HAMAP rule MF_00200

General rule information [?]

Accession	MF_00200
Dates	1-JUN-2001 (Created) 1-JUN-2023 (Last updated, Version 30)

Name

RTC

Scope

Bacteria

Archaea

Template

P46849 (RTCA_ECOLI)

Triggered by

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

RTCA

Protein name

RecName:		Full=RNA 3'-terminal phosphate cyclase;
		Short=RNA cyclase;
		Short=RNA-3'-phosphate cyclase;
		EC 6.5.1.4;

Gene name

rtcA

Comments [?]

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.
Catalytic activity	RHEA:23976: a 3'-end 3'-phospho-ribonucleotide-RNA + ATP = a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + AMP + diphosphate EC 6.5.1.4
Subcellular location	Cytoplasm.
Similarity	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Keywords [?]

Gene Ontology [?]

GO:0003963; Molecular function: RNA-3'-phosphate cyclase activity.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Features [?]

From: RTCA_ECOLI (P46849)

Key From To Description Tag Condition FTGroup

BINDING 283 287 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 [YHF]-x-x-D-[QH]

ACT_SITE 308 308 Tele-AMP-histidine intermediate H

BINDING 103 103 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 [QD]

Additional information [?]

Size range	319-400 amino acids
Related rules	None
Fusion	None
Comments	Generally, RNA 3'-terminal phosphate cyclases are ATP dependent, but P.furiosus enzyme preferentially uses GTP rather than ATP (PubMed:22074260).