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HAMAP rule MF_00210

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General rule information [?]

PURL https://purl.expasy.org/hamap/rule/MF_00210
Accession MF_00210
Dates 28-FEB-2005 (Created)
12-MAR-2024 (Last updated, Version 33)
Name EPSP_synth
Scope(s) Bacteria
Archaea
Template(s) P0A6D3; P9WPY5; Q9R4E4; Q83E11; Q9S400; Q9KRB0; [ Recover all ]
Triggered by HAMAP; MF_00210 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier AROA
Protein name RecName: Full=3-phosphoshikimate 1-carboxyvinyltransferase;
                 EC=2.5.1.19;
AltName: Full=5-enolpyruvylshikimate-3-phosphate synthase;
                 Short=EPSP synthase;
                 Short=EPSPS;
Gene name Name=aroA;

Comments [?]

FUNCTIONCatalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.
CATALYTIC ACTIVITY Reaction=3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1- carboxyvinyl)-3-phosphoshikimate + phosphate; Xref=Rhea:RHEA:21256, ChEBI:CHEBI:43474, ChEBI:CHEBI:57701, ChEBI:CHEBI:58702, ChEBI:CHEBI:145989; EC=2.5.1.19; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:21257;
case <OC:Bacteria>
PATHWAYMetabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7.
end case
case <OC:Archaea>
PATHWAYMetabolic intermediate biosynthesis; chorismate biosynthesis.
end case
SUBUNITMonomer.
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYBelongs to the EPSP synthase family.

Keywords [?]

Amino-acid biosynthesis
Aromatic amino acid biosynthesis
Cytoplasm
Transferase

Gene Ontology [?]

GO:0003866; Molecular function:3-phosphoshikimate 1-carboxyvinyltransferase activity
GO:0009423; Biological process:chorismate biosynthetic process
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF00275; EPSP_synthase; 1;
PIRSF PIRSF000505; EPSPS; 1;
NCBIfam TIGR01356; AroA; 1;
PROSITE PS00104; EPSP_SYNTHASE_1; 1;
PROSITE PS00885; EPSP_SYNTHASE_2; 1;

Features [?]

From: AROA_ECOLI (P0A6D3)
Key From To Description Tag Condition FTGroup
ACT_SITE 313 313 /note="Proton acceptor" [DE]
BINDING 22 22 /ligand="3-phosphoshikimate"
/ligand_id="ChEBI:CHEBI:145989"		 K
BINDING 22 22 /ligand="phosphoenolpyruvate"
/ligand_id="ChEBI:CHEBI:58702"		 K
BINDING 23 23 /ligand="3-phosphoshikimate"
/ligand_id="ChEBI:CHEBI:145989"		 S
BINDING 27 27 /ligand="3-phosphoshikimate"
/ligand_id="ChEBI:CHEBI:145989"		 R
BINDING 96 96 /ligand="phosphoenolpyruvate"
/ligand_id="ChEBI:CHEBI:58702"		 G
BINDING 124 124 /ligand="phosphoenolpyruvate"
/ligand_id="ChEBI:CHEBI:58702"		 R
BINDING 169 169 /ligand="3-phosphoshikimate"
/ligand_id="ChEBI:CHEBI:145989"		 [ST]
BINDING 170 170 /ligand="3-phosphoshikimate"
/ligand_id="ChEBI:CHEBI:145989"		 [ST]
BINDING 171 171 /ligand="3-phosphoshikimate"
/ligand_id="ChEBI:CHEBI:145989"		 [QN]
BINDING 171 171 /ligand="phosphoenolpyruvate"
/ligand_id="ChEBI:CHEBI:58702"		 [QN]
BINDING 197 197 /ligand="3-phosphoshikimate"
/ligand_id="ChEBI:CHEBI:145989"		 [ST]
BINDING 313 313 /ligand="3-phosphoshikimate"
/ligand_id="ChEBI:CHEBI:145989"		 [DE]
BINDING 336 336 /ligand="3-phosphoshikimate"
/ligand_id="ChEBI:CHEBI:145989"		 [NQ]
BINDING 340 340 /ligand="3-phosphoshikimate"
/ligand_id="ChEBI:CHEBI:145989"		 [KHQ]
BINDING 344 344 /ligand="phosphoenolpyruvate"
/ligand_id="ChEBI:CHEBI:58702"		 R
BINDING 386 386 /ligand="phosphoenolpyruvate"
/ligand_id="ChEBI:CHEBI:58702"		 R
BINDING 411 411 /ligand="phosphoenolpyruvate"
/ligand_id="ChEBI:CHEBI:58702"		 [KQ]

Additional information [?]

Size range 399-469 amino acids
Related rules MF_03143
Fusion Nter: None Cter: None
Comments Highly divergent AERSA; sequence not included in alignment. Divergent duplicate aroA sequence in AERPE: APE1404. Possible wrong start in SYNY3, THEMA.



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