HAMAP rule MF_00219

General rule information [?]

Accession	MF_00219
Dates	1-JUN-2001 (Created) 1-JUN-2023 (Last updated, Version 40)

Name	PyrC_classII

Scope

Bacteria

Templates

P05020 (PYRC_ECOLI); A6T7D6 (PYRC_KLEP7); B1IV40 (PYRC_ECOLC); P06204 (PYRC_SALTY); Q8ZFU4 (PYRC_YERPE): [Recover all]

Triggered by

HAMAP; MF_00219 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

PYRC

Protein name

RecName:		Full=Dihydroorotase;
		Short=DHOase;
		EC 3.5.2.3;

Gene name

pyrC

Comments [?]

Function	Catalyzes the reversible cyclization of carbamoyl aspartate to dihydroorotate.
Catalytic activity	RHEA:24296: (S)-dihydroorotate + H2O = H(+) + N-carbamoyl-L-aspartate EC 3.5.2.3
Cofactor	Zn(2+) Note: Binds 2 Zn(2+) ions per subunit.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3.
Subunit	Homodimer.
Similarity	Belongs to the metallo-dependent hydrolases superfamily. DHOase family. Class II DHOase subfamily.

Keywords [?]

Hydrolase
Metal-binding
Pyrimidine biosynthesis
Zinc

Gene Ontology [?]

GO:0004151; Molecular function: dihydroorotase activity.
GO:0008270; Molecular function: zinc ion binding.
GO:0006221; Biological process: pyrimidine nucleotide biosynthetic process.

Cross-references [?]

Pfam	PF01979; Amidohydro_1; 1;
PIRSF	PIRSF001237; DHOdimr; 1;
NCBIfam	TIGR00856; PyrC_dimer; 1;
PROSITE	PS00482; DIHYDROOROTASE_1; 1;
	PS00483; DIHYDROOROTASE_2; 1;

Features [?]

From: PYRC_ECOLI (P05020)

Key From To Description Tag Condition FTGroup

BINDING (Optional) 19 21 /ligand="substrate H-[LFV]-R

ACT_SITE 251 251 D

BINDING 17 17 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1 H

BINDING 19 19 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1 H

BINDING 103 103 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1" /note="via carbamate group K

BINDING 103 103 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2" /note="via carbamate group K

BINDING 140 140 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2 H

BINDING 178 178 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2 H

BINDING 251 251 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1 D

BINDING (Optional) 45 45 /ligand="substrate N

BINDING (Optional) 140 140 /ligand="substrate H

BINDING (Optional) 223 223 /ligand="substrate L

BINDING (Optional) 255 255 /ligand="substrate H

BINDING (Optional) 267 267 /ligand="substrate A

MOD_RES 103 103 N6-carboxylysine K

Additional information [?]

Size range	300-400 amino acids
Related rules	MF_00220 (PYRC)
Fusion	None
Comments	Classification into subfamilies was done according to PubMed:24332717

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