HAMAP rule MF_00193
General rule information
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| Accession | MF_00193 |
| Dates | 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 37) |
| Name | NadE_ammonia_dep |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P08164 (NADE_BACSU); P18843 (NADE_ECOLI); Q81RP3 (NADE_BACAN); [ Recover all ] |
| Triggered by |
HAMAP; MF_00193 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | NADE |
| Protein name | RecName: Full=NH(3)-dependent NAD(+) synthetase; EC=6.3.1.5; |
| Gene name | Name=nadE; |
Comments
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| FUNCTION | Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source. |
| CATALYTIC ACTIVITY | Reaction=ATP + deamido-NAD(+) + NH4(+) = AMP + diphosphate + H(+) + NAD(+); Xref=Rhea:RHEA:21188, ChEBI:CHEBI:15378, ChEBI:CHEBI:28938, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57540, ChEBI:CHEBI:58437, ChEBI:CHEBI:456215; EC=6.3.1.5; |
| PATHWAY | Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. |
| SUBUNIT | Homodimer. |
| SIMILARITY | Belongs to the NAD synthetase family. |
Keywords
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Gene Ontology
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| GO:0005524; Molecular function:ATP binding |
| GO:0008795; Molecular function:NAD+ synthase activity |
| GO:0009435; Biological process:NAD biosynthetic process |
Cross-references
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Features
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| From: NADE_BACSU (P08164) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 45 | 52 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[GA]-[ILVM]-S-G-G-x-D-S | ||||||||
| BINDING | 258 | 259 | /ligand="deamido-NAD(+)" /ligand_id="ChEBI:CHEBI:58437" /ligand_note="ligand shared between two neighboring subunits" /note="in other chain" |
H-[KR] | ||||||||
| BINDING | 51 | 51 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | ||||||||
| BINDING | 163 | 163 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
E | ||||||||
| BINDING | 138 | 138 | /ligand="deamido-NAD(+)" /ligand_id="ChEBI:CHEBI:58437" /ligand_note="ligand shared between two neighboring subunits" /note="in other chain" |
R | ||||||||
| BINDING | 158 | 158 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
T | ||||||||
| BINDING | 171 | 171 | /ligand="deamido-NAD(+)" /ligand_id="ChEBI:CHEBI:58437" /ligand_note="ligand shared between two neighboring subunits" /note="in other chain" |
K | ||||||||
| BINDING | 178 | 178 | /ligand="deamido-NAD(+)" /ligand_id="ChEBI:CHEBI:58437" /ligand_note="ligand shared between two neighboring subunits" |
D | ||||||||
| BINDING | 187 | 187 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
K | ||||||||
| BINDING | 209 | 209 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[ST] | ||||||||
Additional information
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| Size range | 230-350 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |