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Annotation rule MF_00281
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General rule information [?]

Accession MF_00281
Dates 1-JUN-2001 (Created)
18-NOV-2019 (Last updated, Version 29)
Name Phe_tRNA_synth_alpha1
Scope
Bacteria
Templates P08312 (SYFA_ECOLI); P27001 (SYFA_THETH): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
SYFA
Protein name
RecName: Full=Phenylalanine--tRNA ligase alpha subunit;
EC 6.1.1.20;
AltName: Full=Phenylalanyl-tRNA synthetase alpha subunit;
Short=PheRS;
Gene name
pheS

Comments [?]

Catalytic activity RHEA:19413: ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) + L-phenylalanyl-tRNA(Phe)
EC 6.1.1.20
Cofactor Mg(2+)
Note: Binds 2 magnesium ions per tetramer.
Subunit Tetramer of two alpha and two beta subunits.
Subcellular location Cytoplasm.
Similarity Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily.

Keywords [?]

Cytoplasm
Aminoacyl-tRNA synthetase
Protein biosynthesis
Ligase
ATP-binding
Nucleotide-binding
Magnesium
Metal-binding

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0004826; Molecular function: phenylalanine-tRNA ligase activity.
GO:0006432; Biological process: phenylalanyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF01409; tRNA-synt_2d; 1;
TIGRFAMs TIGR00468; PheS; 1;
PROSITE PS50862; AA_TRNA_LIGASE_II; 1;

Features [?]

From: SYFA_THETH (P27001)
Key     From     To       Description   Tag   Condition   FTGroup
METAL     262     262       Magnesium     E  

Additional information [?]

Size range 325-373 amino acids
Related rules MF_00282 (SYFA)
Fusion None
Comments BUCAI and BUCAP lack the conserved Glu that bind magnesium


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