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HAMAP rule MF_00185

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General rule information [?]

Accession MF_00185
Dates 1-JUN-2001 (Created)
19-NOV-2022 (Last updated, Version 33)
Name IPP_trans
Scope
Bacteria
Template P16384 (MIAA_ECOLI)
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
MIAA
Protein name
RecName: Full=tRNA dimethylallyltransferase;
EC 2.5.1.75;
AltName: Full=Dimethylallyl diphosphate:tRNA dimethylallyltransferase;
Short=DMAPP:tRNA dimethylallyltransferase;
Short=DMATase;
AltName: Full=Isopentenyl-diphosphate:tRNA isopentenyltransferase;
Short=IPP transferase;
Short=IPPT;
Short=IPTase;
Gene name
miaA

Comments [?]

Function Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A).
Catalytic activity RHEA:26482: adenosine(37) in tRNA + dimethylallyl diphosphate = diphosphate + N(6)-dimethylallyladenosine(37) in tRNA
EC 2.5.1.75
Cofactor Mg(2+)
Subunit Monomer.
Similarity Belongs to the IPP transferase family.

Keywords [?]

ATP-binding
Magnesium
Nucleotide-binding
Transferase
tRNA processing

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0052381; Molecular function: tRNA dimethylallyltransferase activity.
GO:0008033; Biological process: tRNA processing.

Cross-references [?]

Pfam PF01715; IPPT; 1;
TIGRFAMs TIGR00174; MiaA; 1;

Features [?]

From: MIAA_ECOLI (P16384)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     17     24       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616        
BINDING (Optional)     19     24       /ligand="substrate     [ST]-x-x-[GN]-K-[ST]  
REGION (Optional)     42     45       Interaction with substrate tRNA     D-S-x-x  
REGION (Optional)     166     170       Interaction with substrate tRNA     Q-R-x-x-R  
REGION (Optional)     247     252       Interaction with substrate tRNA     R-C-V-G-Y-R  
REGION (Optional)     280     287       Interaction with substrate tRNA     K-R-Q-I-T-W-L-R  
SITE (Optional)     108     108       Interaction with substrate tRNA     [TS]  
SITE (Optional)     130     130       Interaction with substrate tRNA     R  

Additional information [?]

Size range 279-363 amino acids
Related rules None
Fusion None
Comments Possible wrong start in a number of cases


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