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HAMAP rule MF_00281
General rule information
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| Accession | MF_00281 |
| Dates | 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 33) |
| Name | Phe_tRNA_synth_alpha1 |
| Scope(s) |
Bacteria |
| Template(s) | P08312 (SYFA_ECOLI); P27001 (SYFA_THETH); [ Recover all ] |
| Triggered by |
HAMAP; MF_00281 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | SYFA |
| Protein name | RecName: Full=Phenylalanine--tRNA ligase alpha subunit; EC=6.1.1.20; AltName: Full=Phenylalanyl-tRNA synthetase alpha subunit; Short=PheRS; |
| Gene name | Name=pheS; |
Comments
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| CATALYTIC ACTIVITY | Reaction=ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) + L-phenylalanyl-tRNA(Phe); Xref=Rhea:RHEA:19413, Rhea:RHEA-COMP:9668, Rhea:RHEA-COMP:9699, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:58095, ChEBI:CHEBI:78442, ChEBI:CHEBI:78531, ChEBI:CHEBI:456215; EC=6.1.1.20; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 2 magnesium ions per tetramer.; |
| SUBUNIT | Tetramer of two alpha and two beta subunits. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily. |
Keywords
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| Cytoplasm |
| Aminoacyl-tRNA synthetase |
| Protein biosynthesis |
| Ligase |
| ATP-binding |
| Nucleotide-binding |
| Magnesium |
| Metal-binding |
Gene Ontology
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| GO:0005524; Molecular function:ATP binding |
| GO:0000287; Molecular function:magnesium ion binding |
| GO:0004826; Molecular function:phenylalanine-tRNA ligase activity |
| GO:0006432; Biological process:phenylalanyl-tRNA aminoacylation |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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Features
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| From: SYFA_THETH (P27001) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 262 | 262 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_note="shared with beta subunit" |
E | ||||||||
Additional information
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| Size range | 325-373 amino acids |
| Related rules |
MF_00282 |
| Fusion | Nter: None Cter: None |
| Comments | BUCAI and BUCAP lack the conserved Glu that bind magnesium |