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Annotation rule MF_00210
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General rule information [?]

Accession MF_00210
Dates 1-JUN-2001 (Created)
18-NOV-2019 (Last updated, Version 44)
Name EPSP_synth
Scope
Bacteria
Archaea
Templates P0A6D3 (AROA_ECOLI); P9WPY5 (AROA_MYCTU); Q9R4E4 (AROA_AGRSC); Q83E11 (AROA_COXBU); Q9S400 (AROA_STRPN); Q9KRB0 (AROA_VIBCH): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
AROA
Protein name
RecName: Full=3-phosphoshikimate 1-carboxyvinyltransferase;
EC 2.5.1.19;
AltName: Full=5-enolpyruvylshikimate-3-phosphate synthase;
Short=EPSP synthase;
Short=EPSPS;
Gene name
aroA

Comments [?]

Function Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.
Catalytic activity RHEA:21256: 3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate
EC 2.5.1.19
case <OC:Bacteria>
Pathway Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7.
end case
case <OC:Archaea>
Pathway Metabolic intermediate biosynthesis; chorismate biosynthesis.
end case
Subunit Monomer.
Subcellular location Cytoplasm.
Similarity Belongs to the EPSP synthase family.

Keywords [?]


Gene Ontology [?]

GO:0003866; Molecular function: 3-phosphoshikimate 1-carboxyvinyltransferase activity.
GO:0009073; Biological process: aromatic amino acid family biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00275; EPSP_synthase; 1;
PIRSF PIRSF000505; EPSPS; 1;
TIGRFAMs TIGR01356; AroA; 1;
PROSITE PS00104; EPSP_SYNTHASE_1; 1;
PS00885; EPSP_SYNTHASE_2; 1;

Features [?]

From: AROA_ECOLI (P0A6D3)
Key     From     To       Description   Tag   Condition   FTGroup
REGION (Optional)     22     23       Shikimate-3-phosphate binding     K-S  
REGION (Optional)     94     97       Phosphoenolpyruvate     [LNAGDEQS]-x-[GA]-[TSIAV]  
REGION (Optional)     169     171       Shikimate-3-phosphate binding     [ST]-[ST]-[QN]  
ACT_SITE     313     313       Proton acceptor     [DE]  
ACT_SITE     341     341       Proton donor     E  
BINDING     27     27       Shikimate-3-phosphate     R  
BINDING     124     124       Phosphoenolpyruvate     R  
BINDING     197     197       Shikimate-3-phosphate     [ST]  
BINDING     336     336       Shikimate-3-phosphate     [NQ]  
BINDING     340     340       Shikimate-3-phosphate     [KQ]  
BINDING     344     344       Phosphoenolpyruvate     R  
BINDING     386     386       Phosphoenolpyruvate     R  
BINDING     411     411       Phosphoenolpyruvate     [KQ]  

Additional information [?]

Size range 399-469 amino acids
Related rules MF_03143 (ARO1 supersedes the current rule)
Fusion None
Comments Highly divergent AERSA; sequence not included in alignment. Divergent duplicate aroA sequence in AERPE: APE1404. Possible wrong start in SYNY3, THEMA.