HAMAP rule MF_00295
General rule information
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| Accession | MF_00295 |
| Dates | 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 35) |
| Name | MetA_acyltransf |
| Scope(s) |
Bacteria |
| Template(s) | Q72X44 (METAA_BACC1); P07623 (METAS_ECOLI); Q9WZY3 (METAA_THEMA); A0A1D3PCK2 (SAT_LACAI); [ Recover all ] |
| Triggered by |
HAMAP; MF_00295 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| case <FTGroup:1> and ((<FTTag:P4> and <FTTag:P7>) or <FTTag:P5a>) | |
| Identifier | METAS |
| Protein name | RecName: Full=Homoserine O-succinyltransferase; Short=HST; EC=2.3.1.46; AltName: Full=Homoserine transsuccinylase; Short=HTS; |
| Gene name | Name=metAS; |
| else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10b> and not (<FTTag:P4> or <FTTag:P7>) | |
| Identifier | METAA |
| Protein name | RecName: Full=Homoserine O-acetyltransferase; Short=HAT; EC=2.3.1.31; AltName: Full=Homoserine transacetylase; Short=HTA; |
| Gene name | Name=metAA; |
| else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10a> and not (<FTTag:P4> or <FTTag:P7>) | |
| Identifier | SAT |
| Protein name | RecName: Full=Serine O-acetyltransferase; Short=SAT; EC=2.3.1.30; |
| else | |
| Identifier | META |
| Protein name | RecName: Full=Probable acyltransferase; EC=2.3.1.-; |
| end case | |
Comments
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| case <FTGroup:1> and ((<FTTag:P4> and <FTTag:P7>) or <FTTag:P5a>) | |
| FUNCTION | Transfers a succinyl group from succinyl-CoA to L-homoserine, forming succinyl-L-homoserine. |
| CATALYTIC ACTIVITY | Reaction=L-homoserine + succinyl-CoA = CoA + O-succinyl-L-homoserine; Xref=Rhea:RHEA:22008, ChEBI:CHEBI:57287, ChEBI:CHEBI:57292, ChEBI:CHEBI:57476, ChEBI:CHEBI:57661; EC=2.3.1.46; |
| PATHWAY | Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; O-succinyl-L-homoserine from L-homoserine: step 1/1. |
| else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10b> and not (<FTTag:P4> or <FTTag:P7>) | |
| FUNCTION | Transfers an acetyl group from acetyl-CoA to L-homoserine, forming acetyl-L-homoserine. |
| CATALYTIC ACTIVITY | Reaction=acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine; Xref=Rhea:RHEA:13701, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288, ChEBI:CHEBI:57476, ChEBI:CHEBI:57716; EC=2.3.1.31; |
| PATHWAY | Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; O-acetyl-L-homoserine from L-homoserine: step 1/1. |
| else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10a> and not (<FTTag:P4> or <FTTag:P7>) | |
| FUNCTION | Transfers an acetyl group from acetyl-CoA to L-serine, forming acetyl-L-serine. |
| CATALYTIC ACTIVITY | Reaction=acetyl-CoA + L-serine = CoA + O-acetyl-L-serine; Xref=Rhea:RHEA:24560, ChEBI:CHEBI:33384, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288, ChEBI:CHEBI:58340; EC=2.3.1.30; |
| PATHWAY | Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 1/2. |
| end case | |
| case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella> | |
| SUBUNIT | Homodimer. |
| end case | |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the MetA family. |
Keywords
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| Cytoplasm | |
| Amino-acid biosynthesis | |
| case <FTGroup:1> and ((<FTTag:P4> and <FTTag:P7>) or <FTTag:P5a>) | |
| Methionine biosynthesis | |
| else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10b> and not (<FTTag:P4> or <FTTag:P7>) | |
| Methionine biosynthesis | |
| else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10a> and not (<FTTag:P4> or <FTTag:P7>) | |
| Cysteine biosynthesis | |
| end case | |
| Transferase | |
| Acyltransferase | |
Gene Ontology
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| case <FTGroup:1> and ((<FTTag:P4> and <FTTag:P7>) or <FTTag:P5a>) | |
| GO:0004414; Molecular function:homoserine O-acetyltransferase activity | |
| GO:0009086; Biological process:methionine biosynthetic process | |
| else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10b> and not (<FTTag:P4> or <FTTag:P7>) | |
| GO:0008899; Molecular function:homoserine O-succinyltransferase activity | |
| GO:0009086; Biological process:methionine biosynthetic process | |
| else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10a> and not (<FTTag:P4> or <FTTag:P7>) | |
| GO:0009001; Molecular function:serine O-acetyltransferase activity | |
| GO:0006535; Biological process:cysteine biosynthetic process from serine | |
| end case | |
| GO:0005737; Cellular component:cytoplasm | |
Cross-references
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| Pfam | PF04204; HTS; 1; |
| PIRSF | PIRSF000450; H_ser_succinyltr; 1; |
| NCBIfam | TIGR01001; MetA; 1; |
Features
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| From: METAA_BACC1 (Q72X44) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 142 | 142 | /note="Acyl-thioester intermediate" | C | 1 | |||||||
| ACT_SITE | 235 | 235 | /note="Proton acceptor" | H | 1 | |||||||
| ACT_SITE | 237 | 237 | E | |||||||||
| BINDING | 163 | 163 | /ligand="substrate" | K | 1 | |||||||
| BINDING | 192 | 192 | /ligand="substrate" | S | ||||||||
| BINDING | 249 | 249 | /ligand="substrate" | R | 1 | |||||||
| SITE | 109 | 109 | /note="Important for acyl-CoA specificity" | P4 | [NE] | |||||||
| SITE | 111 | 111 | /note="Important for acyl-CoA specificity" | P5a | [GA] | |||||||
| SITE | 111 | 111 | /note="Important for acyl-CoA specificity" | P5b | [ED] | |||||||
| SITE | 143 | 143 | /note="Important for acyl-CoA specificity" | P7 | L | |||||||
| SITE | 192 | 192 | /note="Important for substrate specificity" | P10a | A | |||||||
| SITE | 192 | 192 | /note="Important for substrate specificity" | P10b | S | |||||||
Additional information
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| Size range | 250-385 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | Classification as HST, HAT or SAT was done according to Bastard et al. (2017) (PubMed:28581482). |