HAMAP rule MF_00306

Accession	MF_00306
Dates	1-JUN-2001 (Created) 1-JUN-2023 (Last updated, Version 35)

Name

SRP54

Scope

Bacteria Archaea

Templates

P0AGD7 (SRP54_ECOLI); O29633 (SRP54_ARCFU); P37105 (SRP54_BACSU); O07347 (SRP54_THEAQ); Q977V2 (SRP54_HALVD): [Recover all]

Triggered by

HAMAP; MF_00306 (Get profile general information and statistics)

Identifier

SRP54

Protein name

RecName: Full=Signal recognition particle 54 kDa protein; Short=SRP54; EC 3.6.5.4;

Gene name

srp54

Protein name

RecName: Full=Signal recognition particle protein; EC 3.6.5.4; AltName: Full=Fifty-four homolog;

Gene name

ffh

Function	Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY.
Catalytic activity	RHEA:19669: GTP + H2O = GDP + H(+) + phosphate EC 3.6.5.4
Subunit	Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. Archaeal SRP consists of a 7S RNA molecule of 300 nucleotides and two protein subunits: SRP54 and SRP19.

Function	Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. Interaction with FtsY leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components.
Catalytic activity	RHEA:19669: GTP + H2O = GDP + H(+) + phosphate EC 3.6.5.4
Subunit	Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. SRP is a ribonucleoprotein composed of Ffh and a 4.5S RNA molecule.

Function	Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY.
Catalytic activity	RHEA:19669: GTP + H2O = GDP + H(+) + phosphate EC 3.6.5.4
Subunit	Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY.

Subcellular location	Cytoplasm. Note=The SRP-RNC complex is targeted to the cytoplasmic membrane.
Domain	Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.
Similarity	Belongs to the GTP-binding SRP family. SRP54 subfamily.

Cytoplasm
Signal recognition particle
GTP-binding
Hydrolase
Nucleotide-binding
Ribonucleoprotein
RNA-binding

GO:0005525; Molecular function: GTP binding.
GO:0003924; Molecular function: GTPase activity.

GO:0008312; Molecular function: 7S RNA binding.

GO:0003723; Molecular function: RNA binding.

GO:0006612; Biological process: protein targeting to membrane.
GO:0048500; Cellular component: signal recognition particle.
GO:1990904; Cellular component: ribonucleoprotein complex.

Pfam	PF00448; SRP54; 1;
	PF02881; SRP54_N; 1;
	PF02978; SRP_SPB; 1;
PROSITE	PS00300; SRP54; 1;
NCBIfam	TIGR00959; Ffh; 1;

From: SRP54_METJA (Q57565)

Key

From

To

Description

Tag

Condition

FTGroup

BINDING

107

114

/ligand="GTP" /ligand_id="ChEBI:CHEBI:37565

G-x-x-G-x(1,3)-G-K-T

BINDING

188

192

/ligand="GTP" /ligand_id="ChEBI:CHEBI:37565

D-[ST]-[AS]-G-R

BINDING

247

250

/ligand="GTP" /ligand_id="ChEBI:CHEBI:37565

x-[KR]-x-D

Size range	430-525 amino acids
Related rules	None
Fusion	None

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