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Annotation rule MF_00295
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General rule information [?]

Accession MF_00295
Dates 1-JUN-2001 (Created)
18-NOV-2019 (Last updated, Version 33)
Name MetA_acyltransf
Scope
Bacteria
Templates Q72X44 (METAA_BACC1); P07623 (METAS_ECOLI); Q9WZY3 (METAA_THEMA); A0A1D3PCK2 (SAT_LACAI): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

case <FTGroup:1> and ((<FTTag:P4> and <FTTag:P7>) or <FTTag:P5a>)
Identifier
METAS
Protein name
RecName: Full=Homoserine O-succinyltransferase;
Short=HST;
EC 2.3.1.46;
AltName: Full=Homoserine transsuccinylase;
Short=HTS;
Gene name
metAS
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10b> and not (<FTTag:P4> or <FTTag:P7>)
Identifier
METAA
Protein name
RecName: Full=Homoserine O-acetyltransferase;
Short=HAT;
EC 2.3.1.31;
AltName: Full=Homoserine transacetylase;
Short=HTA;
Gene name
metAA
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10a> and not (<FTTag:P4> or <FTTag:P7>)
Identifier
SAT
Protein name
RecName: Full=Serine O-acetyltransferase;
Short=SAT;
EC 2.3.1.30;
else
Identifier
META
Protein name
RecName: Full=Probable acyltransferase;
EC 2.3.1.-;
end case

Comments [?]

case <FTGroup:1> and ((<FTTag:P4> and <FTTag:P7>) or <FTTag:P5a>)
Function Transfers a succinyl group from succinyl-CoA to L-homoserine, forming succinyl-L-homoserine.
Catalytic activity RHEA:22008: L-homoserine + succinyl-CoA = CoA + O-succinyl-L-homoserine
EC 2.3.1.46
Pathway Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; O-succinyl-L-homoserine from L-homoserine: step 1/1.
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10b> and not (<FTTag:P4> or <FTTag:P7>)
Function Transfers an acetyl group from acetyl-CoA to L-homoserine, forming acetyl-L-homoserine.
Catalytic activity RHEA:13701: acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine
EC 2.3.1.31
Pathway Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; O-acetyl-L-homoserine from L-homoserine: step 1/1.
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10a> and not (<FTTag:P4> or <FTTag:P7>)
Function Transfers an acetyl group from acetyl-CoA to L-serine, forming acetyl-L-serine.
Catalytic activity RHEA:24560: acetyl-CoA + L-serine = CoA + O-acetyl-L-serine
EC 2.3.1.30
Pathway Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 1/2.
end case
case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella>
Subunit Homodimer.
end case
Subcellular location Cytoplasm.
Similarity Belongs to the MetA family.

Keywords [?]

Cytoplasm
Amino-acid biosynthesis
case <FTGroup:1> and ((<FTTag:P4> and <FTTag:P7>) or <FTTag:P5a>)
Methionine biosynthesis
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10b> and not (<FTTag:P4> or <FTTag:P7>)
Methionine biosynthesis
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10a> and not (<FTTag:P4> or <FTTag:P7>)
Cysteine biosynthesis
end case
Transferase
Acyltransferase

Gene Ontology [?]

case <FTGroup:1> and ((<FTTag:P4> and <FTTag:P7>) or <FTTag:P5a>)
GO:0004414; Molecular function: homoserine O-acetyltransferase activity.
GO:0009086; Biological process: methionine biosynthetic process.
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10b> and not (<FTTag:P4> or <FTTag:P7>)
GO:0008899; Molecular function: homoserine O-succinyltransferase activity.
GO:0009086; Biological process: methionine biosynthetic process.
else case <FTGroup:1> and <FTTag:P5b> and <FTTag:P10a> and not (<FTTag:P4> or <FTTag:P7>)
GO:0009001; Molecular function: serine O-acetyltransferase activity.
GO:0006535; Biological process: cysteine biosynthetic process from serine.
end case
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF04204; HTS; 1;
PIRSF PIRSF000450; H_ser_succinyltr; 1;
TIGRFAMs TIGR01001; MetA; 1;

Features [?]

From: METAA_BACC1 (Q72X44)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     142     142       Acyl-thioester intermediate     C   1
ACT_SITE     235     235       Proton acceptor     H   1
ACT_SITE     237     237             E  
BINDING     163     163       Substrate     K   1
BINDING (Optional)     192     192       Substrate     S  
BINDING     249     249       Substrate     R   1
SITE (Optional)     109     109       Important for acyl-CoA specificity   P4   [NE]  
SITE (Optional)     111     111       Important for acyl-CoA specificity   P5a   [GA]  
SITE (Optional)     111     111       Important for acyl-CoA specificity   P5b   [ED]  
SITE (Optional)     143     143       Important for acyl-CoA specificity   P7   L  
SITE (Optional)     192     192       Important for substrate specificity   P10a   A  
SITE (Optional)     192     192       Important for substrate specificity   P10b   S  

Additional information [?]

Size range 250-385 amino acids
Related rules None
Fusion None
Comments Classification as HST, HAT or SAT was done according to Bastard et al. (2017) (PubMed:28581482).


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