HAMAP logo

HAMAP rule MF_00204

Send feedback

General rule information [?]

Accession MF_00204
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 37)
Name UvrB
Templates P56981 (UVRB_BACCA); P0A8F8 (UVRB_ECOLI); Q56243 (UVRB_THET8): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=UvrABC system protein B;
Short=Protein UvrB;
AltName: Full=Excinuclease ABC subunit B;
Gene name

Comments [?]

Function The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.
Subunit Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.
Subcellular location Cytoplasm.
Domain The beta-hairpin motif is involved in DNA binding.
Similarity Belongs to the UvrB family.

Keywords [?]

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0003677; Molecular function: DNA binding.
GO:0009381; Molecular function: excinuclease ABC activity.
GO:0006281; Biological process: DNA repair.
GO:0006289; Biological process: nucleotide-excision repair.
GO:0009432; Biological process: SOS response.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00271; Helicase_C; 1;
PF04851; ResIII; 1;
PF02151; UVR; 1;
NCBIfam TIGR00631; Uvrb; 1;
PROSITE PS51192; HELICASE_ATP_BIND_1; 1; trigger=PRU00541;
PS51194; HELICASE_CTER; 1; trigger=PRU00542;
PS50151; UVR; 0-1; trigger=PRU00217;

Features [?]

Key     From     To       Description   Tag   Condition   FTGroup
BINDING     39     46       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     G-[IVAT]-T-G-[ST]-G-K-[ST]  
MOTIF     92     115       Beta-hairpin        

Additional information [?]

Size range 645-709 amino acids
Related rules None
Fusion None