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HAMAP rule MF_00278

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General rule information [?]

Accession MF_00278
Dates 15-APR-2002 (Created)
1-JUN-2023 (Last updated, Version 49)
Name HisH
Scope
Bacteria
Archaea
Plastid
Templates P60595 (HIS5_ECOLI); Q9X0C8 (HIS5_THEMA); Q7SIC0 (HIS5_THET8): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
HIS5
Protein name
RecName: Full=Imidazole glycerol phosphate synthase subunit HisH;
EC 4.3.2.10;
AltName: Full=IGP synthase glutaminase subunit;
EC 3.5.1.2;
AltName: Full=IGP synthase subunit HisH;
AltName: Full=ImGP synthase subunit HisH;
Short=IGPS subunit HisH;
Gene name
hisH

Comments [?]

Function IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the synthesis of IGP and AICAR. The resulting ammonia molecule is channeled to the active site of HisF.
Catalytic activity RHEA:24793: 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H(+) + L-glutamate
EC 4.3.2.10
RHEA:15889: H2O + L-glutamine = L-glutamate + NH4(+)
EC 3.5.1.2
Pathway Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.
Subunit Heterodimer of HisH and HisF.
case <OG:Chloroplast>
Subcellular location Plastid, chloroplast.
else
Subcellular location Cytoplasm.
end case

Keywords [?]

case not <OG:Chloroplast>
Cytoplasm
end case
Amino-acid biosynthesis
Histidine biosynthesis
Hydrolase
Glutamine amidotransferase
Lyase

Gene Ontology [?]

GO:0000107; Molecular function: imidazoleglycerol-phosphate synthase activity.
GO:0000105; Biological process: histidine biosynthetic process.
case <OG:Chloroplast>
GO:0009507; Cellular component: chloroplast.
else
GO:0005737; Cellular component: cytoplasm.
end case

Cross-references [?]

Pfam PF00117; GATase; 1;
PRINTS PR00096; GATASE; 1;
NCBIfam TIGR01855; IMP_synth_hisH; 1;
PROSITE PS51273; GATASE_TYPE_1; 1; trigger=PRU00605;

Features [?]

From: HIS5_THEMA (Q9X0C8)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     84     84       Nucleophile     C  
ACT_SITE     178     178             H  
ACT_SITE     180     180             E  

Additional information [?]

Size range 186-242 amino acids
Related rules None
Fusion None
Comments AZOBR seems to lack an internal segment that contains the Cys active site; not shown in alignment


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