HAMAP rule MF_00316
General rule information
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| Accession | MF_00316 |
| Dates | 1-JUN-2001 (Created)
2-SEP-2024 (Last updated, Version 36) |
| Name | MobA |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P32173 (MOBA_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_00316 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | MOBA |
| case <OC:Pseudomonadota> | |
| Protein name | RecName: Full=Molybdenum cofactor guanylyltransferase; Short=MoCo guanylyltransferase; EC=2.7.7.77; AltName: Full=GTP:molybdopterin guanylyltransferase; AltName: Full=Molybdopterin-guanine dinucleotide synthase; Short=MGD synthase; AltName: Full=Molybdopterin guanylyltransferase; AltName: Full=Mo-MPT guanylyltransferase; |
| else | |
| Protein name | RecName: Full=Probable molybdenum cofactor guanylyltransferase; Short=MoCo guanylyltransferase; EC=2.7.7.77; AltName: Full=GTP:molybdopterin guanylyltransferase; AltName: Full=Molybdopterin-guanine dinucleotide synthase; Short=MGD synthase; AltName: Full=Molybdopterin guanylyltransferase; AltName: Full=Mo-MPT guanylyltransferase; |
| end case | |
| Gene name | Name=mobA; |
Comments
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| FUNCTION | Transfers a GMP moiety from GTP to Mo-molybdopterin (Mo-MPT) cofactor (Moco or molybdenum cofactor) to form Mo-molybdopterin guanine dinucleotide (Mo-MGD) cofactor. |
| CATALYTIC ACTIVITY | Reaction=Mo-molybdopterin + GTP + H(+) = Mo-molybdopterin guanine dinucleotide + diphosphate; Xref=Rhea:RHEA:34243, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:37565, ChEBI:CHEBI:71302, ChEBI:CHEBI:71310; EC=2.7.7.77; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| case <OC:Pseudomonadota> | |
| SUBUNIT | Monomer. |
| end case | |
| DOMAIN | The N-terminal domain determines nucleotide recognition and specific binding, while the C-terminal domain determines the specific binding to the target protein. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the MobA family. |
Keywords
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| Cytoplasm |
| GTP-binding |
| Magnesium |
| Metal-binding |
| Molybdenum cofactor biosynthesis |
| Nucleotide-binding |
| Transferase |
Gene Ontology
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| GO:0005525; Molecular function:GTP binding |
| GO:0070568; Molecular function:guanylyltransferase activity |
| GO:0019720; Biological process:Mo-molybdopterin cofactor metabolic process |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| NCBIfam | TIGR02665; Molyb_mobA; 1; |
Features
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| From: MOBA_ECOLI (P32173) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 12 | 14 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
[LFIVT]-x-G | ||||||||
| BINDING | 101 | 101 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | ||||||||
| BINDING | 25 | 25 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
[KR] | ||||||||
| BINDING | 53 | 53 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
[NRHK] | ||||||||
| BINDING | 71 | 71 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
D | ||||||||
| BINDING | 101 | 101 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
D | ||||||||
Additional information
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| Size range | 189-225 amino acids |
| Related rules |
None |
| Fusion | Nter: MF_01224 (moaC) Cter: None |