HAMAP logo

HAMAP rule MF_00225

Send feedback

General rule information [?]

Accession MF_00225
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 43)
Name DHO_dh_type2
Scope
Archaea; Halobacteriales
Bacteria
Template P0A7E1 (PYRD_ECOLI)

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
PYRD
Protein name
RecName: Full=Dihydroorotate dehydrogenase (quinone);
EC 1.3.5.2;
AltName: Full=DHOdehase;
Short=DHOD;
Short=DHODase;
AltName: Full=Dihydroorotate oxidase;
Gene name
pyrD

Comments [?]

Function Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.
Catalytic activity RHEA:30187: (S)-dihydroorotate + a quinone = a quinol + orotate
EC 1.3.5.2
Cofactor FMN
Note: Binds 1 FMN per subunit.
Pathway Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.
Subunit Monomer.
Subcellular location Cell membrane; Peripheral membrane protein.
Similarity Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Keywords [?]


Gene Ontology [?]

GO:0004152; Molecular function: dihydroorotate dehydrogenase activity.
GO:0006221; Biological process: pyrimidine nucleotide biosynthetic process.
GO:0005886; Cellular component: plasma membrane.

Cross-references [?]

Pfam PF01180; DHO_dh; 1;
PIRSF PIRSF000164; DHO_oxidase; 1;
NCBIfam TIGR01036; PyrD_sub2; 1;
PROSITE PS00911; DHODEHASE_1; 1;
PS00912; DHODEHASE_2; 1;

Features [?]

From: PYRD_ECOLI (P0A7E1)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     62     66       /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210     [AP]-x-x-x-K  
BINDING     318     319       /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210     [YF]-[ST]  
BINDING     111     115       /ligand="substrate     N-x-[MYLFN]-G-[FL]  
BINDING     246     247       /ligand="substrate     N-T  
ACT_SITE     175     175       Nucleophile     [SC]  
BINDING     66     66       /ligand="substrate     K  
BINDING     86     86       /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210     [TASG]  
BINDING     139     139       /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210     [NS]  
BINDING     172     172       /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210     N  
BINDING     172     172       /ligand="substrate     N  
BINDING     177     177       /ligand="substrate     N  
BINDING     217     217       /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210     K  
BINDING     245     245       /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210     [TASGV]  
BINDING     268     268       /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210     G  
BINDING     297     297       /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210     G  

Additional information [?]

Size range 333-396 amino acids
Related rules MF_00224 (PYRD)
Fusion None