HAMAP rule MF_00295
General rule information
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Accession | MF_00295 |
Dates | 1-JUN-2001 (Created) 14-MAY-2022 (Last updated, Version 34) |
Name | MetA_acyltransf |
Scope | Bacteria |
Templates | Q72X44 (METAA_BACC1); P07623 (METAS_ECOLI); Q9WZY3 (METAA_THEMA); A0A1D3PCK2 (SAT_LACAI): [Recover all] |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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case <FTGroup:1> and ((<FTTag:P4> and <FTTag:P7>) or <FTTag:P5a>)
Identifier |
|
Protein name |
|
Gene name |
|
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10b> and not (<FTTag:P4> or <FTTag:P7>)
Identifier |
|
Protein name |
|
Gene name |
|
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10a> and not (<FTTag:P4> or <FTTag:P7>)
Identifier |
|
Protein name |
|
else
Identifier |
|
Protein name |
|
end case
Comments
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case <FTGroup:1> and ((<FTTag:P4> and <FTTag:P7>) or <FTTag:P5a>)
Function | Transfers a succinyl group from succinyl-CoA to L-homoserine, forming succinyl-L-homoserine. |
Catalytic activity | RHEA:22008: L-homoserine + succinyl-CoA = CoA + O-succinyl-L-homoserine
EC 2.3.1.46 |
Pathway | Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; O-succinyl-L-homoserine from L-homoserine: step 1/1. |
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10b> and not (<FTTag:P4> or <FTTag:P7>)
Function | Transfers an acetyl group from acetyl-CoA to L-homoserine, forming acetyl-L-homoserine. |
Catalytic activity | RHEA:13701: acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine
EC 2.3.1.31 |
Pathway | Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; O-acetyl-L-homoserine from L-homoserine: step 1/1. |
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10a> and not (<FTTag:P4> or <FTTag:P7>)
Function | Transfers an acetyl group from acetyl-CoA to L-serine, forming acetyl-L-serine. |
Catalytic activity | RHEA:24560: acetyl-CoA + L-serine = CoA + O-acetyl-L-serine
EC 2.3.1.30 |
Pathway | Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 1/2. |
end case
case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella>
Subunit | Homodimer. |
end case
Subcellular location | Cytoplasm. |
Similarity | Belongs to the MetA family. |
Keywords
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case <FTGroup:1> and ((<FTTag:P4> and <FTTag:P7>) or <FTTag:P5a>)
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10b> and not (<FTTag:P4> or <FTTag:P7>)
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10a> and not (<FTTag:P4> or <FTTag:P7>)
end case
Gene Ontology
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case <FTGroup:1> and ((<FTTag:P4> and <FTTag:P7>) or <FTTag:P5a>)
GO:0004414; Molecular function: homoserine O-acetyltransferase activity.
GO:0009086; Biological process: methionine biosynthetic process.
GO:0009086; Biological process: methionine biosynthetic process.
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10b> and not (<FTTag:P4> or <FTTag:P7>)
GO:0008899; Molecular function: homoserine O-succinyltransferase activity.
GO:0009086; Biological process: methionine biosynthetic process.
GO:0009086; Biological process: methionine biosynthetic process.
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10a> and not (<FTTag:P4> or <FTTag:P7>)
GO:0009001; Molecular function: serine O-acetyltransferase activity.
GO:0006535; Biological process: cysteine biosynthetic process from serine.
GO:0006535; Biological process: cysteine biosynthetic process from serine.
end case
GO:0005737; Cellular component: cytoplasm.
Cross-references
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Pfam | PF04204; HTS; 1; |
PIRSF | PIRSF000450; H_ser_succinyltr; 1; |
TIGRFAMs | TIGR01001; MetA; 1; |
Features
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From: METAA_BACC1 (Q72X44) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
ACT_SITE | 142 | 142 | Acyl-thioester intermediate | C | 1 | |||||||
ACT_SITE | 235 | 235 | Proton acceptor | H | 1 | |||||||
ACT_SITE | 237 | 237 | E | |||||||||
BINDING | 163 | 163 | /ligand="substrate | K | 1 | |||||||
BINDING (Optional) | 192 | 192 | /ligand="substrate | S | ||||||||
BINDING | 249 | 249 | /ligand="substrate | R | 1 | |||||||
SITE (Optional) | 109 | 109 | Important for acyl-CoA specificity | P4 | [NE] | |||||||
SITE (Optional) | 111 | 111 | Important for acyl-CoA specificity | P5a | [GA] | |||||||
SITE (Optional) | 111 | 111 | Important for acyl-CoA specificity | P5b | [ED] | |||||||
SITE (Optional) | 143 | 143 | Important for acyl-CoA specificity | P7 | L | |||||||
SITE (Optional) | 192 | 192 | Important for substrate specificity | P10a | A | |||||||
SITE (Optional) | 192 | 192 | Important for substrate specificity | P10b | S |
Additional information
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Size range | 250-385 amino acids |
Related rules | None |
Fusion | None |
Comments | Classification as HST, HAT or SAT was done according to Bastard et al. (2017) (PubMed:28581482). |