HAMAP rule MF_00281

General rule information [?]

Accession	MF_00281
Dates	1-JUN-2001 (Created) 1-JUN-2023 (Last updated, Version 33)

Name	Phe_tRNA_synth_alpha1

Scope

Bacteria

Templates

P08312 (SYFA_ECOLI); P27001 (SYFA_THETH): [Recover all]

Triggered by

HAMAP; MF_00281 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

SYFA

Protein name

RecName:		Full=Phenylalanine--tRNA ligase alpha subunit;
		EC 6.1.1.20;
AltName:		Full=Phenylalanyl-tRNA synthetase alpha subunit;
		Short=PheRS;

Gene name

pheS

Comments [?]

Catalytic activity	RHEA:19413: ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) + L-phenylalanyl-tRNA(Phe) EC 6.1.1.20
Cofactor	Mg(2+) Note: Binds 2 magnesium ions per tetramer.
Subunit	Tetramer of two alpha and two beta subunits.
Subcellular location	Cytoplasm.
Similarity	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily.

Keywords [?]

Cytoplasm
Aminoacyl-tRNA synthetase
Protein biosynthesis
Ligase
ATP-binding
Nucleotide-binding
Magnesium
Metal-binding

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0004826; Molecular function: phenylalanine-tRNA ligase activity.
GO:0006432; Biological process: phenylalanyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam	PF01409; tRNA-synt_2d; 1;
NCBIfam	TIGR00468; PheS; 1;
PROSITE	PS50862; AA_TRNA_LIGASE_II; 1;

Features [?]

From: SYFA_THETH (P27001)

Key From To Description Tag Condition FTGroup

BINDING 262 262 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_note="shared with beta subunit E

Additional information [?]

Size range	325-373 amino acids
Related rules	MF_00282 (SYFA)
Fusion	None
Comments	BUCAI and BUCAP lack the conserved Glu that bind magnesium

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