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HAMAP rule MF_00278

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General rule information [?]

Accession MF_00278
Dates 15-APR-2002 (Created)
1-JUN-2023 (Last updated, Version 49)
Name HisH
Scope(s) Bacteria
Template(s) P60595 (HIS5_ECOLI); Q9X0C8 (HIS5_THEMA); Q7SIC0 (HIS5_THET8); [ Recover all ]
Triggered by HAMAP; MF_00278 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier HIS5
Protein name RecName: Full=Imidazole glycerol phosphate synthase subunit HisH;
AltName: Full=IGP synthase glutaminase subunit;
AltName: Full=IGP synthase subunit HisH;
AltName: Full=ImGP synthase subunit HisH;
                 Short=IGPS subunit HisH;
Gene name Name=hisH;

Comments [?]

FUNCTIONIGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the synthesis of IGP and AICAR. The resulting ammonia molecule is channeled to the active site of HisF.
CATALYTIC ACTIVITY Reaction=5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5- phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = 5- amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D- erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H(+) + L-glutamate; Xref=Rhea:RHEA:24793, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:58278, ChEBI:CHEBI:58359, ChEBI:CHEBI:58475, ChEBI:CHEBI:58525; EC=;
CATALYTIC ACTIVITY Reaction=H2O + L-glutamine = L-glutamate + NH4(+); Xref=Rhea:RHEA:15889, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938, ChEBI:CHEBI:29985, ChEBI:CHEBI:58359; EC=;
PATHWAYAmino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.
SUBUNITHeterodimer of HisH and HisF.
case <OG:Chloroplast>
SUBCELLULAR LOCATIONPlastid, chloroplast.
end case

Keywords [?]

Gene Ontology [?]

GO:0000107; Molecular function:imidazoleglycerol-phosphate synthase activity
GO:0000105; Biological process:histidine biosynthetic process
case <OG:Chloroplast>
GO:0009507; Cellular component:chloroplast
else; https://www.ebi.ac.uk/QuickGO/term/else
GO:0005737; Cellular component:cytoplasm
end case

Cross-references [?]

Pfam PF00117; GATase; 1;
NCBIfam TIGR01855; IMP_synth_hisH; 1;

Features [?]

From: HIS5_THEMA (Q9X0C8)
Key From To Description Tag Condition FTGroup
ACT_SITE 84 84 /note="Nucleophile" C
ACT_SITE 178 178 H
ACT_SITE 180 180 E

Additional information [?]

Size range 186-242 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments AZOBR seems to lack an internal segment that contains the Cys active site; not shown in alignment

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