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HAMAP rule MF_00281

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General rule information [?]

Accession MF_00281
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 33)
Name Phe_tRNA_synth_alpha1
Scope(s) Bacteria
Template(s) P08312 (SYFA_ECOLI); P27001 (SYFA_THETH); [ Recover all ]
Triggered by HAMAP; MF_00281 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier SYFA
Protein name RecName: Full=Phenylalanine--tRNA ligase alpha subunit;
                 EC=6.1.1.20;
AltName: Full=Phenylalanyl-tRNA synthetase alpha subunit;
                 Short=PheRS;
Gene name Name=pheS;

Comments [?]

CATALYTIC ACTIVITY Reaction=ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) + L-phenylalanyl-tRNA(Phe); Xref=Rhea:RHEA:19413, Rhea:RHEA-COMP:9668, Rhea:RHEA-COMP:9699, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:58095, ChEBI:CHEBI:78442, ChEBI:CHEBI:78531, ChEBI:CHEBI:456215; EC=6.1.1.20;
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 2 magnesium ions per tetramer.;
SUBUNITTetramer of two alpha and two beta subunits.
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYBelongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily.

Keywords [?]


Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
GO:0000287; Molecular function:magnesium ion binding
GO:0004826; Molecular function:phenylalanine-tRNA ligase activity
GO:0006432; Biological process:phenylalanyl-tRNA aminoacylation
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF01409; tRNA-synt_2d; 1;
NCBIfam TIGR00468; PheS; 1;
PROSITE PS50862; AA_TRNA_LIGASE_II; 1;

Features [?]

From: SYFA_THETH (P27001)
Key From To Description Tag Condition FTGroup
BINDING 262 262 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_note="shared with beta subunit"
E

Additional information [?]

Size range 325-373 amino acids
Related rules MF_00282
Fusion Nter: None Cter: None
Comments BUCAI and BUCAP lack the conserved Glu that bind magnesium



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