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HAMAP rule MF_00185

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General rule information [?]

Accession MF_00185
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 34)
Name IPP_trans
Scope(s) Bacteria
Template(s) P16384 (MIAA_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_00185 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier MIAA
Protein name RecName: Full=tRNA dimethylallyltransferase;
AltName: Full=Dimethylallyl diphosphate:tRNA dimethylallyltransferase;
                 Short=DMAPP:tRNA dimethylallyltransferase;
AltName: Full=Isopentenyl-diphosphate:tRNA isopentenyltransferase;
                 Short=IPP transferase;
Gene name Name=miaA;

Comments [?]

FUNCTIONCatalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A).
CATALYTIC ACTIVITY Reaction=adenosine(37) in tRNA + dimethylallyl diphosphate = diphosphate + N(6)-dimethylallyladenosine(37) in tRNA; Xref=Rhea:RHEA:26482, Rhea:RHEA-COMP:10162, Rhea:RHEA-COMP:10375, ChEBI:CHEBI:33019, ChEBI:CHEBI:57623, ChEBI:CHEBI:74411, ChEBI:CHEBI:74415; EC=;
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
SIMILARITYBelongs to the IPP transferase family.

Keywords [?]

Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
GO:0052381; Molecular function:tRNA dimethylallyltransferase activity
GO:0008033; Biological process:tRNA processing

Cross-references [?]

Pfam PF01715; IPPT; 1;
NCBIfam TIGR00174; MiaA; 1;

Features [?]

From: MIAA_ECOLI (P16384)
Key From To Description Tag Condition FTGroup
BINDING 17 24 /ligand="ATP"
BINDING 19 24 /ligand="substrate" [ST]-x-x-[GN]-K-[ST]
REGION 42 45 /note="Interaction with substrate tRNA" D-S-x-x
REGION 166 170 /note="Interaction with substrate tRNA" Q-R-x-x-R
REGION 247 252 /note="Interaction with substrate tRNA" R-C-V-G-Y-R
REGION 280 287 /note="Interaction with substrate tRNA" K-R-Q-I-T-W-L-R
SITE 108 108 /note="Interaction with substrate tRNA" [TS]
SITE 130 130 /note="Interaction with substrate tRNA" R

Additional information [?]

Size range 279-363 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments Possible wrong start in a number of cases

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