manganese ions per subunit.
Subcellular location Cytoplasm.
Similarity Belongs to the peptidase M17 family.

Keywords [?]

Cytoplasm
Hydrolase
Protease
Aminopeptidase
Manganese
Metal-binding

Gene Ontology [?]

GO:0004177; Molecular function: aminopeptidase activity.
GO:0008235; Molecular function: metalloexopeptidase activity.
GO:0030145; Molecular function: manganese ion binding.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00883; Peptidase_M17; 1;
PRINTS PR00481; LAMNOPPTDASE; 1;
PROSITE PS00631; CYTOSOL_AP; 1;

Features [?]

From: AMPA_ECOLI (P68767)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     282     282             K  
ACT_SITE     356     356             R  
METAL     270     270       Manganese 2     K  
METAL     275     275       Manganese 1     D  
METAL     275     275       Manganese 2     D  
METAL     293     293       Manganese 2     D  
METAL     352     352       Manganese 1     D  
METAL     354     354       Manganese 1     E  
METAL     354     354       Manganese 2     E  

Additional information [?]

Size range 445-524 amino acids
Related rules MF_00504 (PEPB)
Fusion None
Comments Possible wrong C-terminal sequence in MYCSA


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