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Annotation rule MF_00200 |
Accession | MF_00200 |
Dates | 1-JUN-2001 (Created) 18-NOV-2019 (Last updated, Version 25) |
Name | RTC |
Scope | Bacteria
Archaea |
Template | P46849 (RTCA_ECOLI) |
Triggered by |
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Protein name |
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Gene name |
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Function | Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing. |
Catalytic activity | RHEA:23976: (RNA)-3'-(3'-phospho-ribonucleoside) + ATP = (RNA)-3'-(2',3'-cyclophospho)-ribonucleoside + AMP + diphosphate
EC 6.5.1.4 |
Subcellular location | Cytoplasm. |
Similarity | Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily. |
From: RTCA_ECOLI (P46849) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
NP_BIND | 283 | 287 | ATP | [YHF]-x-x-D-[QH] | ||||||||
ACT_SITE | 308 | 308 | Tele-AMP-histidine intermediate | H | ||||||||
BINDING | 103 | 103 | ATP | [QD] |
Size range | 319-400 amino acids |
Related rules | None |
Fusion | None |
Comments | Generally, RNA 3'-terminal phosphate cyclases are ATP dependent, but P.furiosus enzyme preferentially uses GTP rather than ATP (PubMed:22074260). |