HAMAP rule MF_00204
General rule information
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| Accession | MF_00204 |
| Dates | 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 37) |
| Name | UvrB |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P56981 (UVRB_BACCA); P0A8F8 (UVRB_ECOLI); Q56243 (UVRB_THET8); [ Recover all ] |
| Triggered by |
HAMAP; MF_00204 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | UVRB |
| Protein name | RecName: Full=UvrABC system protein B; Short=Protein UvrB; AltName: Full=Excinuclease ABC subunit B; |
| Gene name | Name=uvrB; |
Comments
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| FUNCTION | The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage. |
| SUBUNIT | Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| DOMAIN | The beta-hairpin motif is involved in DNA binding. |
| SIMILARITY | Belongs to the UvrB family. |
Keywords
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| Cytoplasm |
| DNA damage |
| SOS response |
| Excision nuclease |
| DNA repair |
| DNA excision |
| ATP-binding |
| Nucleotide-binding |
Gene Ontology
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| GO:0005524; Molecular function:ATP binding |
| GO:0003677; Molecular function:DNA binding |
| GO:0009381; Molecular function:excinuclease ABC activity |
| GO:0006281; Biological process:DNA repair |
| GO:0006289; Biological process:nucleotide-excision repair |
| GO:0009432; Biological process:SOS response |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| Pfam | PF00271; Helicase_C; 1; |
| Pfam | PF04851; ResIII; 1; |
| Pfam | PF02151; UVR; 1; |
| NCBIfam | TIGR00631; Uvrb; 1; |
| PROSITE | PS51192; HELICASE_ATP_BIND_1; 1; |
| PROSITE | PS51194; HELICASE_CTER; 1; |
| PROSITE | PS50151; UVR; 0-1; |
Features
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| From: UVRB_ECOLI (P0A8F8) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 39 | 46 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G-[IVAT]-T-G-[ST]-G-K-[ST] | ||||||||
| MOTIF | 92 | 115 | /note="Beta-hairpin" | |||||||||
Additional information
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| Size range | 645-709 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |