HAMAP rule MF_00296
General rule information
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| Accession | MF_00296 |
| Dates | 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 42) |
| Name | MetX_acyltransf |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P45131 (METXA_HAEIN); Q8F4I0 (METXA_LEPIN); O68640 (METXA_CORGL); Q9RA51 (METXA_THET2); Q6FEQ3 (METXS_ACIAD); P57714 (METXS_PSEAE); Q8P8L2 (SST_XANCP); Q8TME4 (METXA_METAC); [ Recover all ] |
| Triggered by |
HAMAP; MF_00296 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| case <FTGroup:1> and not (<FTTag:P11> or <FTTag:P17>) | |
| Identifier | METXA |
| Protein name | RecName: Full=Homoserine O-acetyltransferase; Short=HAT; EC=2.3.1.31; AltName: Full=Homoserine transacetylase; Short=HTA; |
| Gene name | Name=metXA; |
| else case <FTGroup:1> and <FTTag:P17> | |
| Identifier | METXS |
| Protein name | RecName: Full=Homoserine O-succinyltransferase; Short=HST; EC=2.3.1.46; AltName: Full=Homoserine transsuccinylase; Short=HTS; |
| Gene name | Name=metXS; |
| else case <FTGroup:1> and <FTTag:P11> and <FTTag:P1P4> and <FTTag:P9> | |
| Identifier | METXS |
| Protein name | RecName: Full=Homoserine O-succinyltransferase; Short=HST; EC=2.3.1.46; AltName: Full=Homoserine transsuccinylase; Short=HTS; |
| Gene name | Name=metXS; |
| else case <FTGroup:1> and <FTTag:P11> and <FTTag:P1P4> and not <FTTag:P9> | |
| Identifier | SST |
| Protein name | RecName: Full=Serine O-succinyltransferase; Short=SST; EC=2.3.1.-; |
| else | |
| Identifier | METX |
| Protein name | RecName: Full=Probable acyltransferase; EC=2.3.1.-; |
| end case | |
Comments
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| case <FTGroup:1> and not (<FTTag:P11> or <FTTag:P17>) | |
| FUNCTION | Transfers an acetyl group from acetyl-CoA to L-homoserine, forming acetyl-L-homoserine. |
| CATALYTIC ACTIVITY | Reaction=acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine; Xref=Rhea:RHEA:13701, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288, ChEBI:CHEBI:57476, ChEBI:CHEBI:57716; EC=2.3.1.31; |
| PATHWAY | Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; O-acetyl-L-homoserine from L-homoserine: step 1/1. |
| else case (<FTGroup:1> and <FTTag:P17>) or (<FTGroup:1> and <FTTag:P11> and <FTTag:P1P4> and <FTTag:P9>) | |
| FUNCTION | Transfers a succinyl group from succinyl-CoA to L-homoserine, forming succinyl-L-homoserine. |
| CATALYTIC ACTIVITY | Reaction=L-homoserine + succinyl-CoA = CoA + O-succinyl-L-homoserine; Xref=Rhea:RHEA:22008, ChEBI:CHEBI:57287, ChEBI:CHEBI:57292, ChEBI:CHEBI:57476, ChEBI:CHEBI:57661; EC=2.3.1.46; |
| PATHWAY | Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; O-succinyl-L-homoserine from L-homoserine: step 1/1. |
| else case <FTGroup:1> and <FTTag:P11> and <FTTag:P1P4> and not <FTTag:P9> | |
| FUNCTION | Transfers a succinyl group from succinyl-CoA to L-serine, forming succinyl-L-serine. |
| CATALYTIC ACTIVITY | Reaction=L-serine + succinyl-CoA = CoA + O-succinyl-L-serine; Xref=Rhea:RHEA:52820, ChEBI:CHEBI:33384, ChEBI:CHEBI:57287, ChEBI:CHEBI:57292, ChEBI:CHEBI:136856; |
| PATHWAY | Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 1/2. |
| end case | |
| SUBUNIT | Homodimer. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the AB hydrolase superfamily. MetX family. |
Keywords
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| Cytoplasm | |
| Amino-acid biosynthesis | |
| case <FTGroup:1> and not (<FTTag:P11> or <FTTag:P17>) | |
| Methionine biosynthesis | |
| else case (<FTGroup:1> and <FTTag:P17>) or (<FTGroup:1> and <FTTag:P11> and <FTTag:P1P4> and <FTTag:P9>) | |
| Methionine biosynthesis | |
| else case <FTGroup:1> and <FTTag:P11> and <FTTag:P1P4> and not <FTTag:P9> | |
| Cysteine biosynthesis | |
| end case | |
| Transferase | |
| Acyltransferase | |
Gene Ontology
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| case <FTGroup:1> and not (<FTTag:P11> or <FTTag:P17>) | |
| GO:0004414; Molecular function:homoserine O-acetyltransferase activity | |
| GO:0009086; Biological process:methionine biosynthetic process | |
| else case (<FTGroup:1> and <FTTag:P17>) or (<FTGroup:1> and <FTTag:P11> and <FTTag:P1P4> and <FTTag:P9>) | |
| GO:0008899; Molecular function:homoserine O-succinyltransferase activity | |
| GO:0009086; Biological process:methionine biosynthetic process | |
| else case <FTGroup:1> and <FTTag:P11> and <FTTag:P1P4> and not <FTTag:P9> | |
| GO:0016750; Molecular function:O-succinyltransferase activity | |
| GO:0006535; Biological process:cysteine biosynthetic process from serine | |
| end case | |
| GO:0005737; Cellular component:cytoplasm | |
Cross-references
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| Pfam | PF00561; Abhydrolase_1; 1; |
| PIRSF | PIRSF000443; Homoser_Ac_trans; 1; |
| NCBIfam | TIGR01392; homoserO_Ac_trn; 1; |
| PROSITE | PS51371; CBS; 0-2; |
Features
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| From: METXA_HAEIN (P45131) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| REGION | 48 | 51 | /note="Important for substrate specificity" | P1P4 | G-[LI]-S-[PA] | |||||||
| ACT_SITE | 143 | 143 | /note="Nucleophile" | S | 1 | |||||||
| ACT_SITE | 304 | 304 | D | |||||||||
| ACT_SITE | 337 | 337 | H | 1 | ||||||||
| BINDING | 212 | 212 | /ligand="substrate" | R | 1 | |||||||
| BINDING | 338 | 338 | /ligand="substrate" | D | 1 | |||||||
| SITE | 144 | 144 | /note="Important for substrate specificity" | P9 | Y | |||||||
| SITE | 180 | 180 | /note="Important for acyl-CoA specificity" | P11 | R | |||||||
| SITE | 306 | 306 | /note="Important for acyl-CoA specificity" | P17 | R | |||||||
Additional information
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| Size range | 315-545 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | Classification as HST, HAT or SST was done according to Bastard et al. (2017) (PubMed:28581482). |