HAMAP rule MF_00253
General rule information
[?]
| Accession | MF_00253 |
| Dates | 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 31) |
| Name | Gly_tRNA_synth |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P56206 (SYG_THET8); [ Recover all ] |
| Triggered by |
case c? <OC:Bacteria>
HAMAP; MF_00253_B (Get profile general information and statistics) end case
case c? <OC:Archaea>
HAMAP; MF_00253_A (Get profile general information and statistics) end case
|
Propagated annotation
[?]
Identifier, protein and gene names
[?]
| Identifier | SYG |
| Protein name | RecName: Full=Glycine--tRNA ligase; EC=6.1.1.14; AltName: Full=Glycyl-tRNA synthetase; Short=GlyRS; |
| case <OC:Bacteria> | |
| Gene name | Name=glyQS; |
| end case | |
| case <OC:Archaea> | |
| Gene name | Name=glyS; |
| end case | |
Comments
[?]
| FUNCTION | Catalyzes the attachment of glycine to tRNA(Gly). |
| CATALYTIC ACTIVITY | Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl- tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA- COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305, ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215; EC=6.1.1.14; |
| case <OC:Bacteria> | |
| SUBUNIT | Homodimer. |
| end case | |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the class-II aminoacyl-tRNA synthetase family. |
Keywords
[?]
Gene Ontology
[?]
| GO:0005524; Molecular function:ATP binding |
| GO:0004820; Molecular function:glycine-tRNA ligase activity |
| GO:0006426; Biological process:glycyl-tRNA aminoacylation |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
[?]
| Pfam | PF00587; tRNA-synt_2b; 1; |
| PRINTS | PR01043; TRNASYNTHGLY; 1; |
| NCBIfam | TIGR00389; GlyS_dimeric; 1; |
| PROSITE | PS50862; AA_TRNA_LIGASE_II; 1; |
Features
[?]
| From: SYG_THET8 (P56206) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| case <OC:Bacteria> | ||||||||||||
| BINDING | 221 | 223 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
R-N-E | ||||||||
| BINDING | 231 | 236 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[FILV]-R-x(3)-[FL] | ||||||||
| BINDING | 305 | 306 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
E-[CILV] | ||||||||
| BINDING | 364 | 367 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G-x(2)-R | ||||||||
| BINDING | 236 | 240 | /ligand="substrate" | [FL]-x-Q-x-E | ||||||||
| BINDING | 360 | 364 | /ligand="substrate" | E-x(3)-G | ||||||||
| BINDING | 99 | 99 | /ligand="substrate" | [KR] | ||||||||
| BINDING | 189 | 189 | /ligand="substrate" | E | ||||||||
| end case | ||||||||||||
| From: SYG_METJA (Q57681) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| case <OC:Archaea> | ||||||||||||
| BINDING | 196 | 198 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
R-N-E | ||||||||
| BINDING | 206 | 211 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[FILV]-R-x(3)-F | ||||||||
| BINDING | 328 | 329 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
E-[CILV] | ||||||||
| BINDING | 451 | 454 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G-x(2)-R | ||||||||
| BINDING | 211 | 215 | /ligand="substrate" | F-x(3)-E | ||||||||
| BINDING | 447 | 451 | /ligand="substrate" | E-x(3)-G | ||||||||
| BINDING | 98 | 98 | /ligand="substrate" | R | ||||||||
| BINDING | 164 | 164 | /ligand="substrate" | E | ||||||||
| end case | ||||||||||||
Additional information
[?]
| Size range | 565-583 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |