HAMAP rule MF_00252
General rule information
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| Accession | MF_00252 |
| Dates | 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 36) |
| Name | Lys_tRNA_synth_class2 |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P0A8N3 (SYK1_ECOLI); P0A8N5 (SYK2_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_00252 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | SYK |
| Protein name | RecName: Full=Lysine--tRNA ligase; EC=6.1.1.6; AltName: Full=Lysyl-tRNA synthetase; Short=LysRS; |
| Gene name | Name=lysS; |
Comments
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| CATALYTIC ACTIVITY | Reaction=ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl- tRNA(Lys); Xref=Rhea:RHEA:20792, Rhea:RHEA-COMP:9696, Rhea:RHEA- COMP:9697, ChEBI:CHEBI:30616, ChEBI:CHEBI:32551, ChEBI:CHEBI:33019, ChEBI:CHEBI:78442, ChEBI:CHEBI:78529, ChEBI:CHEBI:456215; EC=6.1.1.6; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 3 Mg(2+) ions per subunit.; |
| SUBUNIT | Homodimer. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the class-II aminoacyl-tRNA synthetase family. |
Keywords
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| case <FT:4> or <FT:5> | |
| Acetylation | |
| end case | |
| Aminoacyl-tRNA synthetase | |
| ATP-binding | |
| Cytoplasm | |
| Ligase | |
| Magnesium | |
| Metal-binding | |
| Nucleotide-binding | |
| Protein biosynthesis | |
Gene Ontology
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| GO:0005524; Molecular function:ATP binding |
| GO:0004824; Molecular function:lysine-tRNA ligase activity |
| GO:0000287; Molecular function:magnesium ion binding |
| GO:0006430; Biological process:lysyl-tRNA aminoacylation |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| Pfam | PF00152; tRNA-synt_2; 1; |
| PRINTS | PR00982; TRNASYNTHLYS; 1; |
| NCBIfam | TIGR00499; LysS_bact; 1; |
| PROSITE | PS50862; AA_TRNA_LIGASE_II; 1; |
Features
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| From: SYK2_ECOLI (P0A8N5) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 415 | 415 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" |
[ED] | ||||||||
| BINDING | 422 | 422 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" |
E | ||||||||
| BINDING | 422 | 422 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" |
E | ||||||||
| case <OC:Escherichia> or <OC:Shigella> | ||||||||||||
| MOD_RES | 114 | 114 | /note="N6-acetyllysine" | K | ||||||||
| MOD_RES | 156 | 156 | /note="N6-acetyllysine" | K | ||||||||
| end case | ||||||||||||
Additional information
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| Size range | 485-597 amino acids |
| Related rules |
None |
| Fusion | Nter: <Unknown> Cter: None |
| Comments | Lysyl-tRNA synthesis is catalyzed by two unrelated families of tRNA synthetase; class-I or class-II. Usually only one or the other is present, however Methanosarcina seem to have both. Weird insertion in AQUAE. Unknown C-terminal domain in SYK2_MYCTU. |