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HAMAP rule MF_00225

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General rule information [?]

Accession MF_00225
Dates 1-JUN-2001 (Created)
14-MAY-2024 (Last updated, Version 44)
Name DHO_dh_type2
Scope(s) Archaea
Halobacteriales
Bacteria
Template(s) P0A7E1 (PYRD_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_00225 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier PYRD
Protein name RecName: Full=Dihydroorotate dehydrogenase (quinone);
                 EC=1.3.5.2;
AltName: Full=DHOdehase;
                 Short=DHOD;
                 Short=DHODase;
AltName: Full=Dihydroorotate oxidase;
Gene name Name=pyrD;

Comments [?]

FUNCTIONCatalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.
CATALYTIC ACTIVITY Reaction=(S)-dihydroorotate + a quinone = a quinol + orotate; Xref=Rhea:RHEA:30187, ChEBI:CHEBI:24646, ChEBI:CHEBI:30839, ChEBI:CHEBI:30864, ChEBI:CHEBI:132124; EC=1.3.5.2;
COFACTOR Name=FMN; Xref=ChEBI:CHEBI:58210; Note=Binds 1 FMN per subunit.;
PATHWAYPyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.
SUBUNITMonomer.
SUBCELLULAR LOCATIONCell membrane; Peripheral membrane protein.
SIMILARITYBelongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Keywords [?]


Gene Ontology [?]

GO:0004152; Molecular function:dihydroorotate dehydrogenase activity
GO:0044205; Biological process:'de novo' UMP biosynthetic process
GO:0005886; Cellular component:plasma membrane

Cross-references [?]

Pfam PF01180; DHO_dh; 1;
PIRSF PIRSF000164; DHO_oxidase; 1;
NCBIfam TIGR01036; PyrD_sub2; 1;
PROSITE PS00911; DHODEHASE_1; 1;
PROSITE PS00912; DHODEHASE_2; 1;

Features [?]

From: PYRD_ECOLI (P0A7E1)
Key From To Description Tag Condition FTGroup
BINDING 62 66 /ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"
[AP]-x-x-x-K
BINDING 318 319 /ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"
[YF]-[ST]
BINDING 111 115 /ligand="substrate" N-x-[MYLFN]-G-[FL]
BINDING 246 247 /ligand="substrate" N-T
ACT_SITE 175 175 /note="Nucleophile" [SC]
BINDING 66 66 /ligand="substrate" K
BINDING 86 86 /ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"
[TASG]
BINDING 139 139 /ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"
[NS]
BINDING 172 172 /ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"
N
BINDING 172 172 /ligand="substrate" N
BINDING 177 177 /ligand="substrate" N
BINDING 217 217 /ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"
K
BINDING 245 245 /ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"
[TASGV]
BINDING 268 268 /ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"
G
BINDING 297 297 /ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"
G

Additional information [?]

Size range 333-396 amino acids
Related rules MF_00224
Fusion Nter: None Cter: None



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