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HAMAP rule MF_00200

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General rule information [?]

Accession MF_00200
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 30)
Name RTC
Template P46849 (RTCA_ECOLI)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=RNA 3'-terminal phosphate cyclase;
Short=RNA cyclase;
Short=RNA-3'-phosphate cyclase;
Gene name

Comments [?]

Function Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.
Catalytic activity RHEA:23976: a 3'-end 3'-phospho-ribonucleotide-RNA + ATP = a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + AMP + diphosphate
Subcellular location Cytoplasm.
Similarity Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Keywords [?]

Gene Ontology [?]

GO:0003963; Molecular function: RNA-3'-phosphate cyclase activity.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE PS01287; RTC; 1;
Pfam PF01137; RTC; 1;
NCBIfam TIGR03399; RNA_3prim_cycl; 1;

Features [?]

From: RTCA_ECOLI (P46849)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     283     287       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     [YHF]-x-x-D-[QH]  
ACT_SITE     308     308       Tele-AMP-histidine intermediate     H  
BINDING     103     103       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     [QD]  

Additional information [?]

Size range 319-400 amino acids
Related rules None
Fusion None
Comments Generally, RNA 3'-terminal phosphate cyclases are ATP dependent, but P.furiosus enzyme preferentially uses GTP rather than ATP (PubMed:22074260).