HAMAP rule MF_00278
General rule information
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| Accession | MF_00278 |
| Dates | 15-APR-2002 (Created)
28-JUN-2024 (Last updated, Version 50) |
| Name | HisH |
| Scope(s) |
Bacteria Archaea Plastid |
| Template(s) | P60595 (HIS5_ECOLI); Q9X0C8 (HIS5_THEMA); Q7SIC0 (HIS5_THET8); [ Recover all ] |
| Triggered by |
HAMAP; MF_00278 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | HIS5 |
| Protein name | RecName: Full=Imidazole glycerol phosphate synthase subunit HisH; EC=4.3.2.10; AltName: Full=IGP synthase glutaminase subunit; EC=3.5.1.2; AltName: Full=IGP synthase subunit HisH; AltName: Full=ImGP synthase subunit HisH; Short=IGPS subunit HisH; |
| Gene name | Name=hisH; |
Comments
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| FUNCTION | IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the synthesis of IGP and AICAR. The resulting ammonia molecule is channeled to the active site of HisF. |
| CATALYTIC ACTIVITY | Reaction=5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5- phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = 5- amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D- erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H(+) + L-glutamate; Xref=Rhea:RHEA:24793, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:58278, ChEBI:CHEBI:58359, ChEBI:CHEBI:58475, ChEBI:CHEBI:58525; EC=4.3.2.10; |
| CATALYTIC ACTIVITY | Reaction=H2O + L-glutamine = L-glutamate + NH4(+); Xref=Rhea:RHEA:15889, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:29985, ChEBI:CHEBI:58359; EC=3.5.1.2; |
| PATHWAY | Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9. |
| SUBUNIT | Heterodimer of HisH and HisF. |
| case <OG:Chloroplast> | |
| SUBCELLULAR LOCATION | Plastid, chloroplast. |
| else | |
| SUBCELLULAR LOCATION | Cytoplasm. |
| end case | |
Keywords
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| case not <OG:Chloroplast> | |
| Cytoplasm | |
| end case | |
| Amino-acid biosynthesis | |
| Histidine biosynthesis | |
| Hydrolase | |
| Glutamine amidotransferase | |
| Lyase | |
Gene Ontology
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| GO:0000107; Molecular function:imidazoleglycerol-phosphate synthase activity | |
| GO:0000105; Biological process:L-histidine biosynthetic process | |
| case <OG:Chloroplast> | |
| GO:0009507; Cellular component:chloroplast | |
| else; https://www.ebi.ac.uk/QuickGO/term/else | |
| GO:0005737; Cellular component:cytoplasm | |
| end case | |
Cross-references
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| Pfam | PF00117; GATase; 1; |
| PRINTS | PR00096; GATASE; 1; |
| NCBIfam | TIGR01855; IMP_synth_hisH; 1; |
| PROSITE | PS51273; GATASE_TYPE_1; 1; |
Features
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| From: HIS5_THEMA (Q9X0C8) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 84 | 84 | /note="Nucleophile" | C | ||||||||
| ACT_SITE | 178 | 178 | H | |||||||||
| ACT_SITE | 180 | 180 | E | |||||||||
Additional information
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| Size range | 186-242 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | AZOBR seems to lack an internal segment that contains the Cys active site; not shown in alignment |