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HAMAP rule MF_00200

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General rule information [?]

Accession MF_00200
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 30)
Name RTC
Scope(s) Bacteria
Template(s) P46849 (RTCA_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_00200 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier RTCA
Protein name RecName: Full=RNA 3'-terminal phosphate cyclase;
                 Short=RNA cyclase;
                 Short=RNA-3'-phosphate cyclase;
Gene name Name=rtcA;

Comments [?]

FUNCTIONCatalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.
CATALYTIC ACTIVITY Reaction=a 3'-end 3'-phospho-ribonucleotide-RNA + ATP = a 3'-end 2',3'- cyclophospho-ribonucleotide-RNA + AMP + diphosphate; Xref=Rhea:RHEA:23976, Rhea:RHEA-COMP:10463, Rhea:RHEA-COMP:10464, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:83062, ChEBI:CHEBI:83064, ChEBI:CHEBI:456215; EC=;
SIMILARITYBelongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Keywords [?]

Gene Ontology [?]

GO:0003963; Molecular function:RNA-3'-phosphate cyclase activity
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

PROSITE PS01287; RTC; 1;
Pfam PF01137; RTC; 1;
NCBIfam TIGR03399; RNA_3prim_cycl; 1;

Features [?]

From: RTCA_ECOLI (P46849)
Key From To Description Tag Condition FTGroup
BINDING 283 287 /ligand="ATP"
ACT_SITE 308 308 /note="Tele-AMP-histidine intermediate" H
BINDING 103 103 /ligand="ATP"

Additional information [?]

Size range 319-400 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments Generally, RNA 3'-terminal phosphate cyclases are ATP dependent, but P.furiosus enzyme preferentially uses GTP rather than ATP (PubMed:22074260).

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