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Annotation rule MF_00252
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General rule information [?]

Accession MF_00252
Dates 1-JUN-2001 (Created)
18-NOV-2019 (Last updated, Version 33)
Name Lys_tRNA_synth_class2
Scope
Bacteria
Archaea
Templates P0A8N3 (SYK1_ECOLI); P0A8N5 (SYK2_ECOLI): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
SYK
Protein name
RecName: Full=Lysine--tRNA ligase;
EC 6.1.1.6;
AltName: Full=Lysyl-tRNA synthetase;
Short=LysRS;
Gene name
lysS

Comments [?]

Catalytic activity RHEA:20792: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)
EC 6.1.1.6
Cofactor Mg(2+)
Note: Binds 3 Mg(2+) ions per subunit.
Subunit Homodimer.
Subcellular location Cytoplasm.
Similarity Belongs to the class-II aminoacyl-tRNA synthetase family.

Keywords [?]

case <FT:4> or <FT:5>
end case

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0004824; Molecular function: lysine-tRNA ligase activity.
GO:0000287; Molecular function: magnesium ion binding.
GO:0006430; Biological process: lysyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00152; tRNA-synt_2; 1;
PRINTS PR00982; TRNASYNTHLYS; 1;
TIGRFAMs TIGR00499; LysS_bact; 1;
PROSITE PS50862; AA_TRNA_LIGASE_II; 1;

Features [?]

From: SYK2_ECOLI (P0A8N5)
Key     From     To       Description   Tag   Condition   FTGroup
METAL     415     415       Magnesium 1     [ED]  
METAL     422     422       Magnesium 1     E  
METAL     422     422       Magnesium 2     E  
case <OC:Escherichia> or <OC:Shigella>
MOD_RES     114     114       N6-acetyllysine     K  
MOD_RES     156     156       N6-acetyllysine     K  
end case

Additional information [?]

Size range 485-597 amino acids
Related rules None
Fusion Nter: <Unknown>; Cter: None
Comments Lysyl-tRNA synthesis is catalyzed by two unrelated families of tRNA synthetase; class-I or class-II. Usually only one or the other is present, however Methanosarcina seem to have both. Weird insertion in AQUAE. Unknown C-terminal domain in SYK2_MYCTU.