HAMAP rule MF_00225

General rule information [?]

Accession	MF_00225
Dates	1-JUN-2001 (Created) 1-JUN-2023 (Last updated, Version 43)

Name	DHO_dh_type2

Scope

Archaea; Halobacteriales

Bacteria

Template

P0A7E1 (PYRD_ECOLI)

Triggered by

HAMAP; MF_00225 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

PYRD

Protein name

RecName:		Full=Dihydroorotate dehydrogenase (quinone);
		EC 1.3.5.2;
AltName:		Full=DHOdehase;
		Short=DHOD;
		Short=DHODase;
AltName:		Full=Dihydroorotate oxidase;

Gene name

pyrD

Comments [?]

Function	Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.
Catalytic activity	RHEA:30187: (S)-dihydroorotate + a quinone = a quinol + orotate EC 1.3.5.2
Cofactor	FMN Note: Binds 1 FMN per subunit.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.
Subunit	Monomer.
Subcellular location	Cell membrane; Peripheral membrane protein.
Similarity	Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Keywords [?]

Cell membrane
Flavoprotein
FMN
Membrane
Oxidoreductase
Pyrimidine biosynthesis

Gene Ontology [?]

GO:0004152; Molecular function: dihydroorotate dehydrogenase activity.
GO:0006221; Biological process: pyrimidine nucleotide biosynthetic process.
GO:0005886; Cellular component: plasma membrane.

Cross-references [?]

Pfam	PF01180; DHO_dh; 1;
PIRSF	PIRSF000164; DHO_oxidase; 1;
NCBIfam	TIGR01036; PyrD_sub2; 1;
PROSITE	PS00911; DHODEHASE_1; 1;
	PS00912; DHODEHASE_2; 1;

Features [?]

From: PYRD_ECOLI (P0A7E1)

Key From To Description Tag Condition FTGroup

BINDING 62 66 /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 [AP]-x-x-x-K

BINDING 318 319 /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 [YF]-[ST]

BINDING 111 115 /ligand="substrate N-x-[MYLFN]-G-[FL]

BINDING 246 247 /ligand="substrate N-T

ACT_SITE 175 175 Nucleophile [SC]

BINDING 66 66 /ligand="substrate K

BINDING 86 86 /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 [TASG]

BINDING 139 139 /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 [NS]

BINDING 172 172 /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 N

BINDING 172 172 /ligand="substrate N

BINDING 177 177 /ligand="substrate N

BINDING 217 217 /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 K

BINDING 245 245 /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 [TASGV]

BINDING 268 268 /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 G

BINDING 297 297 /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 G

Additional information [?]

Size range	333-396 amino acids
Related rules	MF_00224 (PYRD)
Fusion	None

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