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HAMAP rule MF_00219

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General rule information [?]

Accession MF_00219
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 40)
Name PyrC_classII
Scope
Bacteria
Templates P05020 (PYRC_ECOLI); A6T7D6 (PYRC_KLEP7); B1IV40 (PYRC_ECOLC); P06204 (PYRC_SALTY); Q8ZFU4 (PYRC_YERPE): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
PYRC
Protein name
RecName: Full=Dihydroorotase;
Short=DHOase;
EC 3.5.2.3;
Gene name
pyrC

Comments [?]

Function Catalyzes the reversible cyclization of carbamoyl aspartate to dihydroorotate.
Catalytic activity RHEA:24296: (S)-dihydroorotate + H2O = H(+) + N-carbamoyl-L-aspartate
EC 3.5.2.3
Cofactor Zn(2+)
Note: Binds 2 Zn(2+) ions per subunit.
Pathway Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3.
Subunit Homodimer.
Similarity Belongs to the metallo-dependent hydrolases superfamily. DHOase family. Class II DHOase subfamily.

Keywords [?]


Gene Ontology [?]

GO:0004151; Molecular function: dihydroorotase activity.
GO:0008270; Molecular function: zinc ion binding.
GO:0006221; Biological process: pyrimidine nucleotide biosynthetic process.

Cross-references [?]

Pfam PF01979; Amidohydro_1; 1;
PIRSF PIRSF001237; DHOdimr; 1;
NCBIfam TIGR00856; PyrC_dimer; 1;
PROSITE PS00482; DIHYDROOROTASE_1; 1;
PS00483; DIHYDROOROTASE_2; 1;

Features [?]

From: PYRC_ECOLI (P05020)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING (Optional)     19     21       /ligand="substrate     H-[LFV]-R  
ACT_SITE     251     251             D  
BINDING     17     17       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1     H  
BINDING     19     19       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1     H  
BINDING     103     103       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1" /note="via carbamate group     K  
BINDING     103     103       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2" /note="via carbamate group     K  
BINDING     140     140       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2     H  
BINDING     178     178       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2     H  
BINDING     251     251       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1     D  
BINDING (Optional)     45     45       /ligand="substrate     N  
BINDING (Optional)     140     140       /ligand="substrate     H  
BINDING (Optional)     223     223       /ligand="substrate     L  
BINDING (Optional)     255     255       /ligand="substrate     H  
BINDING (Optional)     267     267       /ligand="substrate     A  
MOD_RES     103     103       N6-carboxylysine     K  

Additional information [?]

Size range 300-400 amino acids
Related rules MF_00220 (PYRC)
Fusion None
Comments Classification into subfamilies was done according to PubMed:24332717