HAMAP rule MF_00204

General rule information [?]

Accession	MF_00204
Dates	1-JUN-2001 (Created) 1-JUN-2023 (Last updated, Version 37)

Name

UvrB

Scope

Bacteria

Archaea

Templates

P56981 (UVRB_BACCA); P0A8F8 (UVRB_ECOLI); Q56243 (UVRB_THET8): [Recover all]

Triggered by

HAMAP; MF_00204 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

UVRB

Protein name

RecName:		Full=UvrABC system protein B;
		Short=Protein UvrB;
AltName:		Full=Excinuclease ABC subunit B;

Gene name

uvrB

Comments [?]

Function	The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.
Subunit	Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.
Subcellular location	Cytoplasm.
Domain	The beta-hairpin motif is involved in DNA binding.
Similarity	Belongs to the UvrB family.

Keywords [?]

Cytoplasm
DNA damage
SOS response
Excision nuclease
DNA repair
DNA excision
ATP-binding
Nucleotide-binding

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0003677; Molecular function: DNA binding.
GO:0009381; Molecular function: excinuclease ABC activity.
GO:0006281; Biological process: DNA repair.
GO:0006289; Biological process: nucleotide-excision repair.
GO:0009432; Biological process: SOS response.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam	PF00271; Helicase_C; 1;
	PF04851; ResIII; 1;
	PF02151; UVR; 1;
NCBIfam	TIGR00631; Uvrb; 1;
PROSITE	PS51192; HELICASE_ATP_BIND_1; 1; trigger=PRU00541;
	PS51194; HELICASE_CTER; 1; trigger=PRU00542;
	PS50151; UVR; 0-1; trigger=PRU00217;

Features [?]

From: UVRB_ECOLI (P0A8F8)

Key From To Description Tag Condition FTGroup

BINDING 39 46 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 G-[IVAT]-T-G-[ST]-G-K-[ST]

MOTIF 92 115 Beta-hairpin

Additional information [?]

Size range	645-709 amino acids
Related rules	None
Fusion	None

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