HAMAP rule MF_00210
General rule information
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Accession | MF_00210 |
Dates | 1-JUN-2001 (Created)
12-MAR-2024 (Last updated, Version 50) |
Name | EPSP_synth |
Scope(s) |
Bacteria Archaea |
Template(s) | P0A6D3 (AROA_ECOLI); P9WPY5 (AROA_MYCTU); Q9R4E4 (AROA_AGRSC); Q83E11 (AROA_COXBU); Q9S400 (AROA_STRPN); Q9KRB0 (AROA_VIBCH); [ Recover all ] |
Triggered by |
HAMAP; MF_00210 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | AROA |
Protein name | RecName: Full=3-phosphoshikimate 1-carboxyvinyltransferase; EC=2.5.1.19; AltName: Full=5-enolpyruvylshikimate-3-phosphate synthase; Short=EPSP synthase; Short=EPSPS; |
Gene name | Name=aroA; |
Comments
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FUNCTION | Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate. |
CATALYTIC ACTIVITY | Reaction=3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1- carboxyvinyl)-3-phosphoshikimate + phosphate; Xref=Rhea:RHEA:21256, ChEBI:CHEBI:43474, ChEBI:CHEBI:57701, ChEBI:CHEBI:58702, ChEBI:CHEBI:145989; EC=2.5.1.19; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:21257; |
case <OC:Bacteria> | |
PATHWAY | Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. |
end case | |
case <OC:Archaea> | |
PATHWAY | Metabolic intermediate biosynthesis; chorismate biosynthesis. |
end case | |
SUBUNIT | Monomer. |
SUBCELLULAR LOCATION | Cytoplasm. |
SIMILARITY | Belongs to the EPSP synthase family. |
Keywords
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Gene Ontology
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GO:0003866; Molecular function:3-phosphoshikimate 1-carboxyvinyltransferase activity |
GO:0009423; Biological process:chorismate biosynthetic process |
GO:0005737; Cellular component:cytoplasm |
Cross-references
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Pfam | PF00275; EPSP_synthase; 1; |
PIRSF | PIRSF000505; EPSPS; 1; |
NCBIfam | TIGR01356; AroA; 1; |
PROSITE | PS00104; EPSP_SYNTHASE_1; 1; |
PROSITE | PS00885; EPSP_SYNTHASE_2; 1; |
Features
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From: AROA_ECOLI (P0A6D3) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
ACT_SITE | 313 | 313 | /note="Proton acceptor" | [DE] | ||||||||
BINDING | 22 | 22 | /ligand="3-phosphoshikimate" /ligand_id="ChEBI:CHEBI:145989" |
K | ||||||||
BINDING | 22 | 22 | /ligand="phosphoenolpyruvate" /ligand_id="ChEBI:CHEBI:58702" |
K | ||||||||
BINDING | 23 | 23 | /ligand="3-phosphoshikimate" /ligand_id="ChEBI:CHEBI:145989" |
S | ||||||||
BINDING | 27 | 27 | /ligand="3-phosphoshikimate" /ligand_id="ChEBI:CHEBI:145989" |
R | ||||||||
BINDING | 96 | 96 | /ligand="phosphoenolpyruvate" /ligand_id="ChEBI:CHEBI:58702" |
G | ||||||||
BINDING | 124 | 124 | /ligand="phosphoenolpyruvate" /ligand_id="ChEBI:CHEBI:58702" |
R | ||||||||
BINDING | 169 | 169 | /ligand="3-phosphoshikimate" /ligand_id="ChEBI:CHEBI:145989" |
[ST] | ||||||||
BINDING | 170 | 170 | /ligand="3-phosphoshikimate" /ligand_id="ChEBI:CHEBI:145989" |
[ST] | ||||||||
BINDING | 171 | 171 | /ligand="3-phosphoshikimate" /ligand_id="ChEBI:CHEBI:145989" |
[QN] | ||||||||
BINDING | 171 | 171 | /ligand="phosphoenolpyruvate" /ligand_id="ChEBI:CHEBI:58702" |
[QN] | ||||||||
BINDING | 197 | 197 | /ligand="3-phosphoshikimate" /ligand_id="ChEBI:CHEBI:145989" |
[ST] | ||||||||
BINDING | 313 | 313 | /ligand="3-phosphoshikimate" /ligand_id="ChEBI:CHEBI:145989" |
[DE] | ||||||||
BINDING | 336 | 336 | /ligand="3-phosphoshikimate" /ligand_id="ChEBI:CHEBI:145989" |
[NQ] | ||||||||
BINDING | 340 | 340 | /ligand="3-phosphoshikimate" /ligand_id="ChEBI:CHEBI:145989" |
[KHQ] | ||||||||
BINDING | 344 | 344 | /ligand="phosphoenolpyruvate" /ligand_id="ChEBI:CHEBI:58702" |
R | ||||||||
BINDING | 386 | 386 | /ligand="phosphoenolpyruvate" /ligand_id="ChEBI:CHEBI:58702" |
R | ||||||||
BINDING | 411 | 411 | /ligand="phosphoenolpyruvate" /ligand_id="ChEBI:CHEBI:58702" |
[KQ] |
Additional information
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Size range | 399-469 amino acids |
Related rules |
MF_03143 |
Fusion | Nter: None Cter: None |
Comments | Highly divergent AERSA; sequence not included in alignment. Divergent duplicate aroA sequence in AERPE: APE1404. Possible wrong start in SYNY3, THEMA. |