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Annotation rule MF_00193
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General rule information [?]

Accession MF_00193
Dates 1-JUN-2001 (Created)
18-NOV-2019 (Last updated, Version 33)
Name NadE_ammonia_dep
Scope
Bacteria
Archaea
Templates P08164 (NADE_BACSU); P18843 (NADE_ECOLI); Q81RP3 (NADE_BACAN): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
NADE
Protein name
RecName: Full=NH(3)-dependent NAD(+) synthetase;
EC 6.3.1.5;
Gene name
nadE

Comments [?]

Function Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source.
Catalytic activity RHEA:21188: ATP + deamido-NAD(+) + NH4(+) = AMP + diphosphate + H(+) + NAD(+)
EC 6.3.1.5
Pathway Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1.
Subunit Homodimer.
Similarity Belongs to the NAD synthetase family.

Keywords [?]

ATP-binding
Ligase
Magnesium
Metal-binding
NAD
Nucleotide-binding

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0008795; Molecular function: NAD+ synthase activity.
GO:0009435; Biological process: NAD biosynthetic process.

Cross-references [?]

Pfam PF02540; NAD_synthase; 1;
TIGRFAMs TIGR00552; nadE; 1;

Features [?]

From: NADE_BACSU (P08164)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     45     52       ATP     [GA]-[ILVM]-S-G-G-x-D-S  
NP_BIND (Optional)     258     259       Deamido-NAD     H-[KR]  
METAL     51     51       Magnesium     D  
METAL     163     163       Magnesium     E  
BINDING (Optional)     138     138       Deamido-NAD     R  
BINDING (Optional)     158     158       ATP     T  
BINDING (Optional)     171     171       Deamido-NAD     K  
BINDING (Optional)     178     178       Deamido-NAD     D  
BINDING (Optional)     187     187       ATP     K  
BINDING (Optional)     209     209       ATP; via amide nitrogen and carbonyl oxygen     [ST]  

Additional information [?]

Size range 230-350 amino acids
Related rules None
Fusion None


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